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Literature summary extracted from

  • van Berkel, W.J.H.; van den Tweel, W.J.J.
    Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species (1991), Eur. J. Biochem., 201, 585-591.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.63 3-hydroxyphenylacetate protects against thermal inactivation Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.63 2-Chloromercuri-4-nitrophenol
-
Flavobacterium sp.
1.14.13.63 5,5'-dithiobis(2-nitrobenzoate)
-
Flavobacterium sp.
1.14.13.63 Cl- competitive to the aromatic substrate Flavobacterium sp.
1.14.13.63 NEM
-
Flavobacterium sp.
1.14.13.63 p-hydroxymercuribenzoate
-
Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.63 0.079
-
3-hydroxyphenylacetate
-
Flavobacterium sp.
1.14.13.63 0.09
-
3,4-Dihydroxyphenylacetate
-
Flavobacterium sp.
1.14.13.63 0.14
-
NADPH
-
Flavobacterium sp.
1.14.13.63 0.15
-
NADH
-
Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.63 63000
-
2 * 63000, SDS-PAGE Flavobacterium sp.
1.14.13.63 150000 200000 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.63 Flavobacterium sp.
-
-
-
1.14.13.63 Flavobacterium sp. JS-7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.63
-
Flavobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.63 culture condition:phenylacetic acid-grown cell
-
Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.63 20.1
-
-
Flavobacterium sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.63 -70°C, stable for at least 1 month Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+
-
Flavobacterium sp. 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
?
1.14.13.63 3,4-dihydroxyphenylacetate + NAD(P)H + O2 + H+
-
Flavobacterium sp. JS-7 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
?
1.14.13.63 3,4-Dihydroxyphenylacetate + NADH + O2
-
Flavobacterium sp. 2,4,5-Trihydroxyphenylacetate + NAD+
-
?
1.14.13.63 3,4-Dihydroxyphenylacetate + NADH + O2
-
Flavobacterium sp. JS-7 2,4,5-Trihydroxyphenylacetate + NAD+
-
?
1.14.13.63 3-Hydroxyphenylacetate + NADH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. 2,5-Dihydroxyphenylacetate + NAD+
-
?
1.14.13.63 3-Hydroxyphenylacetate + NADH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. JS-7 2,5-Dihydroxyphenylacetate + NAD+
-
?
1.14.13.63 3-Hydroxyphenylacetate + NADPH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. 2,5-Dihydroxyphenylacetate + NADP+
-
?
1.14.13.63 3-Hydroxyphenylacetate + NADPH + O2 NADPH and NADH are oxidized at a comparable rate Flavobacterium sp. JS-7 2,5-Dihydroxyphenylacetate + NADP+
-
?
1.14.13.63 4-Hydroxyphenylacetate + NADH + O2
-
Flavobacterium sp. 2,4-Dihydroxyphenylacetate + NAD+
-
?
1.14.13.63 additional information in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH Flavobacterium sp. ?
-
?
1.14.13.63 additional information in the absence of oxygen the free enzyme is slowly reduced by NADPH or NADH Flavobacterium sp. JS-7 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.63 dimer 2 * 63000, SDS-PAGE Flavobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.63 15.6
-
3,4-Dihydroxyphenylacetate
-
Flavobacterium sp.
1.14.13.63 22.4
-
3-hydroxyphenylacetate
-
Flavobacterium sp.
1.14.13.63 35.8
-
NADPH
-
Flavobacterium sp.
1.14.13.63 36
-
NADH
-
Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.63 8.3 8.9
-
Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.63 7.3 8.9 50% of maximal activity at pH 7.3 and 8.9 Flavobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.63 6.5 7 37°C, stable Flavobacterium sp.
1.14.13.63 9
-
strong inactivation above Flavobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.63 FAD contains 1 mol non-covalently bound FAD per mol of subunit Flavobacterium sp.