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Literature summary extracted from

  • Block, D.W.; Lingens, F.
    XIII. Purification and properties of 1H-4-oxoquinoline monooxygenase from Pseudomonas putida strain 33/1 (1992), Biol. Chem. Hoppe-Seyler, 373, 249-254.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.62 acetone 15%, 1.2fold activation. Strong decrease in activity above 15% Pseudomonas putida
1.14.13.62 ethanol 15%, 1.7fold activation. Strong decrease in activity above 15% Pseudomonas putida

General Stability

EC Number General Stability Organism
1.14.13.62 50% loss of enzyme activity after 96 h without additions Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.62 4-chloromercuribenzoate partial inhibition Pseudomonas putida
1.14.13.62 Cu2+
-
Pseudomonas putida
1.14.13.62 H2O2
-
Pseudomonas putida
1.14.13.62 Hg2+
-
Pseudomonas putida
1.14.13.62 iodoacetate partial inhibition Pseudomonas putida
1.14.13.62 Sodium dithionite
-
Pseudomonas putida
1.14.13.62 Zn2+
-
Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.62 0.025
-
1H-4-Oxoquinoline
-
Pseudomonas putida
1.14.13.62 0.087
-
NADH
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.62 42000
-
3 * 42000, SDS-PAGE Pseudomonas putida
1.14.13.62 126000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.62 additional information Pseudomonas putida inducible by 1H-4-oxoquinoline ?
-
?
1.14.13.62 additional information Pseudomonas putida 33/1 inducible by 1H-4-oxoquinoline ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.13.62 Acetone 40% loss of activity after 2 h in 15% acetone Pseudomonas putida
1.14.13.62 Ethanol stable for 2 h in 15% ethanol, 50% loss of activity after 24 h Pseudomonas putida
1.14.13.62 Ethylene glycol completely stable after 96 h in 50% ethylene glycol Pseudomonas putida
1.14.13.62 additional information 50% loss of enzyme activity after 96 h without addition of organic solvents Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.62 Pseudomonas putida
-
-
-
1.14.13.62 Pseudomonas putida 33/1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.62
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.62 6.8
-
-
Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.62 1H-4-Oxoquinoline + NADH + O2
-
Pseudomonas putida 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O
-
?
1.14.13.62 1H-4-Oxoquinoline + NADH + O2
-
Pseudomonas putida 33/1 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O
-
?
1.14.13.62 1H-4-Oxoquinoline + NADPH + O2 50% of the activity with NADH Pseudomonas putida 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O
-
?
1.14.13.62 1H-4-Oxoquinoline + NADPH + O2 50% of the activity with NADH Pseudomonas putida 33/1 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O
-
?
1.14.13.62 additional information inducible by 1H-4-oxoquinoline Pseudomonas putida ?
-
?
1.14.13.62 additional information inducible by 1H-4-oxoquinoline Pseudomonas putida 33/1 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.62 trimer 3 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.62 35
-
-
Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.62 7.5 8
-
Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.62 8.5
-
strong inactivation above Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.62 NADH required as cofactor Pseudomonas putida
1.14.13.62 NADPH 50% of the activity compared to NADH Pseudomonas putida