BRENDA - Enzyme Database

The FlxABCD-HdrABC proteins correspond to a novel NADH dehydrogenase/heterodisulfide reductase widespread in anaerobic bacteria and involved in ethanol metabolism in Desulfovibrio vulgaris Hildenborough

Ramos, A.R.; Grein, F.; Oliveira, G.P.; Venceslau, S.S.; Keller, K.L.; Wall, J.D.; Pereira, I.A.; Environ. Microbiol. 17, 2288-2305 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.98.1
expressed in Escherichia coli
Desulfovibrio vulgaris
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.7.3
Zn2+
required
Desulfovibrio vulgaris
1.8.98.1
Zn2+
subunit HdrB contains zinc
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Desulfovibrio vulgaris
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Desulfovibrio vulgaris ATCC 29579
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
Desulfovibrio vulgaris
-
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
Desulfovibrio vulgaris ATCC 29579
-
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
Desulfovibrio vulgaris
-
coenzyme B + coenzyme M + methanophenazine
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
Desulfovibrio vulgaris ATCC 29579
-
coenzyme B + coenzyme M + methanophenazine
-
-
?
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
Desulfovibrio vulgaris
-
DsrC protein + methanophenazine
-
-
r
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
Desulfovibrio vulgaris ATCC 29579
-
DsrC protein + methanophenazine
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.6.5.2
Desulfovibrio vulgaris
Q729F2 and Q729F1 and Q729F0
Q729F2 i.e. subunit FlxA, Q729F1 i.e. subunit FlxB, Q729F0 i.e. subunits FlxCD
-
1.6.5.2
Desulfovibrio vulgaris Hildenborough
Q729F2 and Q729F1 and Q729F0
Q729F2 i.e. subunit FlxA, Q729F1 i.e. subunit FlxB, Q729F0 i.e. subunits FlxCD
-
1.8.7.3
Desulfovibrio vulgaris
-
-
-
1.8.7.3
Desulfovibrio vulgaris ATCC 29579
-
-
-
1.8.98.1
Desulfovibrio vulgaris
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.7.3
Q-Sepharose column chromatography and Superdex 200 gel filtration
Desulfovibrio vulgaris
1.8.98.1
Q-Sepharose column chromatography and Superdex 200 gel filtration
Desulfovibrio vulgaris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
742382
Desulfovibrio vulgaris
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
742382
Desulfovibrio vulgaris ATCC 29579
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
-
742382
Desulfovibrio vulgaris
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
-
742382
Desulfovibrio vulgaris ATCC 29579
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris
coenzyme B + coenzyme M + methanophenazine
-
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris ATCC 29579
coenzyme B + coenzyme M + methanophenazine
-
-
-
?
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris
DsrC protein + methanophenazine
-
-
-
r
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris ATCC 29579
DsrC protein + methanophenazine
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.6.5.2
FAD
flavoprotein subunit FlxA is involved in oxidation/reduction of NAD(P)H/NAD(P)+
Desulfovibrio vulgaris
1.8.98.1
FAD
-
Desulfovibrio vulgaris
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.1
expressed in Escherichia coli
Desulfovibrio vulgaris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.6.5.2
FAD
flavoprotein subunit FlxA is involved in oxidation/reduction of NAD(P)H/NAD(P)+
Desulfovibrio vulgaris
1.8.98.1
FAD
-
Desulfovibrio vulgaris
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.7.3
Zn2+
required
Desulfovibrio vulgaris
1.8.98.1
Zn2+
subunit HdrB contains zinc
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Desulfovibrio vulgaris
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
Desulfovibrio vulgaris ATCC 29579
-
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
Desulfovibrio vulgaris
-
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
Desulfovibrio vulgaris ATCC 29579
-
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
Desulfovibrio vulgaris
-
coenzyme B + coenzyme M + methanophenazine
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
Desulfovibrio vulgaris ATCC 29579
-
coenzyme B + coenzyme M + methanophenazine
-
-
?
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
Desulfovibrio vulgaris
-
DsrC protein + methanophenazine
-
-
r
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
Desulfovibrio vulgaris ATCC 29579
-
DsrC protein + methanophenazine
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.7.3
Q-Sepharose column chromatography and Superdex 200 gel filtration
Desulfovibrio vulgaris
1.8.98.1
Q-Sepharose column chromatography and Superdex 200 gel filtration
Desulfovibrio vulgaris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
742382
Desulfovibrio vulgaris
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
742382
Desulfovibrio vulgaris ATCC 29579
oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
-
742382
Desulfovibrio vulgaris
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
-
?
1.8.7.3
reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
-
742382
Desulfovibrio vulgaris ATCC 29579
oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris
coenzyme B + coenzyme M + methanophenazine
-
-
-
?
1.8.98.1
coenzyme B-coenzyme M disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris ATCC 29579
coenzyme B + coenzyme M + methanophenazine
-
-
-
?
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris
DsrC protein + methanophenazine
-
-
-
r
1.8.98.1
DsrC protein disulfide + dihydromethanophenazine
-
742382
Desulfovibrio vulgaris ATCC 29579
DsrC protein + methanophenazine
-
-
-
r
Expression
EC Number
Organism
Commentary
Expression
1.6.5.2
Desulfovibrio vulgaris
expression increases during growth in ethanol-sulfate, and to a less extent during pyruvate fermentation
up
General Information
EC Number
General Information
Commentary
Organism
1.6.5.2
physiological function
a gene disruption strain is unable to grow with ethanol-sulfate. The FlxABCD-HdrABC (heterodisulfide reductase) proteins are essential for NADH oxidation during growth on ethanol, while in fermentation they operate in reverse, reducing NAD+ for ethanol production
Desulfovibrio vulgaris
1.8.98.1
metabolism
the enzyme is involved in ethanol metabolism
Desulfovibrio vulgaris
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.6.5.2
physiological function
a gene disruption strain is unable to grow with ethanol-sulfate. The FlxABCD-HdrABC (heterodisulfide reductase) proteins are essential for NADH oxidation during growth on ethanol, while in fermentation they operate in reverse, reducing NAD+ for ethanol production
Desulfovibrio vulgaris
1.8.98.1
metabolism
the enzyme is involved in ethanol metabolism
Desulfovibrio vulgaris
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.6.5.2
Desulfovibrio vulgaris
expression increases during growth in ethanol-sulfate, and to a less extent during pyruvate fermentation
up