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Literature summary extracted from

  • Kumar, A.; Tikoo, S.; Maity, S.; Sengupta, S.; Sengupta, S.; Kaur, A.; Bachhawat, A.K.
    Mammalian proapoptotic factor ChaC1 and its homologues function as gamma-glutamyl cyclotransferases acting specifically on glutathione (2012), EMBO Rep., 13, 1095-1101 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.3.2.7 analysis development of a 5-oxoprolinase-coupled, sensitive, 5-oxoproline detection-based gamma-GCT assay. Development of an alternative assay that exploits the Cys-Gly generation from glutathione. Cys-Gly is converted into free cysteine and glycine with the help of Dug1p (a peptidase from Saccharomyces cerevisiae), followed by estimation of cysteine Saccharomyces cerevisiae
4.3.2.7 analysis development of a a 5-oxoprolinase-coupled, sensitive, 5-oxoproline detection-based gamma-GCT assay. Development of an alternative assay that exploits the Cys-Gly generation from glutathione. Cys-Gly is converted into free cysteine and glycine with the help of Dug1p (a peptidase from Saccharomyces cerevisiae), followed by estimation of cysteine Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.7 expression in Escherichia coli Saccharomyces cerevisiae
4.3.2.7 expression in Escherichia coli Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.7 homology modeling of structure Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.7 E115Q mutation of putative catalytic glutamate residue, loss of activity Saccharomyces cerevisiae
4.3.2.7 E116Q mutation of putative catalytic glutamate residue, loss of activity Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.7 1.5 2 glutathione pH 8, 37°C Saccharomyces cerevisiae
4.3.2.7 3.13
-
 glutathione pH 8, 37°C Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.7 glutathione Saccharomyces cerevisiae
-
 L-cysteinylglycine + 5-oxo-L-proline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.7 Mus musculus Q8R3J5
-
-
4.3.2.7 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.7 gamma-glutamyl-L-alanine poor substrate Saccharomyces cerevisiae 5-oxo-L-proline + L-alanine
-
 ?
4.3.2.7 glutathione
-
 Saccharomyces cerevisiae L-cysteinylglycine + 5-oxo-L-proline
-
 ?
4.3.2.7 glutathione
-
 Mus musculus L-cysteinylglycine + 5-oxo-L-proline
-
 ?
4.3.2.7 additional information no substrate: gamma-glutamyl amino acids such as gamma-Glu-Ala, gamma-Glu-Cys, gamma-Glu-Met, gamma-Glu-His and gamma-Glu-Lys Mus musculus ?
-
 ?
4.3.2.7 additional information no substrate: gamma-glutamyl amino acids such as gamma-Glu-Cys, gamma-Glu-Met, gamma-Glu-His and gamma-Glu-Lys Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.2.7 CHAC1
-
 Mus musculus
4.3.2.7 YER63c
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.3.2.7 physiological function gamma-glutamyl cyclotransferase acts specifically to degrade glutathione but not other gamma-glutamyl peptides. The overexpression leads to glutathione depletion and enhanced apoptosis in yeast Saccharomyces cerevisiae
4.3.2.7 physiological function gamma-glutamyl cyclotransferase acts specifically to degrade glutathione but not other gamma-glutamyl peptides. The overexpression leads to glutathione depletion and enhanced apoptosis in yeast Mus musculus