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Literature summary extracted from

  • Jankowitsch, F.; Kühm, C.; Kellner, R.; Kalinowski, J.; Pelzer, S.; Macheroux, P.; Mack, M.
    A novel N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis (2011), J. Biol. Chem., 286, 38275-38285 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.343 expressed in Escherichia coli Rosetta 2 (DE3) cells Streptomyces davaonensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.343 0.0577
-
 8-amino-8-demethyl-D-riboflavin apparent value, at pH 8.0 and 37°C Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis
-
 S-adenosyl-L-homocysteine + roseoflavin
-
 ?
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis 768
-
 S-adenosyl-L-homocysteine + roseoflavin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.343 Streptomyces davaonensis E7BBN5
-
-
2.1.1.343 Streptomyces davaonensis 768 E7BBN5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.343 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Streptomyces davaonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
 Streptomyces davaonensis S-adenosyl-L-homocysteine + roseoflavin
-
 ?
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
 Streptomyces davaonensis 768 S-adenosyl-L-homocysteine + roseoflavin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.343 ? x * 38000, SDS-PAGE Streptomyces davaonensis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.343 N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase
-
 Streptomyces davaonensis
2.1.1.343 RosA
-
 Streptomyces davaonensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.343 0.37
-
 8-amino-8-demethyl-D-riboflavin at pH 8.0 and 37°C Streptomyces davaonensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.343 S-adenosyl-L-methionine
-
 Streptomyces davaonensis