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Literature summary extracted from

  • Figueroa-Teran, R.; Pak, H.; Blomquist, G.J.; Tittiger, C.
    High substrate specificity of ipsdienol dehydrogenase (IDOLDH), a short-chain dehydrogenase from Ips pini bark beetles (2016), J. Biochem., 160, 141-151 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.386 gene IDOLDH, recombinant expression in Spodoptera frugiperda Sf9 cells via baculovirus transfection method Ips pini

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.386 (-)-menthone
-
 Ips pini
1.1.1.386 1,10-phenanthroline
-
 Ips pini
1.1.1.386 1-propanol
-
 Ips pini
1.1.1.386 2-propanol
-
 Ips pini
1.1.1.386 ipsdienone
-
 Ips pini
1.1.1.386 additional information 1,3-butanediol, nerol, geraniol, citral, (S)-ipsdienol do not act as inhibitors Ips pini

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.386 additional information
-
 additional information kinetics, recombinant enzyme Ips pini
1.1.1.386 0.000008
-
 NADP+ pH 7.5, 25°C, recombinant enzyme, with (R)-ipsdienol Ips pini
1.1.1.386 0.002
-
 ipsdienone pH 7.5, 25°C, recombinant enzyme Ips pini
1.1.1.386 0.003
-
 ipsenone pH 7.5, 25°C, recombinant enzyme Ips pini
1.1.1.386 0.009
-
 racemic ipsenol pH 7.5, 25°C, recombinant enzyme Ips pini
1.1.1.386 0.527
-
 (R)-ipsdienol pH 7.5, 25°C, recombinant enzyme Ips pini

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.386 117000
-
 recombinant enzyme, gel filtration Ips pini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.386 (R)-ipsdienol + NADP+ Ips pini stereospecific reaction ipsdienone + NADPH + H+
-
 r
1.1.1.386 racemic ipsenol + NADP+ Ips pini
-
 ipsenone + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.386 Ips pini G5DGA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.386 recombinant enzyme about 2.5fold from Sf9 cells by ammonium sulfate fractionation Ips pini

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.386 additional information IDOLDH expression pattern, overview Ips pini
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.386 0.05
-
 partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsdienone Ips pini
1.1.1.386 0.092
-
 partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsenone Ips pini
1.1.1.386 0.196
-
 partially purified recombinant enzyme, pH 7.5, 25°C, substrate racemic ipsenol Ips pini
1.1.1.386 0.52 0.525 partially purified recombinant enzyme, pH 7.5, 25°C, Tris-HCl or phosphate buffer, substrate (R)-ipsdienol Ips pini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.386 (R)-ipsdienol + NADP+ stereospecific reaction Ips pini ipsdienone + NADPH + H+
-
 r
1.1.1.386 (R)-ipsdienol + NADP+ stereospecific reaction, no activity with (S)-ipsdienol. The oxidation of (R)-ipsdienol is 6.3times higher than that for the reduction of ipsdienone Ips pini ipsdienone + NADPH + H+
-
 r
1.1.1.386 ipsdienone + NADPH + H+ stereospecific reaction Ips pini (R)-ipsdienol + NADP+
-
 r
1.1.1.386 ipsenone + NADPH + H+
-
 Ips pini racemic ipsenol + NADP+
-
 r
1.1.1.386 additional information the enzyme stereospecifically reduces both ketones to their corresponding (R)-alcohols. Low activity with (R)-menthone, 3-penten-2-ol, 17beta-estradiol, poor or no activity with (S)-ipsdienol, nerol, geraniol, myrcene, citral, 4-methyl-3-penten-2-one, 4-methyl-2-pentanone, 4-methyl-2-pentanol, acetone, 1,3-butendiol, etanol, 2-propanol, 3-hydroxybutyrate, and 2-oxobutyrate, substrate specificity, overview. Product identification by GC-MS analysis Ips pini ?
-
 ?
1.1.1.386 racemic ipsenol + NADP+
-
 Ips pini ipsenone + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.386 homotetramer 4 * 27500, recombinant enzyme, SDS-PAGE, functional enzyme form Ips pini

Synonyms

EC Number Synonyms Comment Organism
1.1.1.386 IDOLDH
-
 Ips pini

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.386 25
-
 assay at Ips pini

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.386 7.5
-
 assay at Ips pini

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.386 NADP+
-
 Ips pini
1.1.1.386 NADPH
-
 Ips pini

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.386 0.015
-
 ipsdienone pH 7.5, 25°C, recombinant enzyme Ips pini
1.1.1.386 0.03
-
 (-)-menthone pH 7.5, 25°C, recombinant enzyme Ips pini
1.1.1.386 9.9
-
 2-propanol pH 7.5, 25°C, recombinant enzyme Ips pini
1.1.1.386 33.6
-
 1-propanol pH 7.5, 25°C, recombinant enzyme Ips pini

General Information

EC Number General Information Comment Organism
1.1.1.386 evolution the enzyme belongs to the short-chain oxidoreductase ipsdienol dehydrogenases Ips pini
1.1.1.386 additional information the enzyme contains the typical Rossmann-fold motif for nucleotide binding and the catalytic tetrad that are highly conserved Ips pini
1.1.1.386 physiological function Ips spp. bark beetles use ipsdienol, ipsenol, ipsdienone and ipsenone as aggregation pheromone components and pheromone precursors. For Ips pini, the short-chain oxidoreductase ipsdienol dehydrogenase (IDOLDH) converts (R)-ipsdienol to ipsdienone, and thus likely plays a role in determining pheromone composition. Different IDOLDH orthologues and or activity levels contribute to differences in Ips spp. pheromone composition Ips pini