EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.3.7 | C187A | the mutation leads to an increase of enzyme activity | Thermomonospora curvata |
1.8.3.7 | C187A/C231A/C284S/C298A | the mutations lead to an increase of enzyme activity | Thermomonospora curvata |
1.8.3.7 | C187A/C231A/Y273F/C284S/C298A | the mutations lead to an increase of enzyme activity | Thermomonospora curvata |
1.8.3.7 | C187A/C284S/C298A | the mutations lead to an increase of enzyme activity | Thermomonospora curvata |
1.8.3.7 | C298A | the in vitro activity is not affected by the mutation | Thermomonospora curvata |
1.8.3.7 | Y273F | the mutation leads to an increase of enzyme activity | Thermomonospora curvata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.7 | 0.1 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/Y273F/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.21 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.28 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.5 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant Y273F, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.55 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0 and 25°C | Thermomonospora curvata |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.3.7 | Cu2+ | 0.002 mM used in assay conditions | Thermomonospora curvata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.7 | a [sulfatase]-L-cysteine + O2 + a thiol | Thermomonospora curvata | - |
a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.3.7 | Thermomonospora curvata | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.7 | a [sulfatase]-L-cysteine + O2 + a thiol | - |
Thermomonospora curvata | a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O | - |
? | |
1.8.3.7 | Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + O2 + hydrogen sulfide + oxidized dithiothreitol | - |
Thermomonospora curvata | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.3.7 | FGE | - |
Thermomonospora curvata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.7 | 0.027 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.065 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.07 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.17 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant Y273F, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.18 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/Y273F/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.7 | 0.0483 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.233 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.316 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant Y273F, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 0.333 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata | |
1.8.3.7 | 1.833 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/Y273F/C284S/C298A, at pH 8.0 and 25°C | Thermomonospora curvata |