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Literature summary extracted from

  • Sekine, K.; Moriyama, T.; Kim, J.; Hase, T.; Sato, N.
    Characterization of two ferredoxin-dependent sulfite reductases having different substrate specificity in the red alga Cyanidioschyzon merolae (2017), J. Biochem., 162, 37-43 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.7.1 expression in Escherichia coli Cyanidioschyzon merolae
1.8.7.1 expression in Escherichia coli Cyanidioschyzon merolae

Protein Variants

EC Number Protein Variants Comment Organism
1.7.7.1 G212S/L213T/Y214L/S217C/C220I/S221N mutations mimic partially isoform SiRA Cyanidioschyzon merolae
1.7.7.1 S217C mutation mimics the corresponding residue in isoform SiRA, recovers sulfite reduction activity Cyanidioschyzon merolae
1.8.7.1 G212S/L213T/Y214L/S217C/C220I/S221N mutations mimic partially isoform SiRA Cyanidioschyzon merolae
1.8.7.1 S217C mutation mimics the corresponding residue in isoform SiRA, recovers sulfite reduction activity Cyanidioschyzon merolae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.095
-
 nitrite mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.7.7.1 0.237
-
 nitrite truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.7.7.1 0.395
-
 nitrite truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.8.7.1 0.0064
-
 sulfite mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.8.7.1 0.009
-
 sulfite truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.8.7.1 0.028
-
 sulfite truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Cyanidioschyzon merolae M1URK1 cf. EC 1.8.7.1, isoform SiRA
-
1.7.7.1 Cyanidioschyzon merolae M1V771 cf. EC 1.8.7.1, isoform SiRB
-
1.7.7.1 Cyanidioschyzon merolae 10D M1V771 cf. EC 1.8.7.1, isoform SiRB
-
1.8.7.1 Cyanidioschyzon merolae M1URK1 isoform SiRA
-
1.8.7.1 Cyanidioschyzon merolae M1V771 isoform SiRB
-
1.8.7.1 Cyanidioschyzon merolae 10D M1URK1 isoform SiRA
-
1.8.7.1 Cyanidioschyzon merolae 10D M1V771 isoform SiRB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 additional information enzyme additionally shows activity of sulfite reductase, EC 1.8.7.1 Cyanidioschyzon merolae ?
-
 ?
1.7.7.1 additional information enzyme additionally shows activity of sulfite reductase, EC 1.8.7.1 Cyanidioschyzon merolae 10D ?
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+
-
 Cyanidioschyzon merolae NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + 6 reduced ferredoxin + 7 H+
-
 Cyanidioschyzon merolae 10D NH3 + 2 H2O + 6 oxidized ferredoxin
-
 ?
1.8.7.1 additional information enzyme additionally shows activity of nitrite reductase, EC 1.7.7.1 Cyanidioschyzon merolae ?
-
 ?
1.8.7.1 additional information enzyme additionally shows activity of nitrite reductase, EC 1.7.7.1 Cyanidioschyzon merolae 10D ?
-
 ?
1.8.7.1 sulfite + reduced ferredoxin + H+
-
 Cyanidioschyzon merolae hydrogen sulfide + oxidized ferredoxin + H2O
-
 ?
1.8.7.1 sulfite + reduced ferredoxin + H+
-
 Cyanidioschyzon merolae 10D hydrogen sulfide + oxidized ferredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.7.1 CYME_CMG021C
-
 Cyanidioschyzon merolae
1.7.7.1 CYME_CMJ117C
-
 Cyanidioschyzon merolae
1.7.7.1 SiRA
-
 Cyanidioschyzon merolae
1.7.7.1 SirB
-
 Cyanidioschyzon merolae
1.8.7.1 CYME_CMG021C
-
 Cyanidioschyzon merolae
1.8.7.1 CYME_CMJ117C
-
 Cyanidioschyzon merolae
1.8.7.1 SiRA
-
 Cyanidioschyzon merolae
1.8.7.1 SirB
-
 Cyanidioschyzon merolae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.7.1 1.77
-
 nitrite mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.7.7.1 2.1
-
 nitrite truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.7.7.1 3.77
-
 nitrite truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.8.7.1 0.078
-
 sulfite truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.8.7.1 0.133
-
 sulfite mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae
1.8.7.1 1.93
-
 sulfite truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication Cyanidioschyzon merolae

Expression

EC Number Organism Comment Expression
1.7.7.1 Cyanidioschyzon merolae enzyme is constitutively expressed additional information
1.8.7.1 Cyanidioschyzon merolae enzyme is constitutively expressed additional information