EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.244 | Act | activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, in a pH-dependent manner. Act increases the Vmax of the enzyme and decreases the Km for methanol, the increase was 23fold at pH 7.4 and 50fold at pH 9.0 | Bacillus methanolicus | |
1.1.1.244 | activator protein ACT | UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, in a pH-dependent manner. Act increases the Vmax of the enzyme and decreases the Km for methanol, the increase is 20fold at pH 7.4 and 9fold at pH 9.0 | Bacillus methanolicus | |
1.1.1.244 | additional information | the enzyme is activated by several Nudix hydrolases, relative activities of Adh enzymes, overview | Bacillus methanolicus | |
1.1.1.244 | NudBC | a Nudix hydrolase from Bacillus coagulans, UniProt ID G2TKZ3 | Bacillus methanolicus | |
1.1.1.244 | NudDK | a Nudix hydrolase from Desulfotomaculum kuznetsovii, UniProt ID F6CJI1 | Bacillus methanolicus | |
1.1.1.244 | NudLF | a Nudix hydrolase from Lysinibacillus fusiformis, UniProt ID D7WXY3 | Bacillus methanolicus | |
1.1.1.244 | NudLS | a Nudix hydrolase from Lysinibacillus sphaericus, UniProt ID B1HRL7 | Bacillus methanolicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.244 | gene mdh, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus methanolicus |
1.1.1.244 | gene mdh2, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus methanolicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.244 | D38G | site-directed mutagenesis, the mutant is active with NADP+ and NAD+ in contrast to the wild-type enzyme | Bacillus methanolicus |
1.1.1.244 | D41G | site-directed mutagenesis, the mutant is active with NADP+ and NAD+ in contrast to the wild-type enzyme, it shows increased activity compared to the wild-type enzyme | Bacillus methanolicus |
1.1.1.244 | S101G | site-directed mutagenesis, the mutant shows altered kinetics, reduced activity, and altered pH-dependency compared to the wild-type enzyme, overview | Bacillus methanolicus |
1.1.1.244 | S98G | site-directed mutagenesis, the mutant shows altered kinetics, reduced activity, and altered pH-dependency compared to the wild-type enzyme, overview | Bacillus methanolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.244 | additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus methanolicus | |
1.1.1.244 | 9 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 25 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 51 | - |
ethanol | recombinant enzyme, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 96 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 137 | - |
methanol | recombinant enzyme mutant D38G, pH 7.4, 30°C, in presence of activator Act, with NADP+ | Bacillus methanolicus | |
1.1.1.244 | 150 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 161 | - |
ethanol | recombinant enzyme, pH 9.0, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 224 | - |
ethanol | recombinant enzyme, pH 9.0, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 225 | - |
ethanol | recombinant enzyme, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 255 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 349 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 379 | - |
methanol | recombinant enzyme mutant D38G, pH 7.4, 30°C, in absence of activator Act, with NADP+ | Bacillus methanolicus | |
1.1.1.244 | 416 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 733 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 847 | - |
methanol | recombinant enzyme mutant S98G, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 1151 | - |
methanol | recombinant enzyme mutant S98G, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.244 | methanol + NAD+ | Bacillus methanolicus | - |
formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | Bacillus methanolicus MGA3 | - |
formaldehyde + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.244 | Bacillus methanolicus | I3DTM5 | - |
- |
1.1.1.244 | Bacillus methanolicus | I3E2P9 | - |
- |
1.1.1.244 | Bacillus methanolicus MGA3 | I3DTM5 | - |
- |
1.1.1.244 | Bacillus methanolicus MGA3 | I3E2P9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.244 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus methanolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.244 | ethanol + NAD+ | - |
Bacillus methanolicus | acetaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | ethanol + NAD+ | - |
Bacillus methanolicus MGA3 | acetaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | - |
Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | - |
Bacillus methanolicus MGA3 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NADP+ | Mdh enzyme mutant D41G, no activity of the wild-type enzyme with NADP(H) | Bacillus methanolicus | formaldehyde + NADPH + H+ | - |
r | |
1.1.1.244 | methanol + NADP+ | Mdh enzyme mutant D98G, no activity of the wild-type enzyme with NADP(H) | Bacillus methanolicus | formaldehyde + NADPH + H+ | - |
r | |
1.1.1.244 | methanol + NADP+ | Mdh enzyme mutant D98G, no activity of the wild-type enzyme with NADP(H) | Bacillus methanolicus MGA3 | formaldehyde + NADPH + H+ | - |
r | |
1.1.1.244 | methanol + NADP+ | Mdh enzyme mutant D41G, no activity of the wild-type enzyme with NADP(H) | Bacillus methanolicus MGA3 | formaldehyde + NADPH + H+ | - |
r | |
1.1.1.244 | additional information | poor activity with 2-propanol and 1,2-popanediol | Bacillus methanolicus | ? | - |
? | |
1.1.1.244 | additional information | poor activity with 2-propanol and 1,2-popanediol | Bacillus methanolicus MGA3 | ? | - |
? | |
1.1.1.244 | n-butanol + NAD+ | - |
Bacillus methanolicus | butyraldehyde + NADH + H+ | - |
r | |
1.1.1.244 | n-butanol + NAD+ | - |
Bacillus methanolicus MGA3 | butyraldehyde + NADH + H+ | - |
r | |
1.1.1.244 | n-propanol + NAD+ | - |
Bacillus methanolicus | propionaldehyde + NADH + H+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.244 | MDH | - |
Bacillus methanolicus |
1.1.1.244 | MDH2 | - |
Bacillus methanolicus |
1.1.1.244 | NAD-dependent methanol dehydrogenase | - |
Bacillus methanolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 30 | - |
assay at | Bacillus methanolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 9 | - |
- |
Bacillus methanolicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.244 | NAD+ | - |
Bacillus methanolicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.244 | evolution | the enzyme belongs to the type III alcohol dehydrogenase (Adh) family | Bacillus methanolicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.244 | 0.00004 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0001 | - |
methanol | recombinant enzyme mutant D38G, pH 7.4, 30°C, in absence of activator Act, with NADP+ | Bacillus methanolicus | |
1.1.1.244 | 0.0003 | - |
methanol | recombinant enzyme mutant S98G, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0003 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0003 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0006 | - |
methanol | recombinant enzyme mutant D38G, pH 7.4, 30°C, in presence of activator Act, with NADP+ | Bacillus methanolicus | |
1.1.1.244 | 0.0006 | - |
ethanol | recombinant enzyme, pH 7.4, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0007 | - |
methanol | recombinant enzyme mutant S98G, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0007 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0008 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0014 | - |
ethanol | recombinant enzyme, pH 9.0, 30°C, in absence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0028 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0068 | - |
methanol | recombinant enzyme, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0074 | - |
ethanol | recombinant enzyme, pH 7.4, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0143 | - |
ethanol | recombinant enzyme, pH 9.0, 30°C, in presence of activator Act | Bacillus methanolicus | |
1.1.1.244 | 0.0353 | - |
methanol | recombinant enzyme, pH 9.0, 30°C, in presence of activator Act | Bacillus methanolicus |