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Literature summary extracted from

  • Chmelova, D.; Ondrejovic, M.
    Purification and characterization of extracellular laccase produced by Ceriporiopsis subvermispora and decolorization of triphenylmethane dyes (2016), J. Basic Microbiol., 56, 1173-1182 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 EDTA 10 mM, 1.8fold activation Gelatoporia subvermispora
1.10.3.2 SDS 1.6fold activation at 1 mM, 89% inhibition at 10 mM Gelatoporia subvermispora

Application

EC Number Application Comment Organism
1.10.3.2 degradation degradation of synthetic dyes from wastewater using biological treatment Gelatoporia subvermispora
1.10.3.2 environmental protection degradation of synthetic dyes from wastewater using biological treatment Gelatoporia subvermispora

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 K+ 1 mM, 4% inhibition, 10 mM, 15% inhibition Gelatoporia subvermispora
1.10.3.2 Li+ 1 mM, 19% inhibition Gelatoporia subvermispora
1.10.3.2 Na+ 1 mM, 4% inhibition Gelatoporia subvermispora
1.10.3.2 SDS 1.6fold activation at 1 mM, 89% inhibition at 10 mM Gelatoporia subvermispora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.011
-
 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 22°C, pH 5.0 Gelatoporia subvermispora

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Gelatoporia subvermispora
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ba2+ 10 mM, 1.6fold activation Gelatoporia subvermispora
1.10.3.2 Ca2+ 10 mM, 1.2fold activation Gelatoporia subvermispora
1.10.3.2 Cu2+ 10 mM, 8.0fold activation Gelatoporia subvermispora
1.10.3.2 Fe2+ 10 mM, 2.6fold activation Gelatoporia subvermispora
1.10.3.2 Mg2+ 10 mM, 1.5fold activation Gelatoporia subvermispora
1.10.3.2 Mn2+ 10 mM, 2.7fold activation Gelatoporia subvermispora
1.10.3.2 Sn2+ 10 mM, 6.1fold activation Gelatoporia subvermispora
1.10.3.2 Zn2+ 10 mM, 6.4fold activation Gelatoporia subvermispora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 45000
-
 SDS_PAGE Gelatoporia subvermispora

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Gelatoporia subvermispora
-
-
-
1.10.3.2 Gelatoporia subvermispora ATCC90467
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Gelatoporia subvermispora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Gelatoporia subvermispora ?
-
 ?
1.10.3.2 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Gelatoporia subvermispora ATCC90467 ?
-
 ?
1.10.3.2 Bromocresol Purple + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ?
-
 ?
1.10.3.2 Bromocresol Purple + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ATCC90467 ?
-
 ?
1.10.3.2 Malachite Green + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ?
-
 ?
1.10.3.2 Malachite Green + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ATCC90467 ?
-
 ?
1.10.3.2 Methyl Violet + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ?
-
 ?
1.10.3.2 Methyl Violet + O2 the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator Gelatoporia subvermispora ATCC90467 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 45000, SDS-PAGE Gelatoporia subvermispora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Gelatoporia subvermispora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 22
-
 50 h, about 55% loss of activity Gelatoporia subvermispora
1.10.3.2 30
-
 40 h, 70% loss of activity Gelatoporia subvermispora
1.10.3.2 40
-
 80 h, about 50% loss of activity Gelatoporia subvermispora
1.10.3.2 50
-
 20 h, 80% loss of activity Gelatoporia subvermispora
1.10.3.2 60
-
 unstable at Gelatoporia subvermispora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2
-
-
 Gelatoporia subvermispora