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Literature summary extracted from

  • Ceveryshetty, J.; Suvekbala, V.; Varadamshetty, G.; Phale, P.S.
    Metabolism of 2-, 3- and 4-hydroxybenzoates by soil isolates Alcaligenes sp. strain PPH and Pseudomonas sp. strain PPD (2007), FEMS Microbiol. Lett., 268, 59-66 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + H+ + O2 Alcaligenes sp. PPH
-
 protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + H+ + O2 Pseudomonas sp. PPD
-
 protocatechuate + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Alcaligenes sp. PPH
-
-
-
1.14.13.2 Pseudomonas sp. PPD
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.2 0.016
-
 at pH 7.5, temperature not specified in the publication Alcaligenes sp. PPH
1.14.13.2 0.281
-
 at pH 7.5, temperature not specified in the publication Pseudomonas sp. PPD

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + H+ + O2
-
 Alcaligenes sp. PPH protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + H+ + O2
-
 Pseudomonas sp. PPD protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 additional information the enzyme also shows low activities with 2- and 3-hydroxybenzoate as well as D-glucose Alcaligenes sp. PPH ?
-
 ?
1.14.13.2 additional information the enzyme also shows low activities with 2- and 3-hydroxybenzoate as well as D-glucose Pseudomonas sp. PPD ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.2 4-HBA 3-hydroxylase
-
 Alcaligenes sp. PPH
1.14.13.2 4-HBA 3-hydroxylase
-
 Pseudomonas sp. PPD

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD
-
 Alcaligenes sp. PPH
1.14.13.2 FAD
-
 Pseudomonas sp. PPD
1.14.13.2 NADPH
-
 Alcaligenes sp. PPH
1.14.13.2 NADPH
-
 Pseudomonas sp. PPD