BRENDA - Enzyme Database show

Purification and characterization of two novel peroxidases from the dye-decolorizing fungus Bjerkandera adusta strain CX-9

Bouacem, K.; Rekik, H.; Jaouadi, N.Z.; Zenati, B.; Kourdali, S.; El Hattab, M.; Badis, A.; Annane, R.; Bejar, S.; Hacene, H.; Bouanane-Darenfed, A.; Jaouadi, B.; Int. J. Biol. Macromol. 106, 636-646 (2017)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.19
2-mercaptoethanol
55% residual activity at 1 mM
Bjerkandera adusta
1.11.1.19
dithiothreitol
81% residual activity at 1 mM
Bjerkandera adusta
1.11.1.19
hydroxylamine
25% residual activity at 2 mM
Bjerkandera adusta
1.11.1.19
L-cystine
25% residual activity at 2 mM
Bjerkandera adusta
1.11.1.19
potassium cyanide
3% residual activity at 10 mM
Bjerkandera adusta
1.11.1.19
SDS
27% inhibition at 20 mM
Bjerkandera adusta
1.11.1.19
Sodium azide
5% residual activity at 10 mM
Bjerkandera adusta
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.19
0.099
-
2,4-Dichlorophenol
at pH 4.0 and 30°C
Bjerkandera adusta
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.19
Ca2+
110% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Cu2+
133% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Fe2+
109% activity at 5 mM
Bjerkandera adusta
1.11.1.19
K+
125% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Mg2+
180% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Mn2+
112% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Zn2+
104% activity at 5 mM
Bjerkandera adusta
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.19
Reactive Blue 5 + H2O2
Bjerkandera adusta
-
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.19
Bjerkandera adusta
-
-
-
1.11.1.19
Bjerkandera adusta CX-9
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.19
ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange column chromatography, and Sephacryl S-200 gel filtration
Bjerkandera adusta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.19
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
65% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
65% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
2,4,5-trichlorophenol + H2O2 + H+
31% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,4-dichlorophenol + H2O2 + H+
100% activity
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,6-dichlorophenol + H2O2 + H+
69% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,6-dimethoxyphenol + H2O2 + H+
75% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,6-dimethoxyphenol + H2O2 + H+
75% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
coinferyl alcohol + H2O2 + H+
97% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
commercial humic acid + H2O2 + H+
81% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
guaiacol + H2O2 + H+
91% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
guaiacol + H2O2 + H+
91% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
L-DOPA + H2O2 + H+
26% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
additional information
no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol
742674
Bjerkandera adusta
?
-
-
-
-
1.11.1.19
additional information
no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
-
1.11.1.19
o-dianisidine + H2O2 + H+
85% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
pyrogallol + H2O2 + H+
80% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
pyrogallol + H2O2 + H+
80% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
Reactive Blue 5 + H2O2
-
742674
Bjerkandera adusta
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
-
-
?
1.11.1.19
veratryl alcohol + H2O2 + H+
99% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.11.1.19
monomer
1 * 45000, SDS-PAGE; 1 * 45221, calculated from amino acid sequence
Bjerkandera adusta
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.19
50
-
-
Bjerkandera adusta
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.19
40
60
the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively
Bjerkandera adusta
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.19
0.101
-
2,4-Dichlorophenol
at pH 4.0 and 30°C
Bjerkandera adusta
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.19
4
-
-
Bjerkandera adusta
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.11.1.19
3
5
the enzyme maintains 50% of its original activity at pH 3.0 and 5.0 after incubation for 10 and 6 h at 40°C, respectively
Bjerkandera adusta
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.19
heme
-
Bjerkandera adusta
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.19
heme
-
Bjerkandera adusta
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.19
2-mercaptoethanol
55% residual activity at 1 mM
Bjerkandera adusta
1.11.1.19
dithiothreitol
81% residual activity at 1 mM
Bjerkandera adusta
1.11.1.19
hydroxylamine
25% residual activity at 2 mM
Bjerkandera adusta
1.11.1.19
L-cystine
25% residual activity at 2 mM
Bjerkandera adusta
1.11.1.19
potassium cyanide
3% residual activity at 10 mM
Bjerkandera adusta
1.11.1.19
SDS
27% inhibition at 20 mM
Bjerkandera adusta
1.11.1.19
Sodium azide
5% residual activity at 10 mM
Bjerkandera adusta
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.19
0.099
-
2,4-Dichlorophenol
at pH 4.0 and 30°C
Bjerkandera adusta
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.19
Ca2+
110% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Cu2+
133% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Fe2+
109% activity at 5 mM
Bjerkandera adusta
1.11.1.19
K+
125% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Mg2+
180% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Mn2+
112% activity at 5 mM
Bjerkandera adusta
1.11.1.19
Zn2+
104% activity at 5 mM
Bjerkandera adusta
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.19
Reactive Blue 5 + H2O2
Bjerkandera adusta
-
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.19
ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange column chromatography, and Sephacryl S-200 gel filtration
Bjerkandera adusta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.19
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
65% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
65% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
2,4,5-trichlorophenol + H2O2 + H+
31% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,4-dichlorophenol + H2O2 + H+
100% activity
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,6-dichlorophenol + H2O2 + H+
69% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,6-dimethoxyphenol + H2O2 + H+
75% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
2,6-dimethoxyphenol + H2O2 + H+
75% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
coinferyl alcohol + H2O2 + H+
97% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
commercial humic acid + H2O2 + H+
81% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
guaiacol + H2O2 + H+
91% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
guaiacol + H2O2 + H+
91% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
L-DOPA + H2O2 + H+
26% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
additional information
no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol
742674
Bjerkandera adusta
?
-
-
-
-
1.11.1.19
additional information
no activity with 2,4,6-trichlorophenol, 4-chlorophenol, and pentachlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
-
1.11.1.19
o-dianisidine + H2O2 + H+
85% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
pyrogallol + H2O2 + H+
80% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
1.11.1.19
pyrogallol + H2O2 + H+
80% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta CX-9
?
-
-
-
?
1.11.1.19
Reactive Blue 5 + H2O2
-
742674
Bjerkandera adusta
phthalate + 2,2'-disulfonyl azobenzene + 3-((4-amino-6-chloro-1,3,5-triazin-2-yl)amino)benzenesulfonate + H2O
-
-
-
?
1.11.1.19
veratryl alcohol + H2O2 + H+
99% activity compared to 2,4-dichlorophenol
742674
Bjerkandera adusta
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.1.19
monomer
1 * 45000, SDS-PAGE; 1 * 45221, calculated from amino acid sequence
Bjerkandera adusta
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.19
50
-
-
Bjerkandera adusta
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.19
40
60
the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively
Bjerkandera adusta
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.19
0.101
-
2,4-Dichlorophenol
at pH 4.0 and 30°C
Bjerkandera adusta
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.19
4
-
-
Bjerkandera adusta
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.11.1.19
3
5
the enzyme maintains 50% of its original activity at pH 3.0 and 5.0 after incubation for 10 and 6 h at 40°C, respectively
Bjerkandera adusta
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.11.1.19
1.0235
-
2,4-Dichlorophenol
at pH 4.0 and 30°C
Bjerkandera adusta
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.11.1.19
1.0235
-
2,4-Dichlorophenol
at pH 4.0 and 30°C
Bjerkandera adusta