EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.17 | overexpression in Escherichia coli BL21(DE3) cells harboring plasmid pME4141 | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.17 | Fe2+ | the thermodynamic properties of Fe2+ binding to the 2-His-1-carboxylate facial triad in alpha-ketoglutarate/taurine dioxygenase (TauD) are explored using isothermal titration calorimetry. Direct titrations of Fe2+ into TauD and chelation experiments involving the titration of thylenediaminetetraacetic acid into Fe2+-TauD are performed under an anaerobic environment to yield a binding equilibrium of 2400000 (Kd = 43 nM) and a DELTAG(0) value of -10.1 kcal/mol. Further analysis of the enthalpy/entropy contributions indicates a highly enthalpic binding event, where DELTAH = -11.6 kcal/mol. Investigations into the unfavorable entropy term leads to the observation of water molecules becoming organized within the Fe2+-TauD structure | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.17 | Escherichia coli | P37610 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.17 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.17 | taurine + 2-oxoglutarate + O2 | - |
Escherichia coli | sulfite + aminoacetaldehyde + succinate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.17 | alpha-ketoglutarate/taurine dioxygenase | - |
Escherichia coli |
1.14.11.17 | TauD | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.11.17 | 37 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.17 | 8 | - |
assay at | Escherichia coli |