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Literature summary extracted from

  • Qu, Z.; Bakken, L.R.; Molstad, L.; Frostegard, A.; Bergaust, L.L.
    Transcriptional and metabolic regulation of denitrification in Paracoccus denitrificans allows low but significant activity of nitrous oxide reductase under oxic conditions (2016), Environ. Microbiol., 18, 2951-2963 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.4 gene nosZ, transcription of nosZ takes place concomitantly with that of narG under suboxic conditions. Catalytically functional N2OR is synthesized and active in aerobically raised cells transferred to vials with 7 vol% O2 in headspace, but N2O reduction rates are 10 times higher when anaerobic precultures are subjected to the same conditions. Upon oxygen exposure, there is an incomplete and transient inactivation of N2OR that can be ascribed to its lower ability to compete for electrons compared with terminal oxidases, quantitative real-time PCR enzyme expression analysis Paracoccus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 O2 N2OR being more sensitive to O2 than the other N-oxide reductases. The enzyme's apparent inactivation by O2 may be due to changes in the redox state of the unique Cuz-centre found in N2OR Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu2+ bound in a Cu centre, dependent on. The state of Cuz in extracted N2OR depends on the conditions during purification. Anoxic purification yields the purple, active form of the enzyme, whereas oxic conditions results in a blue form of N2OR with a redox inert Cuz centre, Cuz*. The blue form of N2OR is catalytically inactive, but may be reactivated in vitro by a strong reductant such as reduced methyl viologen Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + 2 reduced cytochrome c Paracoccus denitrificans
-
 nitrogen + H2O + 2 cytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Paracoccus denitrificans
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.7.2.4 N2OR being more sensitive to O2 than the other N-oxide reductases. The enzyme's apparent inactivation by O2 may be due to changes in the redox state of the unique Cuz-centre found in N2OR Paracoccus denitrificans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.2.4 additional information gene nosZ, transcription of nosZ takes place concomitantly with that of narG under suboxic conditions. Catalytically functional N2OR is synthesized and active in aerobically raised cells transferred to vials with 7 vol% O2 in headspace, but N2O reduction rates are 10 times higher when anaerobic precultures are subjected to the same conditions. Upon oxygen exposure, there is an incomplete and transient inactivation of N2OR that can be ascribed to its lower ability to compete for electrons compared with terminal oxidases Paracoccus denitrificans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 additional information determination of detailed gas kinetics and transcription patterns from batch culture experiments with Paracoccus denitrificans, allowing in vivo estimation of electron flow to O2 and N2O under various O2 regimes Paracoccus denitrificans ?
-
 ?
1.7.2.4 nitrous oxide + 2 reduced cytochrome c
-
 Paracoccus denitrificans nitrogen + H2O + 2 cytochrome c
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.4 N2OR
-
 Paracoccus denitrificans
1.7.2.4 nitrous oxide reductase
-
 Paracoccus denitrificans
1.7.2.4 NosZ
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.4 cytochrome c
-
 Paracoccus denitrificans

Expression

EC Number Organism Comment Expression
1.7.2.4 Paracoccus denitrificans aerobically raised cells of Paracoccus denitrificans induce transcription of nosZ immediately after being transferred to intermediate O2 concentration (7 vol% O2 in headspace) in the presence of NO3- resulting in increased catalytically active enzyme levels up

General Information

EC Number General Information Comment Organism
1.7.2.4 physiological function transcriptional and metabolic regulation of denitrification in Paracoccus denitrificans allows low but significant activity of nitrous oxide reductase under oxic conditions Paracoccus denitrificans