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Literature summary extracted from

  • Rekik, H.; Nadia, Z.J.; Bejar, W.; Kourdali, S.; Belhoul, M.; Hmidi, M.; Benkiar, A.; Badis, A.; Sallem, N.; Bejar, S.; Jaouadi, B.
    Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9 (2015), Int. J. Biol. Macromol., 73, 253-263 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.14 sodium carbonate 50 mM, 125% of initial activity Streptomyces griseosporeus
1.11.1.14 tetraacetylethylenediamine 25 mM, 121% of initial activity Streptomyces griseosporeus
1.11.1.14 Triton X-100 5% w/v, 123% of initial activity Streptomyces griseosporeus

Application

EC Number Application Comment Organism
1.11.1.14 degradation enzyme shows marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C Streptomyces griseosporeus

General Stability

EC Number General Stability Organism
1.11.1.19 the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively. Streptomyces griseosporeus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.14 SDS 1% w/v, 72% residual activity Streptomyces griseosporeus
1.11.1.14 sodium perborate 5% w/v, 71% residual activity Streptomyces griseosporeus
1.11.1.14 Urea 1% w/v, 85% residual activity Streptomyces griseosporeus
1.11.1.19 Ba2+ about 85% inhibition at 1 mM Streptomyces griseosporeus
1.11.1.19 Cd2+ complete inhibition at 1 mM Streptomyces griseosporeus
1.11.1.19 Co2+ about 56% inhibition at 1 mM Streptomyces griseosporeus
1.11.1.19 Hg2+ complete inhibition at 1 mM Streptomyces griseosporeus
1.11.1.19 potassium cyanide 13% inhibition at 1 mM Streptomyces griseosporeus
1.11.1.19 Sodium azide 9% inhibition at 1 mM Streptomyces griseosporeus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.14 0.11
-
 2,4-Dichlorophenol pH 8.5, 60°C Streptomyces griseosporeus
1.11.1.14 0.46
-
 o-Dianisidine pH 8.5, 60°C Streptomyces griseosporeus
1.11.1.19 0.112
-
 2,4-Dichlorophenol at pH 8.5 and 25°C Streptomyces griseosporeus
1.11.1.19 0.458
-
 o-Dianisidine at pH 8.5 and 25°C Streptomyces griseosporeus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.14 extracellular
-
 Streptomyces griseosporeus
-
-
1.11.1.19 extracellular
-
 Streptomyces griseosporeus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.19 Ca2+ about 116% activity at 1 mM Streptomyces griseosporeus
1.11.1.19 Mn2+ about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme Streptomyces griseosporeus
1.11.1.19 additional information Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity Streptomyces griseosporeus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.14 Streptomyces griseosporeus
-
-
-
1.11.1.14 Streptomyces griseosporeus SN9
-
-
-
1.11.1.19 Streptomyces griseosporeus
-
-
-
1.11.1.19 Streptomyces griseosporeus SN9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.14 purification from culture supernatant Streptomyces griseosporeus
1.11.1.19 OH-40 column chromatography and UNO Q-6 column chromatography Streptomyces griseosporeus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.14 5.75
-
 substrate 2,4-dichlorophenol, pH 8.5, temperature not specified in the publication Streptomyces griseosporeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.14 2 guaiacol + H2O2 27% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
1.11.1.14 2 guaiacol + H2O2 27% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
1.11.1.14 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 92% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
1.11.1.14 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 92% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 ? + H2O
-
 ?
1.11.1.14 2,4,6-trichlorophenol + H2O2 63% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
1.11.1.14 2,4,6-trichlorophenol + H2O2 63% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 ? + H2O
-
 ?
1.11.1.14 2,4-dichlorophenol + H2O2 best substrate Streptomyces griseosporeus ? + H2O
-
 ?
1.11.1.14 4-chlorophenol + H2O2 45% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
1.11.1.14 humic acid + H2O2
-
 Streptomyces griseosporeus ? + H2O
-
 ?
1.11.1.14 humic acid + H2O2
-
 Streptomyces griseosporeus SN9 ? + H2O
-
 ?
1.11.1.14 o-dianisidine + H2O2 76% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus ? + H2O
-
 ?
1.11.1.14 o-dianisidine + H2O2 76% of the activity with 2,4-dichlorophenol Streptomyces griseosporeus SN9 ? + H2O
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 about 92% activity compared to 2,4-dichlorophenol Streptomyces griseosporeus ?
-
 ?
1.11.1.19 2,4,6-trichlorophenol + H2O2 + H+ about 63% activity compared to 2,4-dichlorophenol Streptomyces griseosporeus ?
-
 ?
1.11.1.19 2,4-dichlorophenol + H2O2 + H+ 100% activity Streptomyces griseosporeus ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces griseosporeus ?
-
 ?
1.11.1.19 4-chlorophenol + H2O2 + H+ about 45% activity compared to 2,4-dichlorophenol Streptomyces griseosporeus ?
-
 ?
1.11.1.19 Acid Blue 158 + H2O2 + H+
-
 Streptomyces griseosporeus ?
-
 ?
1.11.1.19 Cibacet Brilliant Blue BG + H2O2 + H+
-
 Streptomyces griseosporeus ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ about 27% activity compared to 2,4-dichlorophenol Streptomyces griseosporeus ?
-
 ?
1.11.1.19 additional information no activity with commercial humic acid Streptomyces griseosporeus ?
-
 ?
1.11.1.19 o-dianisidine + H2O2 + H+ about 76% activity compared to 2,4-dichlorophenol Streptomyces griseosporeus ?
-
 ?
1.11.1.19 pyrogallol + H2O2 + H+ about 75% activity compared to 2,4-dichlorophenol Streptomyces griseosporeus ?
-
 ?
1.11.1.19 Remazol Brilliant Blue Reactif + H2O2 + H+
-
 Streptomyces griseosporeus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.14 ? x * 43000, SDS-PAGE Streptomyces griseosporeus
1.11.1.19 monomer 1 * 43000, SDS-PAGE Streptomyces griseosporeus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 LiP-SN
-
 Streptomyces griseosporeus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.14 60
-
-
 Streptomyces griseosporeus
1.11.1.19 65
-
-
 Streptomyces griseosporeus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.19 60
-
 the half-life at 60°C is 120 min Streptomyces griseosporeus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.14 0.06
-
 2,4-Dichlorophenol pH 8.5, 60°C Streptomyces griseosporeus
1.11.1.14 1.16
-
 o-Dianisidine pH 8.5, 60°C Streptomyces griseosporeus
1.11.1.19 0.0635
-
 2,4-Dichlorophenol at pH 8.5 and 25°C Streptomyces griseosporeus
1.11.1.19 1.17
-
 o-Dianisidine at pH 8.5 and 25°C Streptomyces griseosporeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.14 8.5
-
-
 Streptomyces griseosporeus
1.11.1.19 8.5
-
-
 Streptomyces griseosporeus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.19 7 10 the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40°C Streptomyces griseosporeus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.14 heme spectrum shows a heme peak at 409 nm Streptomyces griseosporeus
1.11.1.19 heme
-
 Streptomyces griseosporeus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.14 0.57
-
 2,4-Dichlorophenol pH 8.5, 60°C Streptomyces griseosporeus
1.11.1.14 2.53
-
 o-Dianisidine pH 8.5, 60°C Streptomyces griseosporeus
1.11.1.19 0.567
-
 2,4-Dichlorophenol at pH 8.5 and 25°C Streptomyces griseosporeus
1.11.1.19 2.54
-
 o-Dianisidine at pH 8.5 and 25°C Streptomyces griseosporeus