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Literature summary extracted from

  • Tongsook, C.; Uhl, M.; Jankowitsch, F.; Mack, M.; Gruber, K.; Macheroux, P.
    Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-D-riboflavin to the antibiotic roseoflavin (2016), FEBS J., 283, 1531-1549 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.343 expressed in Escherichia coli Rosetta 2 (DE3) and BL834 (DE3) cells Streptomyces davaonensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.343 roseoflavin competitive inhibition Streptomyces davaonensis
2.1.1.343 S-adenosyl-L-homocysteine competitive inhibition Streptomyces davaonensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.343 0.004
-
 8-amino-8-demethyl-D-riboflavin at pH 8.0 and 25°C Streptomyces davaonensis
2.1.1.343 0.07
-
 S-adenosyl-L-methionine at pH 8.0 and 25°C Streptomyces davaonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.343 77000
-
 gel filtration Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis
-
 S-adenosyl-L-homocysteine + roseoflavin
-
 ?
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin Streptomyces davaonensis JCM 4913
-
 S-adenosyl-L-homocysteine + roseoflavin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.343 Streptomyces davaonensis K4RFM2
-
-
2.1.1.343 Streptomyces davaonensis JCM 4913 K4RFM2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.343 HisTrap column chromatography and MonoQ column chromatography Streptomyces davaonensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
 Streptomyces davaonensis S-adenosyl-L-homocysteine + roseoflavin
-
 ?
2.1.1.343 S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
-
 Streptomyces davaonensis JCM 4913 S-adenosyl-L-homocysteine + roseoflavin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.343 homodimer 2 * 38000, SDS-PAGE Streptomyces davaonensis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.343 RosA
-
 Streptomyces davaonensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.343 0.001
-
 S-adenosyl-L-methionine at pH 8.0 and 25°C Streptomyces davaonensis
2.1.1.343 0.001
-
 8-amino-8-demethyl-D-riboflavin at pH 8.0 and 25°C Streptomyces davaonensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.343 S-adenosyl-L-methionine
-
 Streptomyces davaonensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.343 0.007
-
 roseoflavin at pH 8.0 and 25°C Streptomyces davaonensis
2.1.1.343 0.027
-
 S-adenosyl-L-homocysteine at pH 8.0 and 25°C Streptomyces davaonensis