Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yin, Y.; Yang, R.; Wu, J.; Gu, Z.
    Partial purification, characterization and cDNA cloning of aminoaldehyde dehydrogenase in germinated soybean (Glycine max L.) (2013), Eur. Food Res. Technol., 237, 731-738 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.19 DNA and amino acid sequence determination and analysis, sequence comparisons Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.19 2-mercaptoethanol 66% inhibition at 1 mM Glycine max
1.2.1.19 Ba2+ 30% inhibition at 1 mM Glycine max
1.2.1.19 Cu2+ complete inhibition at 1 mM Glycine max
1.2.1.19 EGTA 7.5% inhibition at 1 mM Glycine max
1.2.1.19 Hg2+ 62% inhibition at 1 mM Glycine max
1.2.1.19 L-cysteine 59% inhibition at 1 mM Glycine max
1.2.1.19 Mg2+ 70% inhibition at 1 mM Glycine max
1.2.1.19 additional information EDTA has no significant effect on the enzyme activity Glycine max
1.2.1.19 Ni2+ complete inhibition at 1 mM Glycine max
1.2.1.19 Pb2+ complete inhibition at 1 mM Glycine max
1.2.1.19 Zn2+ 92% inhibition at 1 mM Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.19 Ca2+ no significant effect on the enzyme activity Glycine max
1.2.1.19 Mn2+ no significant effect on the enzyme activity Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Glycine max U5ILS4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 native enzyme 27.22fold from germinated seeds by ammonium sulfate fractionation, desalting gel filtration, and anion exchange chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.19 seed germinated Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.19 13.71
-
 purified native enzyme, substrate 4-aminobutanal, pH 8.0, 37°C Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 3-aminopropionaldehyde + NAD+ + H2O best substrate Glycine max 3-aminopropionate + NADH + 2 H+
-
 ?
1.2.1.19 4-aminobutyraldehyde + NAD+ + H2O
-
 Glycine max 4-aminobutyrate + NADH + 2 H+
-
 ?
1.2.1.19 additional information no activity with betaine aldehyde, propionaldehyde, and acetaldehyde Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.19 ? x * 53720, native enzyme, SDS-PAGE, x * 54790, sequence calculation Glycine max

Synonyms

EC Number Synonyms Comment Organism
1.2.1.19 AMADH
-
 Glycine max
1.2.1.19 aminoaldehyde dehydrogenase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.19 45
-
-
 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.19 20 60 activity range, profile overview Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.19 50 60 purified enzyme, pH 8.0, rapid inactivation above 50°C, loss of almost 50% activity at 60°C Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 8
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.19 3 9 AMADH activity increases with a pH ranging from pH 3.0 to pH 8.0, but it decreases drastically when pH continuously rises from pH 8.0 to pH 10.0. At pH 9.0, the activity decreases by 75% compared to pH 8.0 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+
-
 Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.19 Glycine max sequence calculation
-
 5.16

General Information

EC Number General Information Comment Organism
1.2.1.19 evolution the enzyme is a member of the aldehyde dehydrogenase 10 family Glycine max