BRENDA - Enzyme Database

One enzyme, many reactions structural basis for the various reactions catalyzed by naphthalene 1,2-dioxygenase

Ferraro, D.J.; Okerlund, A.; Brown, E.; Ramaswamy, S.; IUCrJ 4, 648-656 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.12.12
expressed in Escherichia coli DE3 Star cells
Pseudomonas sp.
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.14.12.12
hanging drop vapor diffusion method, using 1.9-2.2 M ammonium sulfate, 4-6% (w/v) dioxane, 100 mM MES, pH 5.0-5.8
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.12.12
naphthalene + NADH + H+ + O2
Pseudomonas sp.
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
?
1.14.12.12
naphthalene + NADH + H+ + O2
Pseudomonas sp. C18
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.12.12
Pseudomonas sp.
P0A111
-
-
1.14.12.12
Pseudomonas sp. C18
P0A111
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.12.12
Q Sepharose column chromatography, octyl Sepharose column chromatography, and hydroxyapatite ion-exchange column chromatography
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.12.12
1-chloronaphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
1-chloronaphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
benzamide + NADH + H+ + O2
-
742718
Pseudomonas sp.
?
-
-
-
?
1.14.12.12
benzamide + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
?
-
-
-
?
1.14.12.12
ethylbenzene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
-
-
?
1.14.12.12
ethylbenzene + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
-
-
?
1.14.12.12
ethylphenyl sulfide + NADH + H+ + O2
-
742718
Pseudomonas sp.
(R)-ethylphenyl sulfoxide + NAD+
-
-
-
?
1.14.12.12
ethylphenyl sulfide + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(R)-ethylphenyl sulfoxide + NAD+
-
-
-
?
1.14.12.12
indan + NADH + H+ + O2
-
742718
Pseudomonas sp.
(S)-1-indanol + (R)-1-indanol + NAD+
-
-
-
?
1.14.12.12
indene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(S)-1-indenol + (R)-1-indenol + NAD+
-
-
-
?
1.14.12.12
indole-3-acetate + NADH + H+ + O2
-
742718
Pseudomonas sp.
?
-
-
-
?
1.14.12.12
naphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
naphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
phenetole + NADH + H+ + O2
-
742718
Pseudomonas sp.
phenol + vinyloxybenzene + (S)-ethylphenyl sulfoxide + NAD+
-
-
-
?
1.14.12.12
styrene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(R)-1-phenylethane-1,2-diol + (S)-1-phenylethane-1,2-diol + NAD+
-
-
-
?
1.14.12.12
thioanisole + NADH + H+ + O2
-
742718
Pseudomonas sp.
methyl phenyl sulfoxide + NAD+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.12.12
NADH
-
Pseudomonas sp.
1.14.12.12
[2Fe-2S]-center
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.12.12
expressed in Escherichia coli DE3 Star cells
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.12.12
NADH
-
Pseudomonas sp.
1.14.12.12
[2Fe-2S]-center
-
Pseudomonas sp.
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.14.12.12
hanging drop vapor diffusion method, using 1.9-2.2 M ammonium sulfate, 4-6% (w/v) dioxane, 100 mM MES, pH 5.0-5.8
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.12.12
naphthalene + NADH + H+ + O2
Pseudomonas sp.
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
?
1.14.12.12
naphthalene + NADH + H+ + O2
Pseudomonas sp. C18
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.12.12
Q Sepharose column chromatography, octyl Sepharose column chromatography, and hydroxyapatite ion-exchange column chromatography
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.12.12
1-chloronaphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
1-chloronaphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(1R,2S)-5-chloro-1,2-dihydronaphthalene-1,2-diol + (1S,2R)-8-chloro-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
benzamide + NADH + H+ + O2
-
742718
Pseudomonas sp.
?
-
-
-
?
1.14.12.12
benzamide + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
?
-
-
-
?
1.14.12.12
ethylbenzene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
-
-
?
1.14.12.12
ethylbenzene + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(S)-1-phenylethanol + (R)-1-phenylethanol + NAD+
-
-
-
?
1.14.12.12
ethylphenyl sulfide + NADH + H+ + O2
-
742718
Pseudomonas sp.
(R)-ethylphenyl sulfoxide + NAD+
-
-
-
?
1.14.12.12
ethylphenyl sulfide + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(R)-ethylphenyl sulfoxide + NAD+
-
-
-
?
1.14.12.12
indan + NADH + H+ + O2
-
742718
Pseudomonas sp.
(S)-1-indanol + (R)-1-indanol + NAD+
-
-
-
?
1.14.12.12
indene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(S)-1-indenol + (R)-1-indenol + NAD+
-
-
-
?
1.14.12.12
indole-3-acetate + NADH + H+ + O2
-
742718
Pseudomonas sp.
?
-
-
-
?
1.14.12.12
naphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
naphthalene + NADH + H+ + O2
-
742718
Pseudomonas sp. C18
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
-
-
?
1.14.12.12
phenetole + NADH + H+ + O2
-
742718
Pseudomonas sp.
phenol + vinyloxybenzene + (S)-ethylphenyl sulfoxide + NAD+
-
-
-
?
1.14.12.12
styrene + NADH + H+ + O2
-
742718
Pseudomonas sp.
(R)-1-phenylethane-1,2-diol + (S)-1-phenylethane-1,2-diol + NAD+
-
-
-
?
1.14.12.12
thioanisole + NADH + H+ + O2
-
742718
Pseudomonas sp.
methyl phenyl sulfoxide + NAD+
-
-
-
?