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Literature summary extracted from

  • da Silva, R.R.; de Oliveira, L.C.; Juliano, M.A.; Juliano, L.; de Oliveira, A.H.; Rosa, J.C.; Cabral, H.
    Biochemical and milk-clotting properties and mapping of catalytic subsites of an extracellular aspartic peptidase from basidiomycete fungus Phanerochaete chrysosporium (2017), Food Chem., 225, 45-54 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.23.49 food industry the peptidase shows maximal milk clotting activity at 60-65 °C and maintenance of enzymatic activity above 80% in the presence of 20 mM CaCl2 Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.49 Al3+ 1 mM, 47% residual activity Phanerodontia chrysosporium
3.4.23.49 Cu2+ 1 mM, 77% residual activity Phanerodontia chrysosporium
3.4.23.49 guanidine hydrochloride 50 mM, 40% residual activity Phanerodontia chrysosporium
3.4.23.49 pepstatin A 5 mM, complete loss of activity Phanerodontia chrysosporium
3.4.23.49 SDS 0.02%, complete loss of activity Phanerodontia chrysosporium
3.4.23.49 Tween 20 up to 1%, 80% residual activity Phanerodontia chrysosporium
3.4.23.49 Urea 50 mM, 72% residual activity Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.49 0.00012
-
 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 0.00024
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 0.0003
-
 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 0.00043
-
 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 0.00064
-
 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.49 extracellular
-
 Phanerodontia chrysosporium
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.49 Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.49
-
 Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.49 750
-
 pH 4.5, 40°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.49 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KLK + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.49 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KLR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.49 2-aminobenzoyl-KLRSSVQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KLR + SSVQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.49 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KMR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.49 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KNR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.49 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Phanerodontia chrysosporium 2-aminobenzoyl-KQR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
3.4.23.49 additional information enzyme displays restriction of catalysis at the S1 subsite, with a preference for lysine, arginine, leucine, tyrosine, and phenylalanine residues. No hydrolysis of the substrate peptide is observed with amino acids A, D, E, H, I, M, N, P, Q, S, T, V, or W in the S1 position. At S2 and S1' subsites, the enzyme exhibits a good acceptance of amino acids L, M, E, D, R and S, Y, Q, respectively Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.49 ? x * 38000, SDS-PAGE Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.49 50
-
-
 Phanerodontia chrysosporium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.49 65
-
 50% of maximum activity Phanerodontia chrysosporium
3.4.23.49 70
-
 40% of maximum activity Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.49 55
-
 stable for 1 h Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.49 0.64
-
 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 0.8
-
 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 0.824
-
 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 1.15
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 1.54
-
 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.49 4.5
-
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.49 5.5
-
 46% of maximum activity Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.49 3 8
-
 Phanerodontia chrysosporium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.23.49 1860
-
 2-aminobenzoyl-KNRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 2000
-
 2-aminobenzoyl-KQRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 2133
-
 2-aminobenzoyl-KMRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 2406
-
 2-aminobenzoyl-KLKSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 4732
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium
3.4.23.49 4792
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 4.5, 40°C Phanerodontia chrysosporium