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Literature summary extracted from

  • Mahdizadehdehosta, R.; Kianmehr, A.; Khalili, A.
    Isolation and characterization of leucine dehydrogenase from a thermophilic Citrobacter freundii JK-91 strain isolated from Jask Port (2013), Iran. J. Microbiol., 5, 278-284 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 additional information not inhibitory: EDTA, Ca2+, Mg2+ Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 1.2
-
 L-leucine pH not specified in the publication, temperature not specified in the publication Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 320000
-
 gel filtration Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Citrobacter freundii
-
-
-
1.4.1.9 Citrobacter freundii JK-91
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
 Citrobacter freundii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 320
-
 pH not specified in the publication, temperature not specified in the publication Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-leucine + H2O + NAD+
-
 Citrobacter freundii 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-leucine + H2O + NAD+
-
 Citrobacter freundii JK-91 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 additional information no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine Citrobacter freundii ?
-
 ?
1.4.1.9 additional information no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine Citrobacter freundii JK-91 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.9 octamer 8 * 40000, SDS-PAGE Citrobacter freundii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.9 60
-
-
 Citrobacter freundii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.9 60 75
-
 Citrobacter freundii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 10.5
-
-
 Citrobacter freundii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.9 9 11
-
 Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 additional information no cofactor: NADP+, NADPH Citrobacter freundii
1.4.1.9 NAD+
-
 Citrobacter freundii
1.4.1.9 NADH
-
 Citrobacter freundii