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Literature summary extracted from

  • Bouacem, K.; Rekik, H.; Jaouadi, N.Z.; Zenati, B.; Kourdali, S.; El Hattab, M.; Badis, A.; Annane, R.; Bejar, S.; Hacene, H.; Bouanane-Darenfed, A.; Jaouadi, B.
    Purification and characterization of two novel peroxidases from the dye-decolorizing fungus Bjerkandera adusta strain CX-9 (2017), Int. J. Biol. Macromol., 106, 636-646 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.19 H2O2 the maximum activity is obtained with an initial H2O2 concentration of about 0.33 mM Bjerkandera adusta

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.19 2-mercaptoethanol 55% residual activity at 1 mM; 70% residual activity at 1 mM Bjerkandera adusta
1.11.1.19 Ag+ 50% residual activity at 5 mM; 82% residual activity at 5 mM Bjerkandera adusta
1.11.1.19 Ba2+ 90% residual activity at 5 mM Bjerkandera adusta
1.11.1.19 Cd2+ complete inhibition at 5 mM Bjerkandera adusta
1.11.1.19 Co2+ 66% residual activity at 5 mM Bjerkandera adusta
1.11.1.19 dithiothreitol 65% residual activity at 1 mM; 81% residual activity at 1 mM Bjerkandera adusta
1.11.1.19 Fe2+ 85% residual activity at 5 mM Bjerkandera adusta
1.11.1.19 Hg2+ complete inhibition at 5 mM Bjerkandera adusta
1.11.1.19 hydroxylamine 10% residual activity at 2 mM; 25% residual activity at 2 mM Bjerkandera adusta
1.11.1.19 L-cystine 10% residual activity at 2 mM; 25% residual activity at 2 mM Bjerkandera adusta
1.11.1.19 potassium cyanide 3% residual activity at 10 mM; complete inhibition at 10 mM Bjerkandera adusta
1.11.1.19 SDS 27% inhibition at 20 mM; 68% residual activity at 20 mM; 73% residual activity at 20 mM Bjerkandera adusta
1.11.1.19 Sodium azide 5% residual activity at 10 mM; complete inhibition at 10 mM Bjerkandera adusta
1.11.1.19 Zn2+ 88% residual activity at 5 mM Bjerkandera adusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.099
-
 2,4-Dichlorophenol at pH 4.0 and 30°C Bjerkandera adusta
1.11.1.19 0.099
-
 2,4-Dichlorophenol at pH 3.0 and 70°C Bjerkandera adusta
1.11.1.19 0.151
-
 2,6-dimethoxyphenol at pH 4.0 and 50°C Bjerkandera adusta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.19 Ca2+ 118% activity at 5 mM Bjerkandera adusta
1.11.1.19 Ca2+ 110% activity at 5 mM Bjerkandera adusta
1.11.1.19 Co2+ 120% activity at 5 mM Bjerkandera adusta
1.11.1.19 Cu2+ 114% activity at 5 mM Bjerkandera adusta
1.11.1.19 Cu2+ 133% activity at 5 mM Bjerkandera adusta
1.11.1.19 Fe2+ 109% activity at 5 mM Bjerkandera adusta
1.11.1.19 K+ 125% activity at 5 mM Bjerkandera adusta
1.11.1.19 Mg2+ 180% activity at 5 mM Bjerkandera adusta
1.11.1.19 Mg2+ 180% activity at 5 mM. The addition of 5 mM of Mg2+ enhances the thermostability of the enzyme Bjerkandera adusta
1.11.1.19 Mn2+ 112% activity at 5 mM Bjerkandera adusta
1.11.1.19 Mn2+ 225% activity at 5 mM. The addition of 5 mM of Mn2+ enhances the thermostability of the enzyme Bjerkandera adusta
1.11.1.19 additional information not significantly affected by Mg2+, Ba2+, Li+, and K+ Bjerkandera adusta
1.11.1.19 additional information not significantly affected by Zn2+, Fe2+, and Li+ Bjerkandera adusta
1.11.1.19 Zn2+ 104% activity at 5 mM Bjerkandera adusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.19 Reactive Blue 5 + H2O2 Bjerkandera adusta
-
 phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.19 hexane the enzyme retains at least 22% of the activity of incubation in the presence of 30% (v/v) hexane Bjerkandera adusta
1.11.1.19 hexane the enzyme retains at least 75% of the activity of incubation in the presence of 30% (v/v) hexane Bjerkandera adusta
1.11.1.19 isopropanol the enzyme retains at least 115% of the activity of incubation in the presence of 30% (v/v) isopropanol Bjerkandera adusta
1.11.1.19 isopropanol the enzyme retains at least 95% of the activity of incubation in the presence of 30% (v/v) isopropanol Bjerkandera adusta

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Bjerkandera adusta
-
-
-
1.11.1.19 Bjerkandera adusta CX-9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.19 ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange column chromatography, and Sephacryl S-200 gel filtration Bjerkandera adusta
1.11.1.19 ammonium sulfate precipitation-dialysis, Sepharose column chromatography, and Sephacryl S-200 gel filtration Bjerkandera adusta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.19 9.12
-
 after 48fold purification, with 2,6-dimethoxyphenol as substrate, at pH 3.0 and 70°C Bjerkandera adusta
1.11.1.19 18.6
-
 after 60fold purification, with 2,6-dimethoxyphenol as substrate, at pH 4.0 and 50°C Bjerkandera adusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 65% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 65% activity compared to 2,4-dichlorophenol Bjerkandera adusta CX-9 ?
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 65% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.19 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ 82% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
1.11.1.19 2,4,5-trichlorophenol + H2O2 + H+ 31% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 2,4,5-trichlorophenol + H2O2 + H+ 60% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,4,5-trichlorophenol +
-
 ?
1.11.1.19 2,4,5-trichlorophenol + H2O2 + H+ 31% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,4,5-trichlorophenol + H2O
-
 ?
1.11.1.19 2,4,5-trichlorophenol + H2O2 + H+ 64% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,4,5-trichlorophenol + H2O
-
 ?
1.11.1.19 2,4-dichlorophenol + H2O2 + H+ 100% activity Bjerkandera adusta ?
-
 ?
1.11.1.19 2,4-dichlorophenol + H2O2 + H+ 100% activity with 2,4-dichlorophenol, 88% activity with H2O2 Bjerkandera adusta oxidized 2,4-dichlorophenol + H2O
-
 ?
1.11.1.19 2,4-dichlorophenol + H2O2 + H+ 70% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,4-dichlorophenol + H2O
-
 ?
1.11.1.19 2,6-dichlorophenol + H2O2 + H+ 69% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 2,6-dichlorophenol + H2O2 + H+ 69% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,6-dichlorophenol + H2O
-
 ?
1.11.1.19 2,6-dichlorophenol + H2O2 + H+ 66% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2,6-dichlorophenol + H2O
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+ 75% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+ 75% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2,6-dimethoxyphenol + H2O
-
 ?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+ 100% activity with 2,6-dimethoxyphenol, 95% activity with H2O2 Bjerkandera adusta oxidized 2,6-dimethoxyphenol + H2O
-
 ?
1.11.1.19 2-dianisidine + H2O2 + H+ 85% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized 2-dianisidine + H2O
-
 ?
1.11.1.19 2-dianisidine + H2O2 + H+ 91% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 2-dianisidine + H2O
-
 ?
1.11.1.19 4-chlorophenol + H2O2 + H+ 25% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized 4-chlorophenol + H2O
-
 ?
1.11.1.19 Acid Blue 158 + H2O2 + H+
-
 Bjerkandera adusta oxidized Acid Blue 158 + H2O
-
 ?
1.11.1.19 Acid Blue 158 + H2O2 + H+ highest decolorization activity (about 90% after 12 h) Bjerkandera adusta oxidized Acid Blue 158 + H2O
-
 ?
1.11.1.19 Cibacet Brilliant Blue BG + H2O2 + H+
-
 Bjerkandera adusta oxidized Cibacet Brilliant Blue BG + H2O
-
 ?
1.11.1.19 commercial humic acid + H2O2 + H+ 81% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 coniferyl alcohol + H2O2 + H+ 97% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 coniferyl alcohol + H2O2 + H+ 97% activity compared to 2,4-dichlorophenol Bjerkandera adusta coniferaldehyde + H2O
-
 ?
1.11.1.19 coniferyl alcohol + H2O2 + H+ 65% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta coniferaldehyde + H2O
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ 91% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ 55% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized guaiacol + H2O
-
 ?
1.11.1.19 guaiacol + H2O2 + H+ 91% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized guaicol + H2O
-
 ?
1.11.1.19 humic acid + H2O2 + H+ 81% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized humic acid + H2O
-
 ?
1.11.1.19 humic acid + H2O2 + H+ 75% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized humic acid + H2O
-
 ?
1.11.1.19 Indigo Carmine + H2O2 + H+
-
 Bjerkandera adusta oxidized Indigo Carmine + H2O
-
 ?
1.11.1.19 L-DOPA + H2O2 + H+ 26% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 L-DOPA + H2O2 + H+ 26% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized L-DOPA + H2O
-
 ?
1.11.1.19 L-DOPA + H2O2 + H+ 70% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized L-DOPA + H2O
-
 ?
1.11.1.19 Methyl Green + H2O2 + H+
-
 Bjerkandera adusta oxidized Methyl Green + H2O
-
 ?
1.11.1.19 additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta ?
-
-
1.11.1.19 additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta CX-9 ?
-
-
1.11.1.19 additional information no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol Bjerkandera adusta CX-9 ?
-
 ?
1.11.1.19 o-dianisidine + H2O2 + H+ 85% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 pentachlorophenol + H2O2 + H+ 10% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized pentachlorophenol + H2O
-
 ?
1.11.1.19 Poly R-478 + H2O2 + H+
-
 Bjerkandera adusta oxidized Poly R-478 + H2O
-
 ?
1.11.1.19 pyrogallol + H2O2 + H+ 80% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?
1.11.1.19 pyrogallol + H2O2 + H+ 80% activity compared to 2,4-dichlorophenol Bjerkandera adusta oxidized pyrogallol + H2O
-
 ?
1.11.1.19 pyrogallol + H2O2 + H+ 52% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized pyrogallol + H2O
-
 ?
1.11.1.19 Reactive Blue 5 + H2O2
-
 Bjerkandera adusta phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
 ?
1.11.1.19 Reactive Dye Remazol Brilliant Violet 5R + H2O2 + H+
-
 Bjerkandera adusta oxidized Reactive Dye Remazol Brilliant Violet 5R + H2O
-
 ?
1.11.1.19 Remazol Brilliant Blue Reactif + H2O2 + H+
-
 Bjerkandera adusta oxidized Remazol Brilliant Blue Reactif + H2O
-
 ?
1.11.1.19 Remazol Brilliant Blue Reactif + H2O2 + H+ highest decolorization activity (about 90% after 12 h) Bjerkandera adusta oxidized Remazol Brilliant Blue Reactif + H2O
-
 ?
1.11.1.19 signayl alcohol + H2O2 + H+ 68% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta oxidized signaldehyde + H2O
-
 ?
1.11.1.19 signayl alcohol + H2O2 + H+ 94% activity compared to 2,4-dichlorophenol Bjerkandera adusta signaldehyde + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+ 99% activity compared to 2,4-dichlorophenol Bjerkandera adusta veratraldehyde + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+ 72% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta veratraldehyde + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+ 99% activity compared to 2,4-dichlorophenol Bjerkandera adusta CX-9 veratraldehyde + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+ 72% activity compared to 2,6-dimethoxyphenol Bjerkandera adusta CX-9 veratraldehyde + H2O
-
 ?
1.11.1.19 veratryl alcohol + H2O2 + H+ 99% activity compared to 2,4-dichlorophenol Bjerkandera adusta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 monomer 1 * 45000, SDS-PAGE Bjerkandera adusta
1.11.1.19 monomer 1 * 30000, SDS-PAGE Bjerkandera adusta
1.11.1.19 monomer 1 * 45221, calculated from amino acid sequence Bjerkandera adusta
1.11.1.19 monomer 1 * 30125, calculated from amino acid sequence Bjerkandera adusta

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 DyP
-
 Bjerkandera adusta
1.11.1.19 LiP BA45
-
 Bjerkandera adusta
1.11.1.19 MnP BA30
-
 Bjerkandera adusta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.19 50
-
-
 Bjerkandera adusta
1.11.1.19 70
-
-
 Bjerkandera adusta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.19 40 60 the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively Bjerkandera adusta
1.11.1.19 60
-
 the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60 °C, respectively. The half-life time of the enzyme at 40, 50, and 60°C increases to 7, 5, and 3 h in the presence 5 mM of Mg2+, respectively Bjerkandera adusta
1.11.1.19 60 80 the half-life times of the enzyme in the absence of 5 mM of Mn2+ are 8, 5, and 2 h at 60, 70, and 80°C, respectively. The half-life time of the purified peroxidase at 60, 70, and 80°C increases to 10, 7, and 4 h in the presence 5 mM of MnSO4, respectively Bjerkandera adusta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.101
-
 2,4-Dichlorophenol at pH 4.0 and 30°C Bjerkandera adusta
1.11.1.19 0.101
-
 2,4-Dichlorophenol at pH 3.0 and 70°C Bjerkandera adusta
1.11.1.19 0.207
-
 2,6-dimethoxyphenol at pH 4.0 and 50°C Bjerkandera adusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 3
-
-
 Bjerkandera adusta
1.11.1.19 4
-
-
 Bjerkandera adusta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.19 2 4 the enzyme maintains 50% of its original activity at pH 2.0 and 4.0 after incubation for 9 and 5 h at 50°C, respectively Bjerkandera adusta
1.11.1.19 3 5 the enzyme maintains 50% of its original activity at pH 3.0 and 5.0 after incubation for 10 and 6 h at 40°C, respectively Bjerkandera adusta

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme
-
 Bjerkandera adusta

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.19 5
-
 at pH 4.0 and 50°C Bjerkandera adusta Ag+

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 1.02
-
 2,4-Dichlorophenol at pH 3.0 and 70°C Bjerkandera adusta
1.11.1.19 1.0235
-
 2,4-Dichlorophenol at pH 4.0 and 30°C Bjerkandera adusta
1.11.1.19 1.37
-
 2,6-dimethoxyphenol at pH 4.0 and 50°C Bjerkandera adusta