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Literature summary extracted from

  • Pan, Y.; Carroll, J.; Elbein, A.
    Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme (2002), Eur. J. Biochem., 269, 6091-6100 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.347 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.347 4
-
 D-glucose 6-phosphate with GDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C Mycobacterium tuberculosis
2.4.1.347 7
-
 D-glucose 6-phosphate with UDP-alpha-D-glucose as cosubstrate, at pH 8.0 and 37°C Mycobacterium tuberculosis
2.4.1.347 16
-
 GDP-alpha-D-glucose at pH 8.0 and 37°C Mycobacterium tuberculosis
2.4.1.347 18
-
 UDP-alpha-D-glucose at pH 8.0 and 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.347 Mn2+ enzyme activity is increased about 2fold by 10 mM Mn2+ Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.347 Mycobacterium tuberculosis P9WN11
-
-
2.4.1.347 Mycobacterium tuberculosis H37Rv P9WN11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.347 Ni-NTA His-binding resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.347 ADP-alpha-D-glucose + D-glucose 6-phosphate ADP-alpha-D-glucose is the best substrate Mycobacterium tuberculosis ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 ADP-alpha-D-glucose + D-glucose 6-phosphate ADP-alpha-D-glucose is the best substrate Mycobacterium tuberculosis H37Rv ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 CDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis CDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 CDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv CDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 GDP-alpha-D-glucose + D-glucose 6-phosphate second best substrate Mycobacterium tuberculosis GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 GDP-alpha-D-glucose + D-glucose 6-phosphate second best substrate Mycobacterium tuberculosis H37Rv GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 TDP-alpha-D-glucose + D-glucose 6-phosphate TDP-alpha-D-glucose is the weakest substrate Mycobacterium tuberculosis TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 TDP-alpha-D-glucose + D-glucose 6-phosphate TDP-alpha-D-glucose is the weakest substrate Mycobacterium tuberculosis H37Rv TDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.347 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.347 ? x * 58000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.347 OtsA
-
 Mycobacterium tuberculosis
2.4.1.347 TPS
-
 Mycobacterium tuberculosis