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Literature summary extracted from

  • Bennett, N.A.; Ryan, J.; Biely, P.; Vrsanska, M.; Kremnicky, L.; Macris, B.J.; Kekos, D.; Christakopoulos, P.; Katapodis, P.; Claeyssens, M.; Nerinckx, W.; Ntauma, P.; Bhat, M.K.
    Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882 (1998), Carbohydr. Res., 306, 445-455 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Thermomyces lanuginosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 26300
-
 PAGE Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermomyces lanuginosus
-
-
-
3.2.1.8 Thermomyces lanuginosus ATCC 46882
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Thermomyces lanuginosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.8 culture filtrate
-
 Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 889
-
 pH 6.0, 50°C Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood 4-O-methyl-D-glucuronoxylan + H2O
-
 Thermomyces lanuginosus xylose + xylobiose + ?
-
 ?
3.2.1.8 beechwood 4-O-methyl-D-glucuronoxylan + H2O
-
 Thermomyces lanuginosus ATCC 46882 xylose + xylobiose + ?
-
 ?
3.2.1.8 additional information no substrates: 4-nitrophenyl beta-D--xyloside, 4-nitrophenyl beta-cellobioside Thermomyces lanuginosus ?
-
 ?
3.2.1.8 additional information no substrates: 4-nitrophenyl beta-D--xyloside, 4-nitrophenyl beta-cellobioside Thermomyces lanuginosus ATCC 46882 ?
-
 ?
3.2.1.8 O-acetyl-4-O-methyl-D-glucuronoxylan + H2O
-
 Thermomyces lanuginosus xylose + xylobiose + ?
-
 ?
3.2.1.8 O-acetyl-4-O-methyl-D-glucuronoxylan + H2O
-
 Thermomyces lanuginosus ATCC 46882 xylose + xylobiose + ?
-
 ?
3.2.1.8 rhodymenan + H2O
-
 Thermomyces lanuginosus xylose + xylobiose + ?
-
 ?
3.2.1.8 rhodymenan + H2O
-
 Thermomyces lanuginosus ATCC 46882 xylose + xylobiose + ?
-
 ?
3.2.1.8 xylotriose + H2O
-
 Thermomyces lanuginosus xylobiose + ?
-
 ?
3.2.1.8 xylotriose + H2O
-
 Thermomyces lanuginosus ATCC 46882 xylobiose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 27500, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
-
 Thermomyces lanuginosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 85
-
 more than 50% of maximum activity Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 55
-
 pH 10, stable for 5 h Thermomyces lanuginosus
3.2.1.8 60
-
 pH 5-9, stable for 5 h Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6 6.5
-
 Thermomyces lanuginosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5.5 9 more than 50% of maximum activity Thermomyces lanuginosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 9 stable Thermomyces lanuginosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Thermomyces lanuginosus isoelectric focusing
-
 3.7