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Literature summary extracted from

  • Bertsova, Y.V.; Fadeeva, M.S.; Kostyrko, V.A.; Serebryakova, M.V.; Baykov, A.A.; Bogachev, A.V.
    Alternative pyrimidine biosynthesis protein ApbE is a flavin transferase catalyzing covalent attachment of FMN to a threonine residue in bacterial flavoproteins (2013), J. Biol. Chem., 288, 14276-14286 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.1.1 nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity Vibrio harveyi
7.2.1.1 nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.1.1 membrane
-
 Vibrio harveyi 16020
-
7.2.1.1 membrane
-
 Klebsiella pneumoniae 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio harveyi
-
 NAD+ + ubiquinol + n Na+/out
-
 r
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Klebsiella pneumoniae
-
 NAD+ + ubiquinol + n Na+/out
-
 r
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Vibrio harveyi R3
-
 NAD+ + ubiquinol + n Na+/out
-
 r
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in Klebsiella pneumoniae 204
-
 NAD+ + ubiquinol + n Na+/out
-
 r

Organism

EC Number Organism UniProt Comment Textmining
7.2.1.1 Klebsiella pneumoniae
-
-
-
7.2.1.1 Klebsiella pneumoniae 204
-
-
-
7.2.1.1 Vibrio harveyi
-
-
-
7.2.1.1 Vibrio harveyi R3
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio harveyi NAD+ + ubiquinol + n Na+/out
-
 r
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Klebsiella pneumoniae NAD+ + ubiquinol + n Na+/out
-
 r
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Vibrio harveyi R3 NAD+ + ubiquinol + n Na+/out
-
 r
7.2.1.1 NADH + H+ + ubiquinone + n Na+/in
-
 Klebsiella pneumoniae 204 NAD+ + ubiquinol + n Na+/out
-
 r

Subunits

EC Number Subunits Comment Organism
7.2.1.1 hexamer Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon Vibrio harveyi
7.2.1.1 hexamer Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
7.2.1.1 Na+-NQR
-
 Vibrio harveyi
7.2.1.1 Na+-NQR
-
 Klebsiella pneumoniae
7.2.1.1 Na+-translocating NADH:quinone oxidoreductase
-
 Vibrio harveyi
7.2.1.1 Na+-translocating NADH:quinone oxidoreductase
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
7.2.1.1 FAD Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o f NqrC is Mg2+-dependent and proceeds with FAD but not FMN Klebsiella pneumoniae
7.2.1.1 FAD Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, it catalyzes the flavinylation of truncated Vibrio harveyi NqrCsubunit of enzyme Na+-NQR at Thr229, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o fNqrC isMg2-dependent and proceeds with FAD but not FMN Vibrio harveyi
7.2.1.1 FMN Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin Vibrio harveyi
7.2.1.1 FMN Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin Klebsiella pneumoniae
7.2.1.1 NAD+
-
 Vibrio harveyi
7.2.1.1 NAD+
-
 Klebsiella pneumoniae
7.2.1.1 NADH
-
 Vibrio harveyi
7.2.1.1 NADH
-
 Klebsiella pneumoniae
7.2.1.1 riboflavin the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB Vibrio harveyi
7.2.1.1 riboflavin the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB Klebsiella pneumoniae
7.2.1.1 [2Fe-2S]-center the enzyme contains one [2Fe-2S] cluster in subunit NqrF Vibrio harveyi
7.2.1.1 [2Fe-2S]-center the enzyme contains one [2Fe-2S] cluster in subunit NqrF Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
7.2.1.1 malfunction inactivation of the apbE gene in Klebsiella pneumoniae, wherein the nqr operon and apbE are well separated in the chromosome, results in a complete loss of the quinone reductase activity of Na+-NQR Klebsiella pneumoniae
7.2.1.1 additional information the enzyme shows dependence on covalently bound flavin Vibrio harveyi
7.2.1.1 additional information the enzyme shows dependence on covalently bound flavin Klebsiella pneumoniae