BRENDA - Enzyme Database

Alternative pyrimidine biosynthesis protein ApbE is a flavin transferase catalyzing covalent attachment of FMN to a threonine residue in bacterial flavoproteins

Bertsova, Y.V.; Fadeeva, M.S.; Kostyrko, V.A.; Serebryakova, M.V.; Baykov, A.A.; Bogachev, A.V.; J. Biol. Chem. 288, 14276-14286 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.2.1.1
nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity
Klebsiella pneumoniae
7.2.1.1
nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity
Vibrio harveyi
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Klebsiella pneumoniae
16020
-
7.2.1.1
membrane
-
Vibrio harveyi
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio harveyi
-
NAD+ + ubiquinol + n Na+/out
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Klebsiella pneumoniae
-
NAD+ + ubiquinol + n Na+/out
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio harveyi R3
-
NAD+ + ubiquinol + n Na+/out
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Klebsiella pneumoniae 204
-
NAD+ + ubiquinol + n Na+/out
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.2.1.1
Klebsiella pneumoniae
-
-
-
7.2.1.1
Klebsiella pneumoniae 204
-
-
-
7.2.1.1
Vibrio harveyi
-
-
-
7.2.1.1
Vibrio harveyi R3
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Vibrio harveyi
NAD+ + ubiquinol + n Na+/out
-
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Klebsiella pneumoniae
NAD+ + ubiquinol + n Na+/out
-
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Vibrio harveyi R3
NAD+ + ubiquinol + n Na+/out
-
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Klebsiella pneumoniae 204
NAD+ + ubiquinol + n Na+/out
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
7.2.1.1
hexamer
Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon
Klebsiella pneumoniae
7.2.1.1
hexamer
Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon
Vibrio harveyi
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o f NqrC is Mg2+-dependent and proceeds with FAD but not FMN
Klebsiella pneumoniae
7.2.1.1
FAD
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, it catalyzes the flavinylation of truncated Vibrio harveyi NqrCsubunit of enzyme Na+-NQR at Thr229, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o fNqrC isMg2-dependent and proceeds with FAD but not FMN
Vibrio harveyi
7.2.1.1
FMN
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin
Klebsiella pneumoniae
7.2.1.1
FMN
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin
Vibrio harveyi
7.2.1.1
NAD+
-
Klebsiella pneumoniae
7.2.1.1
NAD+
-
Vibrio harveyi
7.2.1.1
NADH
-
Klebsiella pneumoniae
7.2.1.1
NADH
-
Vibrio harveyi
7.2.1.1
riboflavin
the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB
Klebsiella pneumoniae
7.2.1.1
riboflavin
the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB
Vibrio harveyi
7.2.1.1
[2Fe-2S]-center
the enzyme contains one [2Fe-2S] cluster in subunit NqrF
Klebsiella pneumoniae
7.2.1.1
[2Fe-2S]-center
the enzyme contains one [2Fe-2S] cluster in subunit NqrF
Vibrio harveyi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.2.1.1
nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity
Klebsiella pneumoniae
7.2.1.1
nqr operon encoding all subunits A-F, genetic structure, recombinant expression of His6-tagged subunit NqrC in Escherichia coli, coexpression of flavin transferase ApbE is required for Na+-NQR enzyme activity
Vibrio harveyi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.2.1.1
FAD
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o f NqrC is Mg2+-dependent and proceeds with FAD but not FMN
Klebsiella pneumoniae
7.2.1.1
FAD
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains one noncovalently bound FAD molecule in subunit NqrF. The enzyme shows dependence on covalently bound flavin. ApbE is a modifying enzyme involved in the maturation of flavoproteins, which catalyzes Mg2+-dependent FMN transfer from FAD to Thr residues of flavoproteins in vitro and in vivo, it catalyzes the flavinylation of truncated Vibrio harveyi NqrCsubunit of enzyme Na+-NQR at Thr229, ApbE is the only protein factor required for NqrC flavinylation. Flavinylation o fNqrC isMg2-dependent and proceeds with FAD but not FMN
Vibrio harveyi
7.2.1.1
FMN
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin
Klebsiella pneumoniae
7.2.1.1
FMN
Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) contains two FMN residues as redox-active prosthetic groups attached by a phosphoester bond to threonine residues in subunits NqrB and NqrC. The enzyme shows dependence on covalently bound flavin
Vibrio harveyi
7.2.1.1
NAD+
-
Klebsiella pneumoniae
7.2.1.1
NAD+
-
Vibrio harveyi
7.2.1.1
NADH
-
Klebsiella pneumoniae
7.2.1.1
NADH
-
Vibrio harveyi
7.2.1.1
riboflavin
the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB
Klebsiella pneumoniae
7.2.1.1
riboflavin
the enzyme contains one noncovalently bound riboflavin molecule in subunit NqrB
Vibrio harveyi
7.2.1.1
[2Fe-2S]-center
the enzyme contains one [2Fe-2S] cluster in subunit NqrF
Klebsiella pneumoniae
7.2.1.1
[2Fe-2S]-center
the enzyme contains one [2Fe-2S] cluster in subunit NqrF
Vibrio harveyi
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.2.1.1
membrane
-
Klebsiella pneumoniae
16020
-
7.2.1.1
membrane
-
Vibrio harveyi
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio harveyi
-
NAD+ + ubiquinol + n Na+/out
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Klebsiella pneumoniae
-
NAD+ + ubiquinol + n Na+/out
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Vibrio harveyi R3
-
NAD+ + ubiquinol + n Na+/out
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
Klebsiella pneumoniae 204
-
NAD+ + ubiquinol + n Na+/out
-
-
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Vibrio harveyi
NAD+ + ubiquinol + n Na+/out
-
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Klebsiella pneumoniae
NAD+ + ubiquinol + n Na+/out
-
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Vibrio harveyi R3
NAD+ + ubiquinol + n Na+/out
-
-
-
r
7.2.1.1
NADH + H+ + ubiquinone + n Na+/in
-
742842
Klebsiella pneumoniae 204
NAD+ + ubiquinol + n Na+/out
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.2.1.1
hexamer
Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon
Klebsiella pneumoniae
7.2.1.1
hexamer
Na+-NQR consists of six subunits (NqrA-F) encoded by six genes of the nqr operon
Vibrio harveyi
General Information
EC Number
General Information
Commentary
Organism
7.2.1.1
malfunction
inactivation of the apbE gene in Klebsiella pneumoniae, wherein the nqr operon and apbE are well separated in the chromosome, results in a complete loss of the quinone reductase activity of Na+-NQR
Klebsiella pneumoniae
7.2.1.1
additional information
the enzyme shows dependence on covalently bound flavin
Klebsiella pneumoniae
7.2.1.1
additional information
the enzyme shows dependence on covalently bound flavin
Vibrio harveyi
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.2.1.1
malfunction
inactivation of the apbE gene in Klebsiella pneumoniae, wherein the nqr operon and apbE are well separated in the chromosome, results in a complete loss of the quinone reductase activity of Na+-NQR
Klebsiella pneumoniae
7.2.1.1
additional information
the enzyme shows dependence on covalently bound flavin
Klebsiella pneumoniae
7.2.1.1
additional information
the enzyme shows dependence on covalently bound flavin
Vibrio harveyi