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Literature summary extracted from

  • Liu, W.; Li, Z.; Huang, C.H.; Guo, R.T.; Zhao, L.; Zhang, D.; Chen, X.; Wu, Q.; Zhu, D.
    Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum (2014), ChemBioChem, 15, 217-222 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.16 expressed in Escherichia coli BL21(DE3) cells Symbiobacterium thermophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.16 apo form and in complex with NADP+ and both NADPH and meso-2,6-diaminoheptanedioate, sitting drop vapor diffusion method, using 0.1m HEPES buffer (pH 7.5) with 30% 2-methyl-2,4-pentanediol and 5% (v/v) glycerol Symbiobacterium thermophilum

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.16 M152A the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 M152D inactive Symbiobacterium thermophilum
1.4.1.16 M152E inactive Symbiobacterium thermophilum
1.4.1.16 M152H the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and no activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 M152K the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 M152L the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 M152N the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 M152S the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme Symbiobacterium thermophilum
1.4.1.16 M152W inactive Symbiobacterium thermophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ Symbiobacterium thermophilum
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Symbiobacterium thermophilum Q67PI3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.16 Ni-NTA column chromatography and Superdex 200 gel filtration Symbiobacterium thermophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Symbiobacterium thermophilum L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
1.4.1.16 pyruvate + NADPH
-
 Symbiobacterium thermophilum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.16 DAPDH
-
 Symbiobacterium thermophilum
1.4.1.16 meso-diaminopimelate dehydrogenase
-
 Symbiobacterium thermophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NADP+
-
 Symbiobacterium thermophilum