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Literature summary extracted from

  • Guo, X.; Yu, Y.; Liu, X.; Zhang, Y.; Guan, T.; Xie, G.; Wei, J.
    Heterologous expression and characterization of human cellular glutathione peroxidase mutants (2014), IUBMB Life, 66, 212-219 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.9 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.9 C2S/C78S/C115S/C156S/C202S about 10fold increase in activity compared to wild-type. Mutant displays a typical ping-pong mechanism Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.9 0.02
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C Homo sapiens
1.11.1.9 0.022
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C Homo sapiens
1.11.1.9 0.31
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C Homo sapiens
1.11.1.9 0.31
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.9 selenium selenium-dependent enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.9 Homo sapiens P07203
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.9 Hep-G2 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.9 0.52
-
 wild-type, pH 7.4, 37°C Homo sapiens
1.11.1.9 5.28
-
 mutant C2S/C78S/C115S/C156S/C202S, pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.9 2 glutathione + H2O2
-
 Homo sapiens glutathione disulfide + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.9 GPX1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.9 37
-
 mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.9 50
-
 75% of maximum activity, mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.9 4.7
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C Homo sapiens
1.11.1.9 4.8
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C Homo sapiens
1.11.1.9 683
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C Homo sapiens
1.11.1.9 727.8
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.9 8.5
-
 wild-type Homo sapiens
1.11.1.9 9
-
 mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.9 7.4
-
 26% of maximum activity, mutant C2S/C78S/C115S/C156S/C202S Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.9 216.7
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C Homo sapiens
1.11.1.9 216.7
-
 glutathione mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C Homo sapiens
1.11.1.9 231.7
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C Homo sapiens
1.11.1.9 231.7
-
 H2O2 mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C Homo sapiens