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Literature summary extracted from

  • Romdhan, I.B.; Fendri, A.; Frikha, F.; Gargouri, A.; Belghith, H.
    Purification, physico-chemical and kinetic properties of the deglycosylated Talaromyces thermophilus lipase (2012), Int. J. Biol. Macromol., 51, 892-900 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Thermomyces dupontii F6LQK7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein treatment with endoglycosidase H decreases molecular mass from 39000 Da to 30000 Da Thermomyces dupontii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Thermomyces dupontii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 2100
-
 deglycosylated form, substrate glyceryl tributanoate, 45°C, pH 9.5 Thermomyces dupontii
3.1.1.3 4642
-
 deglycosylated form, substrate olive oil emulsion, 45°C, pH 9.5 Thermomyces dupontii
3.1.1.3 9608
-
 glycosylated form, substrate olive oil emulsion, 50°C, pH 9.5 Thermomyces dupontii
3.1.1.3 12000
-
 deglycosylated form, substrate glyceryl trioctanoate, 45°C, pH 9.5 Thermomyces dupontii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 glyceryl tributanoate + H2O
-
 Thermomyces dupontii glycerol + butanoate
-
 ?
3.1.1.3 glyceryl trioctanoate + H2O
-
 Thermomyces dupontii glycerol + octanoate
-
 ?
3.1.1.3 olive oil + H2O
-
 Thermomyces dupontii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 30000, deglycosylated form, x * 39000, glycosylated form, SDS-PAGE, x * 29590, calculated Thermomyces dupontii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 TTL
-
 Thermomyces dupontii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 45
-
 deglycosylated form Thermomyces dupontii
3.1.1.3 50
-
 glycosylated form Thermomyces dupontii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9.5
-
-
 Thermomyces dupontii

General Information

EC Number General Information Comment Organism
3.1.1.3 physiological function deglycosylation of the enzyme reduces its specific activity by 50% Thermomyces dupontii