BRENDA - Enzyme Database

Engineering of beta-carotene hydroxylase and ketolase for astaxanthin overproduction in Saccharomyces cerevisiae

Wang, R.; Gu, X.; Yao, M.; Pan, C.; Liu, H.; Xiao, W.; Wang, Y.; Yuan, Y.; Front. Chem. Sci. Engin. 11, 89-99 (2017)
No PubMed abstract available

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.3.5.B4
synthesis
construction of an astaxanthin biosynthesis pathway in Saccharomyces cerevisiae by introducing heterologous beta-carotene hydroxylase (CrtZ) and beta-carotene ketolase (CrtW) into an existing high beta-carotene producing strain. Astaxanthin yield of 3.1 mg/g dry cell weight are achieved. After change of promoter and hrough high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reaches to 81.0 mg/l
Brevundimonas vesicularis
Cloned(Commentary)
EC Number
Commentary
Organism
1.3.5.B4
expression in Saccharomyces cerevisiae
Brevundimonas vesicularis
1.14.11.B16
expressed in Saccharomyces cerevisiae strain Sc118030
Brevundimonas vesicularis
1.14.15.24
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight; expressed in Saccharomyces cerevisiae
Alcaligenes sp. PC-1
1.14.99.63
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight
Brevundimonas vesicularis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
Brevundimonas vesicularis
-
canthaxanthin + succinate + CO2 + 4 H+
-
-
?
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
Brevundimonas vesicularis DC263
-
canthaxanthin + succinate + CO2 + 4 H+
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Alcaligenes sp. PC-1
-
beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Alcaligenes sp. PC-1
overall reaction
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Alcaligenes sp. PC-1
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.B4
Brevundimonas vesicularis
Q0H2C9
-
-
1.3.5.B4
Brevundimonas vesicularis DC263
Q0H2C9
-
-
1.14.11.B16
Brevundimonas vesicularis
-
-
-
1.14.11.B16
Brevundimonas vesicularis DC263
-
-
-
1.14.15.24
Alcaligenes sp. PC-1
-
-
-
1.14.15.24
Alcaligenes sp. PC-1
Q44262
-
-
1.14.99.63
Brevundimonas vesicularis
Q0H2C9
-
-
1.14.99.63
Brevundimonas vesicularis DC263
Q0H2C9
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
-
742573
Brevundimonas vesicularis
canthaxanthin + succinate + CO2 + 4 H+
-
-
-
?
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
-
742573
Brevundimonas vesicularis DC263
canthaxanthin + succinate + CO2 + 4 H+
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
742573
Alcaligenes sp. PC-1
beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
overall reaction
742573
Alcaligenes sp. PC-1
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
742573
Alcaligenes sp. PC-1
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
1.3.5.B4
synthesis
construction of an astaxanthin biosynthesis pathway in Saccharomyces cerevisiae by introducing heterologous beta-carotene hydroxylase (CrtZ) and beta-carotene ketolase (CrtW) into an existing high beta-carotene producing strain. Astaxanthin yield of 3.1 mg/g dry cell weight are achieved. After change of promoter and hrough high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reaches to 81.0 mg/l
Brevundimonas vesicularis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.B4
expression in Saccharomyces cerevisiae
Brevundimonas vesicularis
1.14.11.B16
expressed in Saccharomyces cerevisiae strain Sc118030
Brevundimonas vesicularis
1.14.15.24
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight
Alcaligenes sp. PC-1
1.14.15.24
expressed in Saccharomyces cerevisiae
Alcaligenes sp. PC-1
1.14.99.63
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight
Brevundimonas vesicularis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
Brevundimonas vesicularis
-
canthaxanthin + succinate + CO2 + 4 H+
-
-
?
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
Brevundimonas vesicularis DC263
-
canthaxanthin + succinate + CO2 + 4 H+
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Alcaligenes sp. PC-1
-
beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Alcaligenes sp. PC-1
overall reaction
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.24
beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Alcaligenes sp. PC-1
-
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
-
742573
Brevundimonas vesicularis
canthaxanthin + succinate + CO2 + 4 H+
-
-
-
?
1.14.11.B16
beta-carotene + 2-oxoglutarate + O2 + H2O
-
742573
Brevundimonas vesicularis DC263
canthaxanthin + succinate + CO2 + 4 H+
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
742573
Alcaligenes sp. PC-1
beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
overall reaction
742573
Alcaligenes sp. PC-1
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
742573
Alcaligenes sp. PC-1
zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?