Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ken, C.F.; Huang, C.Y.; Wen, L.; Huang, J.K.; Lin, C.T.
    Modulation of nitrosative stress via glutathione-dependent formaldehyde dehydrogenase and S-nitrosoglutathione reductase (2014), Int. J. Mol. Sci., 15, 14166-14179 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.284 glutathione dependent on Taiwanofungus camphoratus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.284 recombinant expression of wild-type and mutant enzymes in HEK 293T cells, transfectioon via a v-raf/v-myc oncogene-carrying retrovirus, improvement by codon optimization. The Tc-GFD protein expressed in pcGFD-O transfected 293T cells provides protection to these cells against apoptosis under GSNO induced oxidative stress, overview Taiwanofungus camphoratus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.284 C47S site-directed mutagenesis, active site mutant Taiwanofungus camphoratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.284 S-(hydroxymethyl)glutathione + NAD+ Taiwanofungus camphoratus
-
 S-formylglutathione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Taiwanofungus camphoratus D2CFJ2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 S-(hydroxymethyl)glutathione + NAD+
-
 Taiwanofungus camphoratus S-formylglutathione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 GFD
-
 Taiwanofungus camphoratus
1.1.1.284 Glutathione-dependent formaldehyde dehydrogenase
-
 Taiwanofungus camphoratus
1.1.1.284 Tc-GFD formerly named Ac-GFD Taiwanofungus camphoratus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Taiwanofungus camphoratus

General Information

EC Number General Information Comment Organism
1.1.1.284 physiological function glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. Formaldehyde detoxification involves the conversion of formaldehyde to formate, which consists of three reactions: The first reaction is a spontaneous condensation reaction between formaldehyde and glutathione producing S-hydroxymethylglutathione (HMGSH). The second reaction, catalyzed by enzyme GFD, is the oxidation of HMGSH to form S-formylglutathione. In the third reaction, catalyzed by S-formylglutathione hydrolase (FGH), formate is formed and glutathione regenerated Taiwanofungus camphoratus