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Literature summary extracted from
- Identification of a beta-glucosidase from the Mucor circinelloides genome by peptide pattern recognition (2014), Enzyme Microb. Technol., 67, 47-52 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expression in Pichia pastoris | Mucor circinelloides |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | modeling of structure | Mucor circinelloides |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | additional information | not inhibitory: D-glucose, enzyme retains 84% activity at glucose concentrations up to 140 mM | Mucor circinelloides |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5, 50°C | Mucor circinelloides |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Mucor circinelloides | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-methylumbelliferyl beta-D-glucopyranoside + H2O | - |
Mucor circinelloides | 4-methylumbelliferone + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mucor circinelloides | 4-nitrophenol + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 78240, calculated, x * 75000, SDS-PAGE | Mucor circinelloides |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Mucor circinelloides |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | - |
1 h, 58% residual activity | Mucor circinelloides |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Mucor circinelloides |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Mucor circinelloides | calculated | - |
5.6 |
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Release 2023.1 (January 2023)
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