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Literature summary extracted from

  • Jia, H.; Gao, Z.; Ma, Y.; Zhong, C.; Wang, C.; Zhou, H.; Wei, P.
    Preparation and characterization of a highly stable phenoxazinone synthase nanogel (2016), Chem. Cent. J., 10, 34 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.4 DMSO the native enzyme shows less than 5% residual activity in the presence of DMSO Streptomyces antibioticus
1.10.3.4 ethanol the native enzyme shows less than 5% residual activity in the presence of ethanol Streptomyces antibioticus
1.10.3.4 ethyl acetate the native enzyme shows less than 5% residual activity in the presence of ethyl acetate Streptomyces antibioticus
1.10.3.4 methanol the native enzyme shows less than 5% residual activity in the presence of methanol Streptomyces antibioticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.4 0.052
-
 2-Aminophenol enzyme on nanogel, at pH 4.5 and 25°C Streptomyces antibioticus
1.10.3.4 0.077
-
 2-Aminophenol free enzyme, at pH 4.5 and 25°C Streptomyces antibioticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.4 Co2+ the enzyme is a multi-copper oxidase Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.4 2-aminophenol + O2 Streptomyces antibioticus
-
 2-aminophenoxazin-3-one + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.4 Streptomyces antibioticus Q53692
-
-

Storage Stability

EC Number Storage Stability Organism
1.10.3.4 4°C, enzyme on nanogel, 5 weeks, no loss of activity Streptomyces antibioticus
1.10.3.4 4°C, lyophilized enzyme, 5 weeks, 65% loss of activity Streptomyces antibioticus
1.10.3.4 4°C, native enzyme at pH 4.5, 5 weeks, 98% loss of activity Streptomyces antibioticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.4 2,2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) + O2
-
 Streptomyces antibioticus ?
-
 ?
1.10.3.4 2-aminophenol + O2
-
 Streptomyces antibioticus 2-aminophenoxazin-3-one + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.4 phenoxazinone synthase
-
 Streptomyces antibioticus
1.10.3.4 PHS
-
 Streptomyces antibioticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.4 40
-
 free enzyme Streptomyces antibioticus
1.10.3.4 50
-
 enzyme on nanogel Streptomyces antibioticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.4 60
-
 the native enzyme loses about 90% of its activity whereas the enzyme on nanogel loses about 50% of its activity after 2 h preincubation at 60°C Streptomyces antibioticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.4 4 4.5 enzyme on nanogel Streptomyces antibioticus
1.10.3.4 4.5
-
 free enzyme Streptomyces antibioticus