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Literature summary extracted from

  • Li, W.; Gong, M.; Shu, R.; Li, X.; Gao, J.; Meng, Y.
    Molecular and enzymatic characterization of two enzymes BmPCD and BmDHPR involving in the regeneration pathway of tetrahydrobiopterin from the silkworm Bombyx mori (2015), Comp. Biochem. Physiol. B, 186, 20-27 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.34 gene Dhpr, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bombyx mori
4.2.1.96 PCD sequence comparisons and phylogenetic tree, enzyme expression analysis Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.34 additional information generation of lethal mutants of a65 and a60 strains. For a65 (leml/leml) and a60 (al/al), the reaction rate of total proteins is 0.373 and 3.352 microM/min/mg protein, respectively. The expression of BmDhpr is especially activated in the brain and sexual glands of lem mutant and the homozygous lethal mutant of al mutant Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.0314
-
 NADH pH 7.4, 22°C, recombinant enzyme Bombyx mori
1.5.1.34 0.0729
-
 NADPH pH 7.4, 22°C, recombinant enzyme Bombyx mori
1.5.1.34 0.821
-
 6,7-dimethyl-7,8-dihydropteridine pH 7.4, 22°C, recombinant enzyme Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.34 a 6,7-dihydropteridine + NAD(P)H + H+ Bombyx mori
-
 a 5,6,7,8-tetrahydropteridine + NAD(P)+
-
 ?
4.2.1.96 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin Bombyx mori
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Bombyx mori
-
 several strains
-
4.2.1.96 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34 native enzyme from fifth larval instar of p50 and ah09 strains, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 brain
-
 Bombyx mori
-
1.5.1.34 fat body larvaeal Bombyx mori
-
1.5.1.34 larva
-
 Bombyx mori
-
1.5.1.34 additional information the expression of BmDhpr is especially activated in the brain and sexual glands of lem mutant and the homozygous lethal mutant of al mutant Bombyx mori
-
1.5.1.34 sexual gland
-
 Bombyx mori
-
4.2.1.96 brain
-
 Bombyx mori
-
4.2.1.96 imago
-
 Bombyx mori
-
4.2.1.96 larva third, forth, and fifth instar Bombyx mori
-
4.2.1.96 additional information PCD enzyme transcription profiles in different strains, expression analysis, overview. BmPcd is expressed almost throughout the whole life cycle Bombyx mori
-
4.2.1.96 ovariotestis
-
 Bombyx mori
-
4.2.1.96 pupa high expression level Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 6,7-dimethyl-7,8-dihydropteridine + NADH + H+ NADH is preferred in vitro compared to NADPH Bombyx mori 6,7-dimethyl-5,6,7,8-tetrahydropterin + NAD+
-
 ?
1.5.1.34 a 6,7-dihydropteridine + NAD(P)H + H+
-
 Bombyx mori a 5,6,7,8-tetrahydropteridine + NAD(P)+
-
 ?
1.5.1.34 a 6,7-dihydropteridine + NADPH + H+
-
 Bombyx mori a 5,6,7,8-tetrahydropteridine + NADP+
-
 ?
4.2.1.96 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin
-
 Bombyx mori (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.34 ? x * 17900, about, sequence calculation Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
1.5.1.34 BmDhpr
-
 Bombyx mori
1.5.1.34 DHPR
-
 Bombyx mori
1.5.1.34 dihydropteridine reductase
-
 Bombyx mori
4.2.1.96 PCD
-
 Bombyx mori
4.2.1.96 Pterin-4a-carbinolamine dehydratase
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.34 22
-
 assay at room temperature Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.34 7.4
-
 assay at Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 additional information the recombinant BmDHPR exhibits high enzymatic activity and more suitable parameters to the coenzyme of NADH in vitro Bombyx mori
1.5.1.34 NAD(P)H
-
 Bombyx mori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.34 Bombyx mori sequence calculation
-
 9.9

General Information

EC Number General Information Comment Organism
1.5.1.34 malfunction tetrahydrobiopterin deficiency is associated with numerous metabolic syndromes and neuropsychological disorders Bombyx mori
1.5.1.34 metabolism the enzyme is involved in the De novo pathway and regeneration pathway of tetrahydrobiopterin biosynthesis Bombyx mori
1.5.1.34 physiological function the enzyme is important in the regeneration pathway of tetrahydrobiopterin, BH4. Tetrahydrobiopterin (BH4) is an essential cofactor of aromatic amino acid hydroxylases and nitric oxide synthase so that BH4 plays a key role in many biological processes Bombyx mori
4.2.1.96 malfunction in mutant phenotype lemon, the expression of BmDhpr is activated in the brain and sexual glands while BmPcd is expressed in a wider special pattern when the de novo pathway of BH4 is missing Bombyx mori
4.2.1.96 metabolism pterin-4a-carbinolamine dehydratase (PCD, BmPcd) and dihydropteridine reductase (DHPR, BmDhpr) might normally act in the regeneration pathway of Bombyx mori Bombyx mori
4.2.1.96 physiological function the regeneration pathway (RPB) of tetrahydrobiopterin (BH4), an essential cofactor of aromatic amino acid hydroxylases and nitric oxide synthase playing a key role in many biological processes, in Bombyx mori. The enzyme is involved in the insect BH4 biosynthetic networks, overview Bombyx mori