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Literature summary extracted from

  • Kim, E.; Tong, X.; Lee, J.
    The ferredoxin Rr-HydB is required for the H2-evolving activity of Rr-HydA, a [FeFe]-hydrogenase of Rhodospirillum rubrum (2015), Int. J. Hydrogen Energy, 40, 4320-4328 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.7.2 expressed in Escherichia coli BL21 CodonPlus(DE3) cells and in a Rhodobacter sphaeroides nifDK hupSL mutant Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Rhodospirillum rubrum
-
reduced ferredoxin + H+
-
?
1.12.7.2 H2 + oxidized ferredoxin Rhodospirillum rubrum UR1
-
reduced ferredoxin + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Rhodospirillum rubrum
-
-
-
1.12.7.2 Rhodospirillum rubrum UR1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 Strep-Tactin-Sepharose column chromatography and Superdex 200 gel filtration Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + methyl viologen
-
Rhodospirillum rubrum reduced methyl viologen + H+
-
?
1.12.7.2 H2 + methyl viologen
-
Rhodospirillum rubrum UR1 reduced methyl viologen + H+
-
?
1.12.7.2 H2 + oxidized ferredoxin
-
Rhodospirillum rubrum reduced ferredoxin + H+
-
?
1.12.7.2 H2 + oxidized ferredoxin
-
Rhodospirillum rubrum UR1 reduced ferredoxin + H+
-
?
1.12.7.2 H2 + oxidized spinach ferredoxin
-
Rhodospirillum rubrum reduced spinach ferredoxin + H+
-
?
1.12.7.2 H2 + oxidized spinach ferredoxin
-
Rhodospirillum rubrum UR1 reduced spinach ferredoxin + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 ? x * 50000, SDS-PAGE Rhodospirillum rubrum

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 hydA
-
Rhodospirillum rubrum
1.12.7.2 [FeFe]-hydrogenase
-
Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.7.2 [Fe-S]-center
-
Rhodospirillum rubrum