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Literature summary extracted from

  • Meng, J.; Yang, Y.; Xiao, C.; Guan, Y.; Hao, X.; Deng, Q.; Lu, Z.
    Identification and validation of aspartic acid semialdehyde dehydrogenase as a new anti-Mycobacterium tuberculosis target (2015), Int. J. Mol. Sci., 16, 23572-23586 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.11 additional information construction of an gene asadh promoter-replacement mutant Mycobacterium tuberculosis strain, designated MTB::asadh, in which the asadh gene expression is regulated by pristinamycin. Bacterial growth is survival of MTB::asadh in host cells is completely inhibited in the absence of the inducer pristinamycin. The growth of the mutant is rigorously dependent on the presence of the inducer in the medium. The starved mutant exhibits a marked reduction (approximately 80%) in the cell wall materials compared to the wild-type, in addition to obvious morphological differences, phenotype, overview. With the addition of pristinamycin, the cell wall contents and morphology are recovered in a similar manner to those of the wild-type strain. The starved mutant also exhibits almost no pathogenicity in an in vitro model of infection using mouse macrophage J774A.1 cells. The mutant shows a concentration-dependent recovery of pathogenicity with the addition of the inducer Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis
-
 L-4-aspartyl phosphate + NADPH + H+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Mycobacterium tuberculosis H37Rv
-
 L-4-aspartyl phosphate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Mycobacterium tuberculosis
-
-
-
1.2.1.11 Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Mycobacterium tuberculosis L-4-aspartyl phosphate + NADPH + H+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Mycobacterium tuberculosis H37Rv L-4-aspartyl phosphate + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Mycobacterium tuberculosis
1.2.1.11 aspartic acid semialdehyde dehydrogenase
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Mycobacterium tuberculosis
1.2.1.11 NADPH
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.2.1.11 malfunction enzyme deficiency or inhibition of enzyme activity leads to 80% reduced cell wall materials compared to the wild-type, in addition to obvious morphological differences, phenotype, overview Mycobacterium tuberculosis