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Literature summary extracted from

  • Ramos, A.R.; Grein, F.; Oliveira, G.P.; Venceslau, S.S.; Keller, K.L.; Wall, J.D.; Pereira, I.A.
    The FlxABCD-HdrABC proteins correspond to a novel NADH dehydrogenase/heterodisulfide reductase widespread in anaerobic bacteria and involved in ethanol metabolism in Desulfovibrio vulgaris Hildenborough (2015), Environ. Microbiol., 17, 2288-2305 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.98.1 expressed in Escherichia coli Nitratidesulfovibrio vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.7.3 Zn2+ required Nitratidesulfovibrio vulgaris
1.8.98.1 Zn2+ subunit HdrB contains zinc Nitratidesulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ Nitratidesulfovibrio vulgaris
-
 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
 ?
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ Nitratidesulfovibrio vulgaris ATCC 29579
-
 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
 ?
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+ Nitratidesulfovibrio vulgaris
-
 oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
 ?
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+ Nitratidesulfovibrio vulgaris ATCC 29579
-
 oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
 ?
1.8.98.1 coenzyme B-coenzyme M disulfide + dihydromethanophenazine Nitratidesulfovibrio vulgaris
-
 coenzyme B + coenzyme M + methanophenazine
-
 ?
1.8.98.1 coenzyme B-coenzyme M disulfide + dihydromethanophenazine Nitratidesulfovibrio vulgaris ATCC 29579
-
 coenzyme B + coenzyme M + methanophenazine
-
 ?
1.8.98.1 DsrC protein disulfide + dihydromethanophenazine Nitratidesulfovibrio vulgaris
-
 DsrC protein + methanophenazine
-
 r
1.8.98.1 DsrC protein disulfide + dihydromethanophenazine Nitratidesulfovibrio vulgaris ATCC 29579
-
 DsrC protein + methanophenazine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Nitratidesulfovibrio vulgaris Q729F2 and Q729F1 and Q729F0 Q729F2 i.e. subunit FlxA, Q729F1 i.e. subunit FlxB, Q729F0 i.e. subunits FlxCD
-
1.6.5.2 Nitratidesulfovibrio vulgaris Hildenborough Q729F2 and Q729F1 and Q729F0 Q729F2 i.e. subunit FlxA, Q729F1 i.e. subunit FlxB, Q729F0 i.e. subunits FlxCD
-
1.8.7.3 Nitratidesulfovibrio vulgaris
-
-
-
1.8.7.3 Nitratidesulfovibrio vulgaris ATCC 29579
-
-
-
1.8.98.1 Nitratidesulfovibrio vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.7.3 Q-Sepharose column chromatography and Superdex 200 gel filtration Nitratidesulfovibrio vulgaris
1.8.98.1 Q-Sepharose column chromatography and Superdex 200 gel filtration Nitratidesulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
 Nitratidesulfovibrio vulgaris oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
 ?
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+
-
 Nitratidesulfovibrio vulgaris ATCC 29579 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM
-
 ?
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
-
 Nitratidesulfovibrio vulgaris oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
 ?
1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+
-
 Nitratidesulfovibrio vulgaris ATCC 29579 oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC
-
 ?
1.8.98.1 coenzyme B-coenzyme M disulfide + dihydromethanophenazine
-
 Nitratidesulfovibrio vulgaris coenzyme B + coenzyme M + methanophenazine
-
 ?
1.8.98.1 coenzyme B-coenzyme M disulfide + dihydromethanophenazine
-
 Nitratidesulfovibrio vulgaris ATCC 29579 coenzyme B + coenzyme M + methanophenazine
-
 ?
1.8.98.1 DsrC protein disulfide + dihydromethanophenazine
-
 Nitratidesulfovibrio vulgaris DsrC protein + methanophenazine
-
 r
1.8.98.1 DsrC protein disulfide + dihydromethanophenazine
-
 Nitratidesulfovibrio vulgaris ATCC 29579 DsrC protein + methanophenazine
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 DVU_2399 locus name, subunit FlxA Nitratidesulfovibrio vulgaris
1.6.5.2 DVU_2400 locus name, subunit FlxB Nitratidesulfovibrio vulgaris
1.6.5.2 DVU_2401 locus name, subunit FlxCD Nitratidesulfovibrio vulgaris
1.6.5.2 FlxABCD
-
 Nitratidesulfovibrio vulgaris
1.8.7.3 HdrABC
-
 Nitratidesulfovibrio vulgaris
1.8.7.3 HdrB subunit Nitratidesulfovibrio vulgaris
1.8.98.1 HdrABC
-
 Nitratidesulfovibrio vulgaris
1.8.98.1 heterodisulfide reductase
-
 Nitratidesulfovibrio vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD flavoprotein subunit FlxA is involved in oxidation/reduction of NAD(P)H/NAD(P)+ Nitratidesulfovibrio vulgaris
1.8.98.1 FAD
-
 Nitratidesulfovibrio vulgaris

Expression

EC Number Organism Comment Expression
1.6.5.2 Nitratidesulfovibrio vulgaris expression increases during growth in ethanol-sulfate, and to a less extent during pyruvate fermentation up

General Information

EC Number General Information Comment Organism
1.6.5.2 physiological function a gene disruption strain is unable to grow with ethanol-sulfate. The FlxABCD-HdrABC (heterodisulfide reductase) proteins are essential for NADH oxidation during growth on ethanol, while in fermentation they operate in reverse, reducing NAD+ for ethanol production Nitratidesulfovibrio vulgaris
1.8.98.1 metabolism the enzyme is involved in ethanol metabolism Nitratidesulfovibrio vulgaris