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Literature summary extracted from

  • van Beek, H.L.; Wijma, H.J.; Fromont, L.; Janssen, D.B.; Fraaije, M.W.
    Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue (2014), FEBS Open Bio, 4, 168-174 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.22 A255C/A293C mutation results in an apparent Tm increase of 1.5°C Acinetobacter johnsonii
1.14.13.22 A325C/L483C mutation results in an apparent Tm increase of 1.5°C Acinetobacter johnsonii
1.14.13.22 L323C/A325C mutation results in an apparent Tm increase of 6°C and a 12fold increased half-life, the stabilizing disulfide bond, L323C/A325C, spans only one residue Acinetobacter johnsonii
1.14.13.22 R534stop mutation results in an apparent Tm decrease of 1°C Acinetobacter johnsonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.22 0.0007
-
bicyclo[3.2.0]hept-2-en-6-one pH 8.5, 20°C, mutant enzyme L323C/A325C Acinetobacter johnsonii
1.14.13.22 0.0013
-
bicyclo[3.2.0]hept-2-en-6-one pH 8.5, 20°C, wild-type enzyme Acinetobacter johnsonii
1.14.13.22 0.003
-
cyclohexanone pH 8.5, 20°C, mutant enzyme L323C/A325C Acinetobacter johnsonii
1.14.13.22 0.0036
-
cyclohexanone pH 8.5, 20°C, wild-type enzyme Acinetobacter johnsonii
1.14.13.22 0.013
-
NADPH pH 8.5, 20°C, mutant enzyme L323C/A325C Acinetobacter johnsonii
1.14.13.22 0.016
-
NADPH pH 8.5, 20°C, wild-type enzyme Acinetobacter johnsonii

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Acinetobacter johnsonii Q9R2F5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 the enzyme produces all four possible lactones Acinetobacter johnsonii ?
-
?
1.14.13.22 cyclohexanone + NADPH + H+ + O2
-
Acinetobacter johnsonii hexano-6-lactone + NADP+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CHMO
-
Acinetobacter johnsonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.22 37
-
Tm-value of mutant enzyme R534stop Acinetobacter johnsonii
1.14.13.22 38
-
Tm-value of wild-type enzyme Acinetobacter johnsonii
1.14.13.22 40.5
-
Tm-value of mutant enzymes A255C/A293C and A325C/L483C Acinetobacter johnsonii
1.14.13.22 44
-
Tm-value of mutant enzyme L323C/A325C Acinetobacter johnsonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.22 4.9
-
bicyclo[3.2.0]hept-2-en-6-one pH 8.5, 20°C, mutant enzyme L323C/A325C Acinetobacter johnsonii
1.14.13.22 6.1
-
cyclohexanone pH 8.5, 20°C, mutant enzyme L323C/A325C Acinetobacter johnsonii
1.14.13.22 13.4
-
bicyclo[3.2.0]hept-2-en-6-one pH 8.5, 20°C, wild-type enzyme Acinetobacter johnsonii
1.14.13.22 14.2
-
cyclohexanone pH 8.5, 20°C, wild-type enzyme Acinetobacter johnsonii