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Literature summary extracted from

  • Lu, L.; Yang, Y.; Lin, H.; Gao, F.; Zhao, Y.
    Construction, expression and characterization of fusion enzyme containing azoreductase and glucose 1-dehydrogenase for dye removal (2014), Int. Biodeter. Biodegrad., 87, 81-86 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.6 dodecyl trimethyl ammonium bromide
-
 Shewanella oneidensis
1.7.1.6 sodium dodecyl benzene sulfonate
-
 Shewanella oneidensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 functional recombinant expression of the chimeric gene GFA, in which both GDH stop codon and the sequence encoding AZR signal peptide are removed, in Escherichia coli strain BL21 from plasmid pET28a(+) Shewanella oneidensis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.6 additional information construction of a chimeric artificial bifunctional enzyme chimeric enzyme composed of the two oligomeric enzymes, azoreductase (AZR) from Shewanella oneidensis MR-1 and the alkaline-resistant NAD(P)-dependent glucose 1-dehydrogenase (LsGDH) from Lysinibacillus sphaericus G10. The reaction is extended to a recycling system. The fusion enzyme exhibits a higher stability and suitability for high-temperature application than the parental wild-type enzyme Shewanella oneidensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 Cd2+ complete inhibition at 25 mM Shewanella oneidensis
1.7.1.6 cetyl dimethylethyl ammonium bromide
-
 Shewanella oneidensis
1.7.1.6 Cetylpyridinium chloride
-
 Shewanella oneidensis
1.7.1.6 Mn2+ inhibits at 25 mM Shewanella oneidensis
1.7.1.6 SDS
-
 Shewanella oneidensis
1.7.1.6 tetradecylammonium bromide
-
 Shewanella oneidensis
1.7.1.6 Zn2+ inhibits at 25 mM Shewanella oneidensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.00476
-
 Methyl red recombinant chimeric fusion protein AZR-GDH, pH 7.0, 40°C Shewanella oneidensis
1.7.1.6 0.00916
-
 Methyl red recombinant wild-type AZR, pH 7.0, 40°C Shewanella oneidensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.6 Ca2+ activates 1.84fold at 25 mM Shewanella oneidensis
1.7.1.6 Mg2+ activates 1.28fold at 25 mM Shewanella oneidensis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Shewanella oneidensis Q8EBE0
-
-
1.7.1.6 Shewanella oneidensis MR-1 / ATCC 700550 Q8EBE0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6 recombinant chimeric fusion protein AZR-GDH from Escherichia coli strain BL21 Shewanella oneidensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 153.16
-
 purified wild-type AZR, 40°C, pH 7.0, substrate methyl red Shewanella oneidensis
1.7.1.6 160.62
-
 purified recombinant fusion protein AZR-GDH, 40°C, pH 7.0, substrate methyl red Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 methyl red + 2 NADH + 2 H+ i.e. 4-dimethylaminoazobenzene-2'-carboxylic acid Shewanella oneidensis anthranilate + 4-amino-N,N-dimethylaniline + 2 NAD+
-
 ?
1.7.1.6 methyl red + 2 NADH + 2 H+ i.e. 4-dimethylaminoazobenzene-2'-carboxylic acid Shewanella oneidensis MR-1 / ATCC 700550 anthranilate + 4-amino-N,N-dimethylaniline + 2 NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 ? x * 55000, recombinant chimeric fusion protein AZR-GDH, SDS-PAGE Shewanella oneidensis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 azoreductase
-
 Shewanella oneidensis
1.7.1.6 AZR
-
 Shewanella oneidensis
1.7.1.6 NADPH-dependent azoreductase Azr UniProt Shewanella oneidensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 40
-
 recombinant chimeric fusion protein AZR-GDH Shewanella oneidensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.1.6 20 60 recombinant chimeric fusion protein AZR-GDH, activity range, 63.2% and 4.0% of maximal activity at 55°C and 60°C, respectively. The residual activity of the parental wild-type azoreductase decreases to 1% at 55°C Shewanella oneidensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.6 82.17
-
 Methyl red recombinant wild-type AZR, pH 7.0, 40°C Shewanella oneidensis
1.7.1.6 102.92
-
 Methyl red recombinant chimeric fusion protein AZR-GDH, pH 7.0, 40°C Shewanella oneidensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 7
-
 recombinant chimeric fusion protein AZR-GDH Shewanella oneidensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.6 4 9 recombinant chimeric fusion protein AZR-GDH, activity range, profile overview Shewanella oneidensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.6 4 8 completely stable at pH 7.0-7.5, 50% activity remaining at pH 5.0 and pH 7.8, 20% activity remaining at pH 4.0 and pH 8.0 Shewanella oneidensis
1.7.1.6 7 7.5 purified recombinant chimeric fusion protein AZR-GDH, completely stable at Shewanella oneidensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 NADH
-
 Shewanella oneidensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.6 8970.5
-
 Methyl red recombinant wild-type AZR, pH 7.0, 40°C Shewanella oneidensis
1.7.1.6 21621.8
-
 Methyl red recombinant chimeric fusion protein AZR-GDH, pH 7.0, 40°C Shewanella oneidensis