EC Number | General Stability | Organism |
---|---|---|
1.14.13.63 | 3-hydroxyphenylacetate protects against thermal inactivation | Flavobacterium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.63 | 2-Chloromercuri-4-nitrophenol | - |
Flavobacterium sp. | |
1.14.13.63 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Flavobacterium sp. | |
1.14.13.63 | Cl- | competitive to the aromatic substrate | Flavobacterium sp. | |
1.14.13.63 | NEM | - |
Flavobacterium sp. | |
1.14.13.63 | p-hydroxymercuribenzoate | - |
Flavobacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.63 | 0.079 | - |
3-hydroxyphenylacetate | - |
Flavobacterium sp. | |
1.14.13.63 | 0.09 | - |
3,4-Dihydroxyphenylacetate | - |
Flavobacterium sp. | |
1.14.13.63 | 0.14 | - |
NADPH | - |
Flavobacterium sp. | |
1.14.13.63 | 0.15 | - |
NADH | - |
Flavobacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.63 | 63000 | - |
2 * 63000, SDS-PAGE | Flavobacterium sp. |
1.14.13.63 | 150000 | 200000 | gel filtration | Flavobacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.63 | Flavobacterium sp. | - |
- |
- |
1.14.13.63 | Flavobacterium sp. JS-7 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.63 | - |
Flavobacterium sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.63 | culture condition:phenylacetic acid-grown cell | - |
Flavobacterium sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.63 | 20.1 | - |
- |
Flavobacterium sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.63 | -70°C, stable for at least 1 month | Flavobacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.63 | 3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+ | - |
Flavobacterium sp. | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
1.14.13.63 | 3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+ | - |
Flavobacterium sp. JS-7 | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
1.14.13.63 | 3,4-Dihydroxyphenylacetate + NADH + O2 | - |
Flavobacterium sp. | 2,4,5-Trihydroxyphenylacetate + NAD+ | - |
? | |
1.14.13.63 | 3,4-Dihydroxyphenylacetate + NADH + O2 | - |
Flavobacterium sp. JS-7 | 2,4,5-Trihydroxyphenylacetate + NAD+ | - |
? | |
1.14.13.63 | 3-Hydroxyphenylacetate + NADH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
1.14.13.63 | 3-Hydroxyphenylacetate + NADH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. JS-7 | 2,5-Dihydroxyphenylacetate + NAD+ | - |
? | |
1.14.13.63 | 3-Hydroxyphenylacetate + NADPH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. | 2,5-Dihydroxyphenylacetate + NADP+ | - |
? | |
1.14.13.63 | 3-Hydroxyphenylacetate + NADPH + O2 | NADPH and NADH are oxidized at a comparable rate | Flavobacterium sp. JS-7 | 2,5-Dihydroxyphenylacetate + NADP+ | - |
? | |
1.14.13.63 | 4-Hydroxyphenylacetate + NADH + O2 | - |
Flavobacterium sp. | 2,4-Dihydroxyphenylacetate + NAD+ | - |
? | |
1.14.13.63 | additional information | in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH | Flavobacterium sp. | ? | - |
? | |
1.14.13.63 | additional information | in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH | Flavobacterium sp. JS-7 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.63 | dimer | 2 * 63000, SDS-PAGE | Flavobacterium sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.63 | 15.6 | - |
3,4-Dihydroxyphenylacetate | - |
Flavobacterium sp. | |
1.14.13.63 | 22.4 | - |
3-hydroxyphenylacetate | - |
Flavobacterium sp. | |
1.14.13.63 | 35.8 | - |
NADPH | - |
Flavobacterium sp. | |
1.14.13.63 | 36 | - |
NADH | - |
Flavobacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.63 | 8.3 | 8.9 | - |
Flavobacterium sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.63 | 7.3 | 8.9 | 50% of maximal activity at pH 7.3 and 8.9 | Flavobacterium sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.63 | 6.5 | 7 | 37°C, stable | Flavobacterium sp. |
1.14.13.63 | 9 | - |
strong inactivation above | Flavobacterium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.63 | FAD | contains 1 mol non-covalently bound FAD per mol of subunit | Flavobacterium sp. |