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Literature summary extracted from

  • Knop, M.; Dang, T.Q.; Jeschke, G.; Seebeck, F.P.
    Copper is a cofactor of the formylglycine-generating enzyme (2017), ChemBioChem, 18, 161-165 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.7 C187A/C231A/C284S/C298A the mutant shows increased activity compared to the wild type enzyme Thermomonospora curvata
1.8.3.7 C274S inactive Thermomonospora curvata
1.8.3.7 C296S inactive Thermomonospora curvata
1.8.3.7 S266A the mutant shows reduced activity compared to the wild type enzyme Thermomonospora curvata
1.8.3.7 S290K the mutant shows very low activity compared to the wild type enzyme Thermomonospora curvata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.7 0.23
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.52
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant S266A, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.58
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 wild type enzyme, at pH 8.0, temperature not specified in the publication Thermomonospora curvata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.7 Cu+ required Thermomonospora curvata

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.7 Thermomonospora curvata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.7 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + O2 + hydrogen sulfide + oxidized dithiothreitol
-
 Thermomonospora curvata ?
-
 ?
1.8.3.7 additional information low activity with Abz-SALSSPTRA-NH2 Thermomonospora curvata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.3.7 FGE
-
 Thermomonospora curvata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.7 0.0001
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant S266A, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.027
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 wild type enzyme, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.07
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication Thermomonospora curvata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.3.7 0.00011
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant S290K, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.00082
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant S266A, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.048
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 wild type enzyme, at pH 8.0, temperature not specified in the publication Thermomonospora curvata
1.8.3.7 0.333
-
 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication Thermomonospora curvata