Literature summary extracted from

Shuman, K.; Hanson, T.
A sulfide quinone oxidoreductase from Chlorobaculum tepidum displays unusual kinetic properties (2016), FEMS Microbiol. Lett., 363, 1-8 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.8.5.4	expressed in Escherichia coli BL21(DE3) pLysScells	Chlorobaculum tepidum

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8.5.4	1.95	-	Sulfide	recombinant enzyme, at pH 7.4 and 47°C	Chlorobaculum tepidum

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.8.5.4	43500	-	gel filtration	Chlorobaculum tepidum

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.5.4	Chlorobaculum tepidum	Q8KDG3	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.8.5.4	Ni-NTA column chromatography	Chlorobaculum tepidum

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8.5.4	12.7	-	purified recombinant enzyme, at pH 7.4 and 47°C	Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.5.4	sulfide + decylubiquinone	-	Chlorobaculum tepidum	sulfur + decylubiquinol	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.8.5.4	monomer	1 * 45800, calculated from amino acid sequence	Chlorobaculum tepidum

Synonyms

EC Number	Synonyms	Comment	Organism
1.8.5.4	CT1087	-	Chlorobaculum tepidum
1.8.5.4	SQR	-	Chlorobaculum tepidum

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.8.5.4	54	-	Sulfide	recombinant enzyme, at pH 7.4 and 47°C	Chlorobaculum tepidum

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.8.5.4	FAD	-	Chlorobaculum tepidum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)