Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.; Kitaoka, M.; Hayashi, K.; Kim, C.; Cote, G.
    Purification and characterization of an intracellular cycloalternan-degrading enzyme from Bacillus sp. NRRL B-21195 (2004), Carbohydr. Res., 339, 1179-1184 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.204 1.68
-
 cyclo[(->6)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->6)-alpha-D-Glc-(->3)-alpha-D-Glc-(1->)] pH 7.0, 37°C Bacillus sp. (in: Bacteria)
3.2.1.204 8.23
-
 panose pH 7.0, 37°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.204 120000
-
 gel filtration Bacillus sp. (in: Bacteria)
3.2.1.204 125000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.204 alpha-isomaltosyl-(1->3)-isomaltose + H2O Bacillus sp. (in: Bacteria)
-
 2 isomaltose
-
 ?
3.2.1.204 cyclobis-(1->6)-alpha-nigerosyl + H2O Bacillus sp. (in: Bacteria)
-
 alpha-isomaltosyl-(1->3)-isomaltose
-
 ?
3.2.1.204 cyclo[(->6)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->6)-alpha-D-Glc-(->3)-alpha-D-Glc-(1->)] + 2 H2O Bacillus sp. (in: Bacteria) i.e. cyclobis-(->6)-alpha-nigerosyl 2 isomaltose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.204 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.204
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.204 alpha-isomaltosyl-(1->3)-alpha-D-glucose + H2O
-
 Bacillus sp. (in: Bacteria) isomaltose + alpha-D-glucopyranose
-
 ?
3.2.1.204 alpha-isomaltosyl-(1->3)-isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) 2 isomaltose
-
 ?
3.2.1.204 cyclobis-(1->6)-alpha-nigerosyl + H2O
-
 Bacillus sp. (in: Bacteria) alpha-isomaltosyl-(1->3)-isomaltose
-
 ?
3.2.1.204 cyclo[(->6)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->6)-alpha-D-Glc-(->3)-alpha-D-Glc-(1->)] + 2 H2O i.e. cyclobis-(->6)-alpha-nigerosyl Bacillus sp. (in: Bacteria) 2 isomaltose
-
 ?
3.2.1.204 cyclo[(->6)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->6)-alpha-D-Glc-(->3)-alpha-D-Glc-(1->)] + 2 H2O i.e. cyclobis-(->6)-alpha-nigerosyl, best substrate Bacillus sp. (in: Bacteria) 2 isomaltose
-
 ?
3.2.1.204 additional information no substrates: maltooligosaccharides or isomaltooligosaccharides. Enzyme requires alpha-isomaltosyl residues connected with (1->4)- or (1->3)-linkages, and prefers (1->4)- over (1->3)-linkages Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.204 panose + H2O
-
 Bacillus sp. (in: Bacteria) isomaltose + alpha-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.204 CADE
-
 Bacillus sp. (in: Bacteria)
3.2.1.204 cycloalternan isomaltosylhydrolase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.204 35
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.204 50
-
 stable up to Bacillus sp. (in: Bacteria)
3.2.1.204 55
-
 30 min, 20% residual activity Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.204 10.8
-
 cyclo[(->6)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->6)-alpha-D-Glc-(->3)-alpha-D-Glc-(1->)] pH 7.0, 37°C Bacillus sp. (in: Bacteria)
3.2.1.204 14.4
-
 panose pH 7.0, 37°C Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.204 7
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.204 5
-
 1 h, 4°C, 70% loss of activity Bacillus sp. (in: Bacteria)
3.2.1.204 6 9.2
-
 Bacillus sp. (in: Bacteria)
3.2.1.204 9.7
-
 1 h, 4°C, 37% loss of activity Bacillus sp. (in: Bacteria)

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.204 1.76
-
 panose pH 7.0, 37°C Bacillus sp. (in: Bacteria)
3.2.1.204 6.44
-
 cyclo[(->6)-alpha-D-Glc-(1->3)-alpha-D-Glc-(1->6)-alpha-D-Glc-(->3)-alpha-D-Glc-(1->)] pH 7.0, 37°C Bacillus sp. (in: Bacteria)