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Literature summary extracted from

  • El Hadrami, A.; Islam, M.R.; Adam, L.R.; Daayf, F.
    A cupin domain-containing protein with a quercetinase activity (VdQase) regulates Verticillium dahliaes pathogenicity and contributes to counteracting host defenses (2015), Front. Plant Sci., 6, 440 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.24 gene VDAG_02532, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis Verticillium dahliae

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.24 additional information generation of VdQase knock-out mutants through Agrobacterium tumefasciens-mediated transformation, phenotypes, detailed overview Verticillium dahliae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.24 Cu-EDTA abolishes the quercetinase activity Verticillium dahliae
1.13.11.24 kojic acid abolishes the quercetinase activity Verticillium dahliae
1.13.11.24 prohexadione-Ca abolishes the quercetinase activity Verticillium dahliae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.24 nucleus
-
 Verticillium dahliae 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.24 Cu2+ activates Verticillium dahliae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.24 quercetin + O2 Verticillium dahliae
-
 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+
-
 ?
1.13.11.24 quercetin + O2 Verticillium dahliae ATCC MYA-4575
-
 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.24 Verticillium dahliae G2WY50 isolates namely Vd1396-9 (Vd9) and Vd1398-21 (Vd21)
-
1.13.11.24 Verticillium dahliae ATCC MYA-4575 G2WY50 isolates namely Vd1396-9 (Vd9) and Vd1398-21 (Vd21)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.24 separation of isozymes Verticillium dahliae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.24 additional information enzyme expression in cells grown on medium with rutin, quercetin, and naringenin is high, although low on phloroglucinol Verticillium dahliae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.24 quercetin + O2
-
 Verticillium dahliae 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+
-
 ?
1.13.11.24 quercetin + O2
-
 Verticillium dahliae ATCC MYA-4575 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.24 cupin domain-containing protein
-
 Verticillium dahliae
1.13.11.24 quercetinase
-
 Verticillium dahliae
1.13.11.24 VdQase
-
 Verticillium dahliae

Expression

EC Number Organism Comment Expression
1.13.11.24 Verticillium dahliae cell growth on rutin, quercetin, and naringenin induces the enzyme expression, while growth on phloroglucinol does not up

General Information

EC Number General Information Comment Organism
1.13.11.24 malfunction the pathogenicity of VdQase knock-out mutants generated through Agrobacterium tumefasciens-mediated transformation is significantly reduced on susceptible potato cultivar Kennebec compared to wild-type isolates. Phenotype with a higher accumulation of flavonols in the stems of infected potatoes and a higher concentration of rutin in the leaves in response to the VdQase mutants as compared to wild-type isolates Verticillium dahliae
1.13.11.24 physiological function the enzyme is a cupin domain-containing protein with dioxygenase activity and quercetinase activity (VdQase) regulating Verticillium dahliaes pathogenicity in potato roots and contributing to counteraction against host defenses. Involvement of enzyme VdQase in the catabolism of quercetin and possibly other flavonols in planta. Involvement of VdQase in the interference with signaling, suggesting a role in pathogenicity. It is hypothesized that the by-product of dioxygenation 2-protocatechuoylphloroglucinolcarboxylic acid, after dissociating into phloroglucinol and protocatechuoyl moieties, becomes a starting point for benzoic acid and salicylic acid, thereby interfering with the jasmonate pathway and affecting the interaction outcome. These events may be key factors for Verticillium dahliaes in countering potato defenses and becoming notorious in the rhizosphere. Role of VdQase in rutin and quercetin utilization, overview Verticillium dahliae