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Literature summary extracted from

  • Silva, J.C.; Guimaraes, L.H.; Salgado, J.C.; Furriel, R.P.; Polizeli, M.L.; Rosa, J.C.; Jorge, J.A.
    Purification and biochemical characterization of glucose-cellobiose-tolerant cellulases from Scytalidium thermophilum (2013), Folia Microbiol. (Praha), 58, 561-568 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 2-mercaptoethanol 1 mM, 242% of initial activity Mycothermus thermophilus
3.2.1.4 cysteine 1 mM, 174% of initial activity Mycothermus thermophilus
3.2.1.4 dithiothreitol 1 mM, 189% of initial activity Mycothermus thermophilus
3.2.1.4 Mn2+ 1 mM, 177% of initial activity Mycothermus thermophilus
3.2.1.176 2-mercaptoethanol 1 mM, 177% of initial activity Mycothermus thermophilus
3.2.1.176 cysteine 1 mM, 132% of initial activity Mycothermus thermophilus
3.2.1.176 dithiothreitol 1 mM, 158% of initial activity Mycothermus thermophilus
3.2.1.176 Mn2+ 1 mM, 155% of initial activity Mycothermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ 1 mM, 72% of initial activity Mycothermus thermophilus
3.2.1.4 Hg2+ 1 mM, complete loss of activity Mycothermus thermophilus
3.2.1.4 NH4+ 1 mM, 84% of initial activity Mycothermus thermophilus
3.2.1.176 Cu2+ 1 mM, 94% of initial activity Mycothermus thermophilus
3.2.1.176 Hg2+ 1 mM complete loss of activity Mycothermus thermophilus
3.2.1.176 NH4+ 1 mM, 70% of initial activity Mycothermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 38000
-
 gel filtration Mycothermus thermophilus
3.2.1.176 50400
-
 isoform cellulase I, gel filtration Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Mycothermus thermophilus
-
-
-
3.2.1.176 Mycothermus thermophilus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein 93% carbohydrate content Mycothermus thermophilus
3.2.1.176 glycoprotein 11% carbohydrate content Mycothermus thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Mycothermus thermophilus
3.2.1.176
-
 Mycothermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 51.3
-
 pH 4.0, 55°C Mycothermus thermophilus
3.2.1.176 11.7
-
 pH 4.0, 55°C Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Mycothermus thermophilus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Mycothermus thermophilus ?
-
 ?
3.2.1.4 additional information no substrates: starch, xylan, cellobiose, filter paper Mycothermus thermophilus ?
-
 ?
3.2.1.176 avicel + H2O
-
 Mycothermus thermophilus ?
-
 ?
3.2.1.176 carboxymethyl cellulose + H2O
-
 Mycothermus thermophilus cellobiose
-
 ?
3.2.1.176 additional information no substrates: starch, xylan, cellobiose. Filter paper is a very poor substrate Mycothermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 45600, SDS-PAGE Mycothermus thermophilus
3.2.1.176 monomer 1 * 51200, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 65
-
-
 Mycothermus thermophilus
3.2.1.176 60 65
-
 Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 1 h, stable Mycothermus thermophilus
3.2.1.176 60
-
 1 h, stable Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5
-
-
 Mycothermus thermophilus
3.2.1.176 4
-
-
 Mycothermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Mycothermus thermophilus isoelectric focusing
-
 6.9
3.2.1.176 Mycothermus thermophilus isoelectric focusing
-
 6.2