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Literature summary extracted from

  • Triches Damaso, M.; Carvalho Andrade, C.; Pereira Jr., N.
    Production and properties of the cellulase-free xylanase from Thermomyces lanuginosus IOC-4145 (2002), Braz. J. Microbiol., 33, 333-338 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol 5 mM, 140% of initial activity Thermomyces lanuginosus
3.2.1.8 dithiothreitol 5 mM, 113% of initial activity Thermomyces lanuginosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ba2+ 5 mM, 88% residual activity Thermomyces lanuginosus
3.2.1.8 Fe3+ 5 mM, 78% residual activity Thermomyces lanuginosus
3.2.1.8 Mg2+ 5 mM, 74% residual activity Thermomyces lanuginosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Thermomyces lanuginosus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 K+ 5 mM, 113% of initial activity Thermomyces lanuginosus
3.2.1.8 Na+ 5 mM, 110% of initial activity Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermomyces lanuginosus
-
-
-
3.2.1.8 Thermomyces lanuginosus IOC-4145
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.8 -20°C, crude culture, 30 days, 15-20% loss of activity, maintaining stable thereafter for 5 months Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O 77% of the activtiy with oat spelt xylan Thermomyces lanuginosus ?
-
 ?
3.2.1.8 beechwood xylan + H2O 77% of the activtiy with oat spelt xylan Thermomyces lanuginosus IOC-4145 ?
-
 ?
3.2.1.8 birchwood xylan + H2O 84% of the activtiy with oat spelt xylan Thermomyces lanuginosus ?
-
 ?
3.2.1.8 birchwood xylan + H2O 84% of the activtiy with oat spelt xylan Thermomyces lanuginosus IOC-4145 ?
-
 ?
3.2.1.8 carboxymethyl cellulose + H2O
-
 Thermomyces lanuginosus ?
-
 ?
3.2.1.8 carboxymethyl cellulose + H2O
-
 Thermomyces lanuginosus IOC-4145 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Thermomyces lanuginosus ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Thermomyces lanuginosus IOC-4145 ?
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 75
-
 assay at Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 24 h, 50% residual activity Thermomyces lanuginosus
3.2.1.8 60
-
 4 h, 50% residual activity Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
 assay at Thermomyces lanuginosus

General Information

EC Number General Information Comment Organism
3.2.1.8 physiological function maximum peaks of xylanase production and specific activity occurr after 96 hours of cultivation in liquid medium using 3% (w/v) corncob as substrate Thermomyces lanuginosus