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Literature summary extracted from
- Structural and biochemical identification of a novel bacterial oxidoreductase (2004), J. Biol. Chem., 279, 50391-50400 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.5.B1 | structures of catalytic subunit YedY in both the molybdenum- and tungsten-substituted forms, five monomers per asymmetric unit | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.B1 | 12 | - |
dimethyl sulfoxide | 25°C, pH not specified in the publication | Escherichia coli |  |
| 1.8.5.B1 | 22 | - |
Trimethylamine N-oxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 27.9 | - |
tetrahydrothiophene oxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 46.4 | - |
Trimethylamine N-oxide | presence of 1 M urea, 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 119 | - |
L-methionine sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.5.B1 | Molybdenum | the molybdenum ion is coordinated by three sulfur ligands, two contributed from the dithiolene sulfurs of the molybdopterin with a Mo-S distance of 2.4 A. The third sulfur ligand is Sgamma of residue C102 at a distance of 2.4 A | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.B1 | Escherichia coli | P76342 and P76343 | P76342 i.e. catalytic subunit YedY/MsrP, P76343 i.e. heme-binding subunit YedZ/MsrQ | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.B1 | dimethyl sulfoxide + reduced benzyl viologen | - |
Escherichia coli | dimethyl sulfide + oxidized benzyl viologen + H2O | - |
? | |
| 1.8.5.B1 | L-methionine sulfoxide + reduced benzyl viologen | - |
Escherichia coli | L-methionine + oxidized benzyl viologen + H2O | - |
? | |
| 1.8.5.B1 | tetrahydrothiophene oxide + reduced benzyl viologen | - |
Escherichia coli | tetrahydrothiophene + oxidized benzyl viologen + H2O | - |
? | |
| 1.8.5.B1 | trimethylamine N-oxide + reduced benzyl viologen | - |
Escherichia coli | trimethylamine + oxidized benzyl viologen + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.B1 | monomer | monomer in solution, light scattering analysis | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.B1 | YedY | - |
Escherichia coli |
| 1.8.5.B1 | YedZ | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.B1 | 4.83 | - |
dimethyl sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 14.8 | - |
Trimethylamine N-oxide | presence of 1 M urea, 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 18.1 | - |
tetrahydrothiophene oxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 19 | - |
Trimethylamine N-oxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 25.6 | - |
L-methionine sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.5.B1 | molybdopterin | presence of one molybdenum molybdopterin not conjugated by an additional nucleotide. | Escherichia coli | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.B1 | 0.215 | - |
L-methionine sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 0.319 | - |
Trimethylamine N-oxide | presence of 1 M urea, 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 0.403 | - |
dimethyl sulfoxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 0.649 | - |
tetrahydrothiophene oxide | 25°C, pH not specified in the publication | Escherichia coli | |
| 1.8.5.B1 | 0.864 | - |
Trimethylamine N-oxide | 25°C, pH not specified in the publication | Escherichia coli | |
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