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Literature summary extracted from

  • Lu, J.; Vlamis-Gardikas, A.; Kandasamy, K.; Zhao, R.; Gustafsson, T.; Engstrand, L.; Hoffner, S.; Engman, L.; Holmgren, A.
    Inhibition of bacterial thioredoxin reductase An antibiotic mechanism targeting bacteria lacking glutathione (2013), FASEB J., 27, 1394-1403 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 1-chloro-2,4-dinitrobenzene
-
 Haemonchus contortus
1.8.1.9 2,2'-(ethane-1,2-diyl)di(1,2-benzoselenazol-3(2H)-one) IC50 value for HEK-293T cells 3.4 microg/ml Escherichia coli
1.8.1.9 2,2'-(hexane-1,6-diyl)di(1,2-benzoselenazol-3(2H)-one) IC50 value for HEK-293T cells 2.5 microg/ml Escherichia coli
1.8.1.9 auranofin 15 nM, complete inhibition Haemonchus contortus
1.8.1.9 ebselen competitive, inhibitor reacts with the active site dithiol Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0025
-
 ebsulfur pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 0.006
-
 NADPH pH 7.8, 37°C Haemonchus contortus
1.8.1.9 0.0275
-
 ebsulfur disulfide pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 0.28
-
 5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.8, 37°C Haemonchus contortus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 134000
-
 gel filtration Haemonchus contortus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Escherichia coli
-
-
-
1.8.1.9 Haemonchus contortus
-
 thioredoxin reductase with additional glutathione reductase, EC 1.8.1.7, activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Haemonchus contortus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 0.044
-
 substrate S-nitrosoglutathione, pH 7.8, 37°C Haemonchus contortus
1.8.1.9 0.068
-
 substrate sodium selenite, pH 7.8, 37°C Haemonchus contortus
1.8.1.9 0.495
-
 substrate ebselen, pH 7.8, 37°C Haemonchus contortus
1.8.1.9 6
-
 substrate 5,5'-dithio-bis(2-nitrobenzoic acid), pH 7.8, 37°C Haemonchus contortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Haemonchus contortus 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 ebselen + NADPH + H+
-
 Haemonchus contortus ? + NADP+
-
 ?
1.8.1.9 ebsulfur + NADP+
-
 Escherichia coli ebsulfur disulfide + NADPH + H+
-
 ?
1.8.1.9 ebsulfur disulfide + NADPH + H+
-
 Escherichia coli ebsulfur + NADP+
-
 r
1.8.1.9 S-nitrosoglutathione + NADPH + H+
-
 Haemonchus contortus ? + NADP+
-
 ?
1.8.1.9 sodium selenite + NADPH + H+
-
 Haemonchus contortus ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 67000, SDS-PAGE Haemonchus contortus

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 HCOI_01258400
-
 Haemonchus contortus
1.8.1.9 TrxR3
-
 Haemonchus contortus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.9 40
-
-
 Haemonchus contortus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.9 37
-
 stable for 20 min Haemonchus contortus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 1.7
-
 ebsulfur disulfide pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 7.1
-
 NADPH pH 7.8, 37°C Haemonchus contortus
1.8.1.9 11.7
-
 ebsulfur pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 11.96
-
 5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.8, 37°C Haemonchus contortus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.9 8
-
-
 Haemonchus contortus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADPH
-
 Escherichia coli
1.8.1.9 NADPH
-
 Haemonchus contortus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.9 0.00001
-
 2,2'-(hexane-1,6-diyl)di(1,2-benzoselenazol-3(2H)-one) pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 0.00005
-
 2,2'-(ethane-1,2-diyl)di(1,2-benzoselenazol-3(2H)-one) pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 0.00052
-
 ebselen pH 7.5, temperature not specified in the publication Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.9 0.00225
-
 pH 7.8, 37°C Haemonchus contortus 1-chloro-2,4-dinitrobenzene

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.9 42
-
 5,5'-dithio-bis(2-nitrobenzoic acid) pH 7.8, 37°C Haemonchus contortus
1.8.1.9 60.5
-
 ebsulfur disulfide pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 470
-
 ebsulfur pH 7.5, temperature not specified in the publication Escherichia coli
1.8.1.9 1170
-
 NADPH pH 7.8, 37°C Haemonchus contortus