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Literature summary extracted from

  • Simtchouk, S.; Eng, J.L.; Meints, C.E.; Makins, C.; Wolthers, K.R.
    Kinetic analysis of cytochrome P450 reductase from Artemisia annua reveals accelerated rates of NADH-dependent flavin reduction (2013), FEBS J., 280, 6627-6642 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 expressed in Escherichia coli BL21(DE3) cells Artemisia annua

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 2',5'-ADP
-
 Artemisia annua
1.6.2.4 NAD+
-
 Artemisia annua
1.6.2.4 NADP+
-
 Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.00087
-
 ferricytochrome c at pH 7.5 and 25°C Artemisia annua
1.6.2.4 0.00122
-
 NADPH at pH 7.5 and 25°C Artemisia annua
1.6.2.4 26.76
-
 NADH at pH 7.5 and 25°C Artemisia annua

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Artemisia annua
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 NADH + H+ + ferricytochrome c very low activity with NADH Artemisia annua NAD+ + ferricytochrome c
-
 ?
1.6.2.4 NADPH + H+ + ferricytochrome c
-
 Artemisia annua NADP+ + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 75000, SDS-PAGE Artemisia annua
1.6.2.4 ? x * 73275, calculated from amino acid sequence Artemisia annua

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Artemisia annua
1.6.2.4 cytochrome P450 reductase
-
 Artemisia annua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 16.5
-
 NADH at pH 7.5 and 25°C Artemisia annua
1.6.2.4 65.9
-
 NADPH at pH 7.5 and 25°C Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Artemisia annua
1.6.2.4 FMN
-
 Artemisia annua
1.6.2.4 NADPH the enzyme shows a strong preference (85100-fold) for NADPH over NADH Artemisia annua

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.2.4 0.00026
-
 2',5'-ADP at pH 7.5 and 25°C Artemisia annua
1.6.2.4 0.00159
-
 NADP+ at pH 7.5 and 25°C Artemisia annua
1.6.2.4 0.0269
-
 NAD+ at pH 7.5 and 25°C Artemisia annua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6.2.4 0.000615
-
 NADH at pH 7.5 and 25°C Artemisia annua
1.6.2.4 0.0541
-
 NADPH at pH 7.5 and 25°C Artemisia annua