EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.62 | acetone | 15%, 1.2fold activation. Strong decrease in activity above 15% | Pseudomonas putida | |
1.14.13.62 | ethanol | 15%, 1.7fold activation. Strong decrease in activity above 15% | Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.62 | 50% loss of enzyme activity after 96 h without additions | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.62 | 4-chloromercuribenzoate | partial inhibition | Pseudomonas putida | |
1.14.13.62 | Cu2+ | - |
Pseudomonas putida | |
1.14.13.62 | H2O2 | - |
Pseudomonas putida | |
1.14.13.62 | Hg2+ | - |
Pseudomonas putida | |
1.14.13.62 | iodoacetate | partial inhibition | Pseudomonas putida | |
1.14.13.62 | Sodium dithionite | - |
Pseudomonas putida | |
1.14.13.62 | Zn2+ | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.62 | 0.025 | - |
1H-4-Oxoquinoline | - |
Pseudomonas putida | |
1.14.13.62 | 0.087 | - |
NADH | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.62 | 42000 | - |
3 * 42000, SDS-PAGE | Pseudomonas putida |
1.14.13.62 | 126000 | - |
gel filtration | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.62 | additional information | Pseudomonas putida | inducible by 1H-4-oxoquinoline | ? | - |
? | |
1.14.13.62 | additional information | Pseudomonas putida 33/1 | inducible by 1H-4-oxoquinoline | ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.14.13.62 | Acetone | 40% loss of activity after 2 h in 15% acetone | Pseudomonas putida |
1.14.13.62 | Ethanol | stable for 2 h in 15% ethanol, 50% loss of activity after 24 h | Pseudomonas putida |
1.14.13.62 | Ethylene glycol | completely stable after 96 h in 50% ethylene glycol | Pseudomonas putida |
1.14.13.62 | additional information | 50% loss of enzyme activity after 96 h without addition of organic solvents | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.62 | Pseudomonas putida | - |
- |
- |
1.14.13.62 | Pseudomonas putida 33/1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.62 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.62 | 6.8 | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.62 | 1H-4-Oxoquinoline + NADH + O2 | - |
Pseudomonas putida | 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O | - |
? | |
1.14.13.62 | 1H-4-Oxoquinoline + NADH + O2 | - |
Pseudomonas putida 33/1 | 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O | - |
? | |
1.14.13.62 | 1H-4-Oxoquinoline + NADPH + O2 | 50% of the activity with NADH | Pseudomonas putida | 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O | - |
? | |
1.14.13.62 | 1H-4-Oxoquinoline + NADPH + O2 | 50% of the activity with NADH | Pseudomonas putida 33/1 | 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O | - |
? | |
1.14.13.62 | additional information | inducible by 1H-4-oxoquinoline | Pseudomonas putida | ? | - |
? | |
1.14.13.62 | additional information | inducible by 1H-4-oxoquinoline | Pseudomonas putida 33/1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.62 | trimer | 3 * 42000, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.62 | 35 | - |
- |
Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.62 | 7.5 | 8 | - |
Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.62 | 8.5 | - |
strong inactivation above | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.62 | NADH | required as cofactor | Pseudomonas putida | |
1.14.13.62 | NADPH | 50% of the activity compared to NADH | Pseudomonas putida |