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Literature summary extracted from

  • Shi, S.; Ma, F.; Sun, T.; Li, A.; Zhou, J.; Qu, Y.
    Multistep conversion of cresols by phenol hydroxylase and 2,3-dihydroxy-biphenyl 1,2-dioxygenase (2014), Front. Environ. Sci. Eng., 8, 539-546 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.13.11.39 synthesis a multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol Paraburkholderia xenovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 gene bphC, recombinant expression in Escherichia coli Paraburkholderia xenovorans
1.14.13.7 expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Paraburkholderia xenovorans
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2 Pseudomonas sp.
-
 catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2 Pseudomonas sp. OX1
-
 catechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Paraburkholderia xenovorans
-
-
-
1.14.13.7 Pseudomonas sp.
-
-
-
1.14.13.7 Pseudomonas sp. OX1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3-methylcatechol + O2
-
 Paraburkholderia xenovorans 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 4-methylcatechol + O2 low activity Paraburkholderia xenovorans 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Paraburkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 additional information multistep conversion system composed of Pseudomonas sp. phenol hydroxylase (PHIND) and Burkholderia xenovorans 2,3-dihydroxy-biphenyl 1,2-dioxygenase (BphCLA-4) from is used to synthesize methylcatechols and semialdehydes from o- and m-cresol, substrate specificity and molecular docking, product identification by HPLC-MS, overview Paraburkholderia xenovorans ?
-
 ?
1.14.13.7 3-cresol + NADPH + H+ + O2
-
 Pseudomonas sp. 3-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 3-cresol + NADPH + H+ + O2
-
 Pseudomonas sp. OX1 3-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 4-cresol + NADPH + H+ + O2
-
 Pseudomonas sp. 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 4-cresol + NADPH + H+ + O2
-
 Pseudomonas sp. OX1 4-methylcatechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2
-
 Pseudomonas sp. catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2
-
 Pseudomonas sp. OX1 catechol + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-dihydroxy-biphenyl 1,2-dioxygenase
-
 Paraburkholderia xenovorans
1.13.11.39 BphCLA-4
-
 Paraburkholderia xenovorans
1.14.13.7 phenol hydroxylase
-
 Pseudomonas sp.
1.14.13.7 PHIND
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 8
-
-
 Paraburkholderia xenovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.39 5 10 over 60% at pH 6.0 and 9.0, and less than 50% catalytic efficiency at pH 5.0 and 10.0, strain BphCLA-4 Paraburkholderia xenovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 NADPH
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.13.11.39 additional information enzyme structure homology modeling, overview Paraburkholderia xenovorans