Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chaurasia, P.K.; Bharati, S.L.; Yadava, S.; Yadav, R.S.
    Purification, characterization and synthetic application of a thermally stable laccase from Hexagonia tenuis MTCC-1119 (2015), Indian J. Biochem. Biophys., 52, 60-67 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 synthesis selective biotransformation of aromatic methyl group to aldehyde group in presence of diammonium salt of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as the mediator Hexagonia tenuis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.036
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Hexagonia tenuis
1.10.3.2 0.08
-
 2,6-dimethoxyphenol pH 4.5, 37°C Hexagonia tenuis
1.10.3.2 0.087
-
 syringaldazine pH 6.0, 50°C Hexagonia tenuis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Hexagonia tenuis
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Hexagonia tenuis
-
-
-
1.10.3.2 Hexagonia tenuis MTCC-1119
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Hexagonia tenuis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture filtrate
-
 Hexagonia tenuis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Hexagonia tenuis ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Hexagonia tenuis MTCC-1119 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Hexagonia tenuis ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Hexagonia tenuis MTCC-1119 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Hexagonia tenuis ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Hexagonia tenuis MTCC-1119 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 100000, SDS-PAGE Hexagonia tenuis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
-
 Hexagonia tenuis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 2.54
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Hexagonia tenuis
1.10.3.2 2.54
-
 2,6-dimethoxyphenol pH 4.5, 37°C Hexagonia tenuis
1.10.3.2 2.54
-
 syringaldazine pH 6.0, 50°C Hexagonia tenuis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.5
-
 substrate: 2,6-dimethoxyphenol Hexagonia tenuis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 4
-
 1 h, most stable at pH 4.0 Hexagonia tenuis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 29.2
-
 syringaldazine pH 6.0, 50°C Hexagonia tenuis
1.10.3.2 31.7
-
 2,6-dimethoxyphenol pH 4.5, 37°C Hexagonia tenuis
1.10.3.2 70.5
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C Hexagonia tenuis