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Literature summary extracted from

  • Sah, S.; Aluri, S.; Rex, K.; Varshney, U.
    One-carbon metabolic pathway rewiring in Escherichia coli reveals an evolutionary advantage of 10-formyltetrahydrofolate synthetase (Fhs) in survival under hypoxia (2015), J. Bacteriol., 197, 717-726 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.3 additional information in Escherichia coli DELTAfolD mutants, the essential function of folD can be replaced by Clostridium perfringens fhs when it is provided on a medium-copy-number plasmid or integrated as a single-copy gene in the chromosome. The fhs-supported folD deletion (DELTAfolD) strain grows well in a complex medium. The DELTAfolD strain, harboring heterologous fhs on the chromosome, shows a high NADP+ -to-NADPH ratio and hypersensitivity to trimethoprim. The presence of fhs in Escherichia coli is disadvantageous for its aerobic growth. but under hypoxia, Escherichia coli strains harboring fhs outcompete those lacking it Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Clostridium perfringens
-
 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Clostridium perfringens DSM 756
-
 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Clostridium perfringens Q0TMI3
-
-
6.3.4.3 Clostridium perfringens DSM 756 Q0TMI3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Clostridium perfringens ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Clostridium perfringens DSM 756 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 FHS
-
 Clostridium perfringens
6.3.4.3 Formyltetrahydrofolate synthetase
-
 Clostridium perfringens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.3 ATP
-
 Clostridium perfringens

General Information

EC Number General Information Comment Organism
6.3.4.3 evolution one-carbon metabolic pathway rewiring in Escherichia coli reveals an evolutionary advantage of 10-formyltetrahydrofolate synthetase in survival under hypoxia Clostridium perfringens
6.3.4.3 physiological function N10-formyltetrahydrofolate is required for formylation of eubacterial/organellar initiator tRNA and purine nucleotide biosynthesis. Biosynthesis of N10-formyltetrahydrofolate is catalyzed by 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase (FolD, EC 1.5.1.3) and/or 10-formyltetrahydrofolate synthetase (Fhs) Clostridium perfringens