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Literature summary extracted from

  • Rosini, E.; Piubelli, L.; Molla, G.; Frattini, L.; Valentino, M.; Varriale, A.; DAuria, S.; Pollegioni, L.
    Novel biosensors based on optimized glycine oxidase (2014), FEBS J., 281, 3460-3472 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli BL21(DE3)pLysS cells Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.19 A54E the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 A54R the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 A54R/H244K the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 A54R/H244K/M261R the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 G51H the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 G51Q the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 H244A the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 H244K the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 H244K/M261R the variant shows a 5.4fold increase in maximal activity on glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 H244N the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 H244Q the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 H244R the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 M261I the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 M261R the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 M49I the mutant shows increased catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 M49L the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 M49L/A54R/H244K the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 M49T the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis
1.4.3.19 Y246W the mutant shows reduced catalytic efficiency with glycine compared to the wild type enzyme Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.14
-
 glycine mutant enzyme H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.24
-
 glycine mutant enzyme H244R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.3
-
 glycine mutant enzyme H244K/M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.31
-
 glycine mutant enzyme M49I, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.7
-
 glycine wild type enzyme, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.7
-
 sarcosine wild type enzyme, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.9
-
 glycine mutant enzyme H244Q, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.35
-
 glycine mutant enzyme M49L, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.47
-
 glycine mutant enzyme M49T, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.5
-
 glycine mutant enzyme H244A, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.5
-
 glycine mutant enzyme M261I, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.9
-
 glycine mutant enzyme H244N, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 3.5
-
 glycine mutant enzyme M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 10
-
 glycine mutant enzyme A54E, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 14.6
-
 glycine mutant enzyme G51Q, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 17.2
-
 glycine mutant enzyme Y246W, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 18.8
-
 glycine mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 28
-
 glycine mutant enzyme A54R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 35.7
-
 glycine mutant enzyme G51H, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 40.1
-
 glycine mutant enzyme A54R/H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 45.9
-
 glycine mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Bacillus subtilis O31616
-
-
1.4.3.19 Bacillus subtilis 168 O31616
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 HiTrap chelating column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 glycine + H2O + O2
-
 Bacillus subtilis glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Bacillus subtilis 168 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Bacillus subtilis glyoxylate + methylamine + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Bacillus subtilis 168 glyoxylate + methylamine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.19 homotetramer 4 * 47000, SDS-PAGE Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.19 57
-
 melting temperature Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.19 0.04
-
 glycine mutant enzyme M49T, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.35
-
 glycine mutant enzyme A54E, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.36
-
 glycine mutant enzyme M49I, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.43
-
 glycine mutant enzyme M49L, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.6
-
 glycine mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.6
-
 glycine wild type enzyme, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.6
-
 sarcosine wild type enzyme, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.63
-
 glycine mutant enzyme H244A, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.64
-
 glycine mutant enzyme M261I, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.74
-
 glycine mutant enzyme G51Q, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.77
-
 glycine mutant enzyme G51H, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1
-
 glycine mutant enzyme M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.2
-
 glycine mutant enzyme A54R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.2
-
 glycine mutant enzyme H244K/M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.21
-
 glycine mutant enzyme H244Q, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.21
-
 glycine mutant enzyme Y246W, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.3
-
 glycine mutant enzyme H244N, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.35
-
 glycine mutant enzyme H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.49
-
 glycine mutant enzyme H244R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.61
-
 glycine mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 3.2
-
 glycine mutant enzyme A54R/H244K, at pH 8.5 and 25°C Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.19 FAD
-
 Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.19 0.02
-
 glycine mutant enzyme G51H, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.03
-
 glycine mutant enzyme M49L/A54R/H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.03
-
 glycine mutant enzyme M49T, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.04
-
 glycine mutant enzyme A54E, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.04
-
 glycine mutant enzyme A54R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.04
-
 glycine mutant enzyme A54R/H244K/M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.05
-
 glycine mutant enzyme G51Q, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.07
-
 glycine mutant enzyme Y246W, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.11
-
 glycine mutant enzyme A54R/H244K, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.3
-
 glycine mutant enzyme M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.32
-
 glycine mutant enzyme M49L, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.42
-
 glycine mutant enzyme H244A, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.43
-
 glycine mutant enzyme M261I, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.68
-
 glycine mutant enzyme H244N, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.86
-
 glycine wild type enzyme, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 0.86
-
 sarcosine wild type enzyme, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.17
-
 glycine mutant enzyme M49I, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 1.34
-
 glycine mutant enzyme H244Q, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 4
-
 glycine mutant enzyme H244K/M261R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 6.22
-
 glycine mutant enzyme H244R, at pH 8.5 and 25°C Bacillus subtilis
1.4.3.19 9.65
-
 glycine mutant enzyme H244K, at pH 8.5 and 25°C Bacillus subtilis