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Literature summary extracted from

  • Wang, H.; Liu, B.; Li, H.; Zhang, S.
    Identification and biochemical characterization of polyamine oxidases in amphioxus Implications for emergence of vertebrate-specific spermine and acetylpolyamine oxidases (2016), Gene, 575, 429-437 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.13 wild-type and mutant enzymes expressed in Escherichia coli Branchiostoma japonicum
1.5.3.17 wild-type and mutant enzymes expressed in Escherichia coli Branchiostoma japonicum

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.13 H69E compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
1.5.3.13 H69Q compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
1.5.3.13 K315M compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
1.5.3.13 T467S compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
1.5.3.13 T467Y compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value Branchiostoma japonicum
1.5.3.17 H64E Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
1.5.3.17 H64Q Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
1.5.3.17 H69E compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
1.5.3.17 H69Q compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
1.5.3.17 K301M Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
1.5.3.17 K315M compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
1.5.3.17 T460S Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
1.5.3.17 T460Y Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
1.5.3.17 T467S compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
1.5.3.17 T467Y compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.13 N1,N4-bis(2,3-butadienyl)-1,4-butanediamine 2.5 mM, 70% inhibition of N1-acetylspermine oxidation Branchiostoma japonicum
1.5.3.17 N1,N4-bis(2,3-butadienyl)-1,4-butanediamine 2.5 mM, 50% inhibition of spermidine oxidation, 70% inhibition of N1-acetylspermine oxidation; 2.5 mM, 50% inhibition of spermidine oxidation, 70% inhibition of spermine oxidation Branchiostoma japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.13 0.0369
-
 N1-acetylspermine 37°C, pH 8.0, wild-type enzyme Branchiostoma japonicum
1.5.3.13 0.043
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme K315M Branchiostoma japonicum
1.5.3.13 0.046
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467Y Branchiostoma japonicum
1.5.3.13 0.058
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467S Branchiostoma japonicum
1.5.3.13 0.062
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69E Branchiostoma japonicum
1.5.3.13 0.071
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69Q Branchiostoma japonicum
1.5.3.17 0.007
-
 spermidine 37°C, pH 9.5, wild-type enzyme Branchiostoma japonicum
1.5.3.17 0.00836
-
 spermine 37°C, pH 8.5, wild-type enzyme Branchiostoma japonicum
1.5.3.17 0.012
-
 spermine 37°C, pH 8.5, mutant enzyme T460S Branchiostoma japonicum
1.5.3.17 0.0124
-
 spermidine 37°C, pH 9.5, wild-type enzyme Branchiostoma japonicum
1.5.3.17 0.013
-
 spermine 37°C, pH 8.5, mutant enzyme T460Y Branchiostoma japonicum
1.5.3.17 0.016
-
 spermidine 37°C, pH 9.5, mutant enzyme K301M Branchiostoma japonicum
1.5.3.17 0.017
-
 spermine 37°C, pH 8.5, mutant enzyme K301M Branchiostoma japonicum
1.5.3.17 0.017
-
 spermidine 37°C, pH 9.5, mutant enzyme T460Y Branchiostoma japonicum
1.5.3.17 0.019
-
 spermidine 37°C, pH 9.5, mutant enzyme H64E Branchiostoma japonicum
1.5.3.17 0.019
-
 spermidine 37°C, pH 9.5, mutant enzyme T460S Branchiostoma japonicum
1.5.3.17 0.02
-
 spermidine 37°C, pH 9.5, mutant enzyme T467Y Branchiostoma japonicum
1.5.3.17 0.021
-
 spermidine 37°C, pH 9.5, mutant enzyme H64Q Branchiostoma japonicum
1.5.3.17 0.0229
-
 spermidine 37°C, pH 9.5, mutant enzyme H69Q Branchiostoma japonicum
1.5.3.17 0.024
-
 spermine 37°C, pH 8.5, mutant enzyme H64E Branchiostoma japonicum
1.5.3.17 0.025
-
 spermidine 37°C, pH 9.5, mutant enzyme H69E Branchiostoma japonicum
1.5.3.17 0.0269
-
 spermine 37°C, pH 8.5, mutant enzyme H64Q Branchiostoma japonicum
1.5.3.17 0.031
-
 spermidine 37°C, pH 9.5, mutant enzyme K315M Branchiostoma japonicum
1.5.3.17 0.0369
-
 N1-acetylspermine 37°C, pH 8.0, wild-type enzyme Branchiostoma japonicum
1.5.3.17 0.043
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme K315M Branchiostoma japonicum
1.5.3.17 0.046
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467Y Branchiostoma japonicum
1.5.3.17 0.058
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467S Branchiostoma japonicum
1.5.3.17 0.062
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69E Branchiostoma japonicum
1.5.3.17 0.071
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69Q Branchiostoma japonicum
1.5.3.17 0.182
-
 spermidine 37°C, pH 9.5, mutant enzyme T467S Branchiostoma japonicum

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.13 Branchiostoma japonicum A0A0N7IZL9
-
-
1.5.3.17 Branchiostoma japonicum
-
-
-
1.5.3.17 Branchiostoma japonicum A0A0N7IZL9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.13
-
 Branchiostoma japonicum
1.5.3.17
-
 Branchiostoma japonicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.13 gill
-
 Branchiostoma japonicum
-
1.5.3.13 hepatic cecum
-
 Branchiostoma japonicum
-
1.5.3.13 hindgut
-
 Branchiostoma japonicum
-
1.5.3.13 muscle
-
 Branchiostoma japonicum
-
1.5.3.13 notochord
-
 Branchiostoma japonicum
-
1.5.3.13 ovary
-
 Branchiostoma japonicum
-
1.5.3.13 testis
-
 Branchiostoma japonicum
-
1.5.3.17 gill
-
 Branchiostoma japonicum
-
1.5.3.17 hepatic cecum
-
 Branchiostoma japonicum
-
1.5.3.17 hindgut
-
 Branchiostoma japonicum
-
1.5.3.17 muscle
-
 Branchiostoma japonicum
-
1.5.3.17 notochord
-
 Branchiostoma japonicum
-
1.5.3.17 notochord Bjpao1 is predominantly expressed in the notochord, testis and ovary Branchiostoma japonicum
-
1.5.3.17 ovary
-
 Branchiostoma japonicum
-
1.5.3.17 ovary Bjpao1 is predominantly expressed in the notochord, testis and ovary Branchiostoma japonicum
-
1.5.3.17 testis
-
 Branchiostoma japonicum
-
1.5.3.17 testis Bjpao1 is predominantly expressed in the notochord, testis and ovary Branchiostoma japonicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.13 additional information Bjpao2 shows no activity with spermine Branchiostoma japonicum ?
-
 ?
1.5.3.13 N1-acetylspermine + O2 + H2O
-
 Branchiostoma japonicum spermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.17 additional information Bjpao1 shows no activity with N1-acetylspermine Branchiostoma japonicum ?
-
 ?
1.5.3.17 additional information Bjpao2 shows no activity with spermine Branchiostoma japonicum ?
-
 ?
1.5.3.17 N1-acetylspermine + O2 + H2O
-
 Branchiostoma japonicum spermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.17 spermidine + O2 + H2O
-
 Branchiostoma japonicum putrescine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 spermine + O2 + H2O
-
 Branchiostoma japonicum spermidine + 3-aminopropanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.13 ? x * 59000, SDS-PAGE Branchiostoma japonicum
1.5.3.17 ? x * 56000, SDS-PAGE Branchiostoma japonicum
1.5.3.17 ? x * 59000, SDS-PAGE Branchiostoma japonicum

Synonyms

EC Number Synonyms Comment Organism
1.5.3.13 Bjpao2
-
 Branchiostoma japonicum
1.5.3.17 Bjpao1
-
 Branchiostoma japonicum
1.5.3.17 Bjpao2
-
 Branchiostoma japonicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.13 30 37
-
 Branchiostoma japonicum
1.5.3.17 30 37
-
 Branchiostoma japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.13 8
-
 substrate: N1-acetylspermine Branchiostoma japonicum
1.5.3.17 8
-
 substrate: N1-acetylspermine Branchiostoma japonicum
1.5.3.17 8.5
-
 substrate: spermine Branchiostoma japonicum
1.5.3.17 9
-
 substrate: spermidine Branchiostoma japonicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.3.13 Branchiostoma japonicum calculated from sequence
-
 5.13
1.5.3.17 Branchiostoma japonicum calculated from sequence
-
 4.9
1.5.3.17 Branchiostoma japonicum calculated from sequence
-
 5.13