BRENDA - Enzyme Database show

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Rekik, H.; Nadia, Z.J.; Bejar, W.; Kourdali, S.; Belhoul, M.; Hmidi, M.; Benkiar, A.; Badis, A.; Sallem, N.; Bejar, S.; Jaouadi, B.; Int. J. Biol. Macromol. 73, 253-263 (2015)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.14
sodium carbonate
50 mM, 125% of initial activity
Streptomyces griseosporeus
1.11.1.14
tetraacetylethylenediamine
25 mM, 121% of initial activity
Streptomyces griseosporeus
1.11.1.14
Triton X-100
5% w/v, 123% of initial activity
Streptomyces griseosporeus
Application
EC Number
Application
Commentary
Organism
1.11.1.14
degradation
enzyme shows marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40C
Streptomyces griseosporeus
General Stability
EC Number
General Stability
Organism
1.11.1.19
the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively.
Streptomyces griseosporeus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.14
SDS
1% w/v, 72% residual activity
Streptomyces griseosporeus
1.11.1.14
sodium perborate
5% w/v, 71% residual activity
Streptomyces griseosporeus
1.11.1.14
Urea
1% w/v, 85% residual activity
Streptomyces griseosporeus
1.11.1.19
Ba2+
about 85% inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Cd2+
complete inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Co2+
about 56% inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Hg2+
complete inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
potassium cyanide
13% inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Sodium azide
9% inhibition at 1 mM
Streptomyces griseosporeus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.11
-
2,4-Dichlorophenol
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.14
0.46
-
o-Dianisidine
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.19
0.112
-
2,4-Dichlorophenol
at pH 8.5 and 25C
Streptomyces griseosporeus
1.11.1.19
0.458
-
o-Dianisidine
at pH 8.5 and 25C
Streptomyces griseosporeus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.14
extracellular
-
Streptomyces griseosporeus
-
-
1.11.1.19
extracellular
-
Streptomyces griseosporeus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.19
Ca2+
about 116% activity at 1 mM
Streptomyces griseosporeus
1.11.1.19
Mn2+
about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme
Streptomyces griseosporeus
1.11.1.19
additional information
Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity
Streptomyces griseosporeus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.14
Streptomyces griseosporeus
-
-
-
1.11.1.14
Streptomyces griseosporeus SN9
-
-
-
1.11.1.19
Streptomyces griseosporeus
-
-
-
1.11.1.19
Streptomyces griseosporeus SN9
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.14
purification from culture supernatant
Streptomyces griseosporeus
1.11.1.19
OH-40 column chromatography and UNO Q-6 column chromatography
Streptomyces griseosporeus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.11.1.14
5.75
-
substrate 2,4-dichlorophenol, pH 8.5, temperature not specified in the publication
Streptomyces griseosporeus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.14
2 guaiacol + H2O2
27% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
-
-
?
1.11.1.14
2 guaiacol + H2O2
27% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
-
-
?
1.11.1.14
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
92% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
92% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.14
2,4,6-trichlorophenol + H2O2
63% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
2,4,6-trichlorophenol + H2O2
63% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.14
2,4-dichlorophenol + H2O2
best substrate
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
4-chlorophenol + H2O2
45% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
humic acid + H2O2
-
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
humic acid + H2O2
-
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.14
o-dianisidine + H2O2
76% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
o-dianisidine + H2O2
76% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.19
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
about 92% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
2,4,6-trichlorophenol + H2O2 + H+
about 63% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
2,4-dichlorophenol + H2O2 + H+
100% activity
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
2,6-dimethoxyphenol + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
4-trichlorophenol + H2O2 + H+
about 45% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
Acid Blue 158 + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
Cibacet Brilliant Blue BG + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
guaiacol + H2O2 + H+
about 27% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
additional information
no activity with commercial humic acid
742684
Streptomyces griseosporeus
?
-
-
-
-
1.11.1.19
o-dianisidine + H2O2 + H+
about 76% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
pyrogallol + H2O2 + H+
about 75% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
Remazol Brilliant Blue Reactif + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.11.1.14
?
x * 43000, SDS-PAGE
Streptomyces griseosporeus
1.11.1.19
monomer
1 * 43000, SDS-PAGE
Streptomyces griseosporeus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.14
60
-
-
Streptomyces griseosporeus
1.11.1.19
65
-
-
Streptomyces griseosporeus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.11.1.19
60
-
the half-life at 60C is 120 min
Streptomyces griseosporeus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.06
-
2,4-Dichlorophenol
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.14
1.16
-
o-Dianisidine
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.19
0.0635
-
2,4-Dichlorophenol
at pH 8.5 and 25C
Streptomyces griseosporeus
1.11.1.19
1.17
-
o-Dianisidine
at pH 8.5 and 25C
Streptomyces griseosporeus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.14
8.5
-
-
Streptomyces griseosporeus
1.11.1.19
8.5
-
-
Streptomyces griseosporeus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.11.1.19
7
10
the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40C
Streptomyces griseosporeus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.14
heme
spectrum shows a heme peak at 409 nm
Streptomyces griseosporeus
1.11.1.19
heme
-
Streptomyces griseosporeus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.14
sodium carbonate
50 mM, 125% of initial activity
Streptomyces griseosporeus
1.11.1.14
tetraacetylethylenediamine
25 mM, 121% of initial activity
Streptomyces griseosporeus
1.11.1.14
Triton X-100
5% w/v, 123% of initial activity
Streptomyces griseosporeus
Application (protein specific)
EC Number
Application
Commentary
Organism
1.11.1.14
degradation
enzyme shows marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40C
Streptomyces griseosporeus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.14
heme
spectrum shows a heme peak at 409 nm
Streptomyces griseosporeus
1.11.1.19
heme
-
Streptomyces griseosporeus
General Stability (protein specific)
EC Number
General Stability
Organism
1.11.1.19
the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively.
Streptomyces griseosporeus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.14
SDS
1% w/v, 72% residual activity
Streptomyces griseosporeus
1.11.1.14
sodium perborate
5% w/v, 71% residual activity
Streptomyces griseosporeus
1.11.1.14
Urea
1% w/v, 85% residual activity
Streptomyces griseosporeus
1.11.1.19
Ba2+
about 85% inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Cd2+
complete inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Co2+
about 56% inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Hg2+
complete inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
potassium cyanide
13% inhibition at 1 mM
Streptomyces griseosporeus
1.11.1.19
Sodium azide
9% inhibition at 1 mM
Streptomyces griseosporeus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.11
-
2,4-Dichlorophenol
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.14
0.46
-
o-Dianisidine
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.19
0.112
-
2,4-Dichlorophenol
at pH 8.5 and 25C
Streptomyces griseosporeus
1.11.1.19
0.458
-
o-Dianisidine
at pH 8.5 and 25C
Streptomyces griseosporeus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.14
extracellular
-
Streptomyces griseosporeus
-
-
1.11.1.19
extracellular
-
Streptomyces griseosporeus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.19
Ca2+
about 116% activity at 1 mM
Streptomyces griseosporeus
1.11.1.19
Mn2+
about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme
Streptomyces griseosporeus
1.11.1.19
additional information
Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity
Streptomyces griseosporeus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.14
purification from culture supernatant
Streptomyces griseosporeus
1.11.1.19
OH-40 column chromatography and UNO Q-6 column chromatography
Streptomyces griseosporeus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.11.1.14
5.75
-
substrate 2,4-dichlorophenol, pH 8.5, temperature not specified in the publication
Streptomyces griseosporeus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.14
2 guaiacol + H2O2
27% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
-
-
?
1.11.1.14
2 guaiacol + H2O2
27% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
-
-
?
1.11.1.14
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
92% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
92% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.14
2,4,6-trichlorophenol + H2O2
63% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
2,4,6-trichlorophenol + H2O2
63% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.14
2,4-dichlorophenol + H2O2
best substrate
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
4-chlorophenol + H2O2
45% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
humic acid + H2O2
-
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
humic acid + H2O2
-
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.14
o-dianisidine + H2O2
76% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus
? + H2O
-
-
-
?
1.11.1.14
o-dianisidine + H2O2
76% of the activity with 2,4-dichlorophenol
742684
Streptomyces griseosporeus SN9
? + H2O
-
-
-
?
1.11.1.19
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
about 92% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
2,4,6-trichlorophenol + H2O2 + H+
about 63% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
2,4-dichlorophenol + H2O2 + H+
100% activity
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
2,6-dimethoxyphenol + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
4-trichlorophenol + H2O2 + H+
about 45% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
Acid Blue 158 + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
Cibacet Brilliant Blue BG + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
guaiacol + H2O2 + H+
about 27% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
additional information
no activity with commercial humic acid
742684
Streptomyces griseosporeus
?
-
-
-
-
1.11.1.19
o-dianisidine + H2O2 + H+
about 76% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
pyrogallol + H2O2 + H+
about 75% activity compared to 2,4-dichlorophenol
742684
Streptomyces griseosporeus
?
-
-
-
?
1.11.1.19
Remazol Brilliant Blue Reactif + H2O2 + H+
-
742684
Streptomyces griseosporeus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.1.14
?
x * 43000, SDS-PAGE
Streptomyces griseosporeus
1.11.1.19
monomer
1 * 43000, SDS-PAGE
Streptomyces griseosporeus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.14
60
-
-
Streptomyces griseosporeus
1.11.1.19
65
-
-
Streptomyces griseosporeus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.11.1.19
60
-
the half-life at 60C is 120 min
Streptomyces griseosporeus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.06
-
2,4-Dichlorophenol
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.14
1.16
-
o-Dianisidine
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.19
0.0635
-
2,4-Dichlorophenol
at pH 8.5 and 25C
Streptomyces griseosporeus
1.11.1.19
1.17
-
o-Dianisidine
at pH 8.5 and 25C
Streptomyces griseosporeus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.14
8.5
-
-
Streptomyces griseosporeus
1.11.1.19
8.5
-
-
Streptomyces griseosporeus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.11.1.19
7
10
the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40C
Streptomyces griseosporeus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.57
-
2,4-Dichlorophenol
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.14
2.53
-
o-Dianisidine
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.19
0.567
-
2,4-Dichlorophenol
at pH 8.5 and 25C
Streptomyces griseosporeus
1.11.1.19
2.54
-
o-Dianisidine
at pH 8.5 and 25C
Streptomyces griseosporeus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.11.1.14
0.57
-
2,4-Dichlorophenol
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.14
2.53
-
o-Dianisidine
pH 8.5, 60C
Streptomyces griseosporeus
1.11.1.19
0.567
-
2,4-Dichlorophenol
at pH 8.5 and 25C
Streptomyces griseosporeus
1.11.1.19
2.54
-
o-Dianisidine
at pH 8.5 and 25C
Streptomyces griseosporeus