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Literature summary extracted from

  • Zhou, T.S.; Zhou, R.; Yu, Y.B.; Xiao, Y.; Li, D.H.; Xiao, B.; Yu, O.; Yang, Y.J.
    Cloning and characterization of a flavonoid 3-hydroxylase gene from tea plant (Camellia sinensis) (2016), Int. J. Mol. Sci., 17, 261 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.82 expression in Saccharomyces cerevisiae WAT11 Camellia sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.82 0.0171
-
 naringenin pH 7.0, 30°C Camellia sinensis
1.14.14.82 0.06806
-
 kaempferol pH 7.0, 30°C Camellia sinensis
1.14.14.82 0.1436
-
 dihydrokaempferol pH 7.0, 30°C Camellia sinensis

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.82 Camellia sinensis A0A0S2LLA9 cv. Wuniuzao
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.82 leaf
-
 Camellia sinensis
-
1.14.14.82 shoot
-
 Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Camellia sinensis dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Camellia sinensis quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2 optimal substrate Camellia sinensis eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.82 CsF3'H
-
 Camellia sinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.82 0.00000016
-
 dihydrokaempferol pH 7.0, 30°C Camellia sinensis
1.14.14.82 0.00000036
-
 kaempferol pH 7.0, 30°C Camellia sinensis
1.14.14.82 0.00000082
-
 naringenin pH 7.0, 30°C Camellia sinensis

Expression

EC Number Organism Comment Expression
1.14.14.82 Camellia sinensis expression level increases in response to nitrogen depletion after 12 days of treatment, in agreement with a corresponding increase in 3',4'-catechins, 3',4',5'-catechins and flavan 3-ols content in the leaves up

General Information

EC Number General Information Comment Organism
1.14.14.82 metabolism the enzyme is involved in biosynthesis of 3',4'-catechins, 3',4',5'-catechins and flavan 3-ols in the leaves Camellia sinensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.82 0.0000011
-
 dihydrokaempferol pH 7.0, 30°C Camellia sinensis
1.14.14.82 0.0000053
-
 kaempferol pH 7.0, 30°C Camellia sinensis
1.14.14.82 0.000048
-
 naringenin pH 7.0, 30°C Camellia sinensis